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OPENSEQ.org
GSIA - Glutathione import ATP-binding protein GsiA
UniProt: P75796 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13472
Length: 623 (551)
Sequences: 7335
Seq/Len: 13.31
GSIA
Paralog alert: 0.77 [within 20: 0.09] - ratio of genomes with paralogs
Cluster includes: ALSA ARAG GSIA LSRA MGLA MODF RBSA UUP XYLG YBHF YBIT YEJF YHES YJJK YPHE YTFR
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
43_E 238_R 5.746 1.00
318_N 342_K 5.108 1.00
33_R 246_E 4.921 1.00
315_R 346_D 4.857 1.00
351_E 533_R 4.797 1.00
17_N 34_N 4.564 1.00
317_R 380_E 4.379 1.00
16_E 77_K 4.344 1.00
14_A 38_S 4.306 1.00
77_K 89_E 4.007 1.00
367_G 520_I 3.789 1.00
341_E 541_Q 3.617 1.00
316_V 319_L 3.609 1.00
45_L 240_L 3.539 1.00
380_E 387_R 3.491 1.00
315_R 382_I 3.372 1.00
402_D 483_K 3.328 1.00
15_V 18_L 3.248 1.00
30_A 33_R 3.183 1.00
19_N 34_N 3.147 1.00
145_E 148_R 3.102 1.00
353_L 535_A 3.076 1.00
14_A 79_L 3.029 1.00
46_A 236_A 3.001 1.00
354_S 531_S 2.995 1.00
252_T 255_Q 2.946 1.00
316_V 345_F 2.914 1.00
349_P 483_K 2.835 1.00
83_R 188_A 2.783 1.00
105_D 188_A 2.734 1.00
447_L 457_A 2.732 1.00
17_N 36_S 2.7 1.00
143_M 163_S 2.689 1.00
318_N 344_S 2.642 1.00
384_N 483_K 2.625 1.00
368_R 374_V 2.598 1.00
320_V 342_K 2.582 1.00
547_P 550_A 2.53 1.00
89_E 92_E 2.522 1.00
15_V 37_F 2.48 1.00
40_Q 43_E 2.471 1.00
94_N 97_Q 2.422 1.00
138_S 141_E 2.385 1.00
508_D 512_D 2.37 1.00
212_K 234_E 2.354 1.00
464_F 472_I 2.337 1.00
446_L 479_A 2.245 1.00
152_Q 218_M 2.221 1.00
39_L 189_V 2.211 1.00
317_R 382_I 2.198 1.00
321_T 369_A 2.187 1.00
59_A 225_I 2.173 1.00
521_S 527_V 2.16 1.00
21_A 30_A 2.157 1.00
211_I 222_V 2.145 1.00
367_G 486_I 2.135 1.00
535_A 545_I 2.131 1.00
251_G 256_I 2.113 1.00
19_N 30_A 2.11 1.00
141_E 145_E 2.093 1.00
16_E 79_L 2.058 1.00
240_L 247_A 2.056 1.00
366_T 537_M 2.055 1.00
348_W 351_E 2.033 1.00
41_R 188_A 1.997 1.00
519_F 530_I 1.995 1.00
435_P 438_D 1.951 1.00
535_A 542_I 1.93 1.00
445_W 449_R 1.921 1.00
338_H 341_E 1.913 1.00
238_R 250_T 1.882 1.00
22_F 66_L 1.871 1.00
369_A 376_S 1.86 1.00
347_L 353_L 1.85 1.00
39_L 45_L 1.832 1.00
347_L 484_V 1.826 1.00
345_F 535_A 1.816 1.00
145_E 186_R 1.807 1.00
320_V 378_G 1.774 1.00
149_M 184_S 1.754 1.00
449_R 508_D 1.749 1.00
317_R 346_D 1.747 1.00
147_K 151_D 1.745 1.00
151_D 159_Q 1.737 1.00
312_P 315_R 1.723 1.00
144_V 148_R 1.719 1.00
16_E 38_S 1.712 1.00
152_Q 213_V 1.706 1.00
236_A 253_V 1.705 1.00
213_V 217_E 1.702 1.00
37_F 240_L 1.691 1.00
425_I 439_A 1.681 1.00
88_I 97_Q 1.678 1.00
533_R 545_I 1.676 1.00
17_N 75_C 1.676 1.00
16_E 36_S 1.668 1.00
383_F 484_V 1.66 1.00
127_I 184_S 1.656 1.00
161_I 164_R 1.645 1.00
12_V 221_G 1.637 1.00
311_E 349_P 1.636 1.00
406_I 487_A 1.625 1.00
442_R 445_W 1.604 1.00
141_E 144_V 1.603 1.00
15_V 62_L 1.598 1.00
92_E 104_A 1.591 1.00
414_L 461_P 1.59 1.00
312_P 348_W 1.588 1.00
546_G 551_V 1.584 1.00
316_V 370_L 1.557 1.00
164_R 168_Q 1.545 1.00
318_N 378_G 1.53 1.00
483_K 515_I 1.523 1.00
446_L 475_A 1.521 1.00
372_R 396_L 1.512 1.00
80_L 189_V 1.5 1.00
147_K 162_L 1.497 1.00
212_K 215_Q 1.496 1.00
152_Q 217_E 1.491 1.00
128_A 142_A 1.491 1.00
349_P 516_A 1.478 1.00
421_G 458_W 1.477 1.00
494_L 502_I 1.468 1.00
441_A 445_W 1.457 1.00
449_R 513_F 1.454 1.00
22_F 57_V 1.447 1.00
143_M 147_K 1.446 1.00
419_T 422_D 1.436 1.00
314_L 484_V 1.411 1.00
