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OPENSEQ.org
PFLE - Putative pyruvate formate-lyase 3-activating enzyme
UniProt: P75794 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13470
Length: 299 