417_R 462_H 1.397 0.99
353_L 518_L 1.395 0.99
35_L 247_A 1.393 0.99
188_A 220_M 1.387 0.99
558_P 561_R 1.381 0.99
322_R 338_H 1.381 0.99
186_R 218_M 1.373 0.99
444_A 457_A 1.372 0.99
93_Q 97_Q 1.372 0.99
109_I 192_A 1.367 0.99
240_L 250_T 1.365 0.99
440_A 444_A 1.364 0.99
355_L 518_L 1.355 0.99
34_N 246_E 1.349 0.99
415_D 426_E 1.344 0.99
347_L 516_A 1.342 0.99
122_T 125_E 1.341 0.99
438_D 441_A 1.339 0.99
546_G 550_A 1.33 0.99
13_L 62_L 1.329 0.99
13_L 80_L 1.323 0.99
249_E 271_A 1.321 0.99
317_R 344_S 1.316 0.99
345_F 366_T 1.315 0.99
80_L 83_R 1.311 0.99
255_Q 259_A 1.307 0.99
80_L 106_M 1.302 0.99
79_L 89_E 1.297 0.99
170_S 173_M 1.286 0.99
312_P 346_D 1.279 0.99
352_T 506_L 1.278 0.99
418_Q 422_D 1.276 0.99
447_L 454_P 1.276 0.99
420_I 472_I 1.275 0.99
165_Y 168_Q 1.273 0.99
354_S 527_V 1.272 0.99
148_R 152_Q 1.264 0.99
537_M 542_I 1.264 0.99
312_P 349_P 1.257 0.99
128_A 146_A 1.256 0.99
11_N 40_Q 1.254 0.99
41_R 221_G 1.248 0.99
157_E 160_T 1.246 0.98
421_G 457_A 1.24 0.98
63_M 108_M 1.239 0.98
444_A 448_E 1.236 0.98
372_R 375_E 1.235 0.98
321_T 377_Q 1.235 0.98
347_L 518_L 1.234 0.98
352_T 510_Q 1.233 0.98
403_I 484_V 1.232 0.98
44_T 211_I 1.23 0.98
340_V 369_A 1.228 0.98
226_T 232_V 1.224 0.98
251_G 255_Q 1.224 0.98
20_I 61_A 1.216 0.98
18_L 61_A 1.208 0.98
382_I 387_R 1.195 0.98
352_T 531_S 1.192 0.98
40_Q 221_G 1.192 0.98
19_N 75_C 1.191 0.98
445_W 448_E 1.189 0.98
355_L 535_A 1.187 0.98
340_V 343_V 1.181 0.98
22_F 31_A 1.177 0.98
442_R 481_N 1.176 0.98
342_K 541_Q 1.169 0.97
45_L 250_T 1.168 0.97
506_L 517_Y 1.166 0.97
527_V 531_S 1.159 0.97
215_Q 237_D 1.157 0.97
425_I 443_V 1.152 0.97
40_Q 238_R 1.148 0.97
21_A 74_Q 1.145 0.97
127_I 162_L 1.142 0.97
19_N 33_R 1.141 0.97
383_F 388_I 1.14 0.97
320_V 338_H 1.136 0.97
45_L 238_R 1.135 0.97
247_A 250_T 1.13 0.97
392_S 395_K 1.129 0.97
205_A 234_E 1.128 0.97
174_R 409_D 1.126 0.97
107_A 182_A 1.122 0.97
155_I 161_I 1.121 0.97
421_G 440_A 1.119 0.96
79_L 83_R 1.117 0.96
209_Q 234_E 1.115 0.96
209_Q 213_V 1.107 0.96
65_L 110_F 1.1 0.96
192_A 211_I 1.099 0.96
550_A 554_N 1.099 0.96
21_A 75_C 1.098 0.96
461_P 472_I 1.098 0.96
12_V 189_V 1.097 0.96
127_I 146_A 1.091 0.96
122_T 165_Y 1.088 0.96
208_L 231_V 1.086 0.96
424_I 452_L 1.085 0.96
343_V 366_T 1.081 0.95
486_I 520_I 1.081 0.95
353_L 545_I 1.075 0.95
313_V 384_N 1.075 0.95
45_L 223_I 1.073 0.95
120_V 167_H 1.071 0.95
319_L 369_A 1.069 0.95
320_V 341_E 1.068 0.95
190_L 220_M 1.068 0.95
37_F 58_T 1.065 0.95
64_R 98_M 1.054 0.95
316_V 381_I 1.054 0.95
148_R 151_D 1.05 0.94
321_T 374_V 1.049 0.94
549_R 553_E 1.045 0.94
154_R 213_V 1.039 0.94
371_L 486_I 1.03 0.94
440_A 458_W 1.021 0.93
61_A 78_M 1.02 0.93
449_R 512_D 1.019 0.93
481_N 513_F 1.013 0.93
356_V 527_V 1.012 0.93
322_R 377_Q 1.01 0.93
150_L 162_L 1.01 0.93
124_G 143_M 1.009 0.93
154_R 206_Q 1.003 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3g5uA20.84911000.375Contact Map0.661
4f4cA10.84111000.38Contact Map0.722
4finA20.79941000.395Contact Map0.719
2iw3A20.75761000.442Contact Map0.709
1yqtA10.82991000.448Contact Map0.613
3ux8A10.81381000.45Contact Map0.616
3ozxA20.83311000.452Contact Map0.627
3bk7A10.82341000.455Contact Map0.601
3j16B10.83471000.462Contact Map0.405
2vf7A30.79781000.527Contact Map0.565
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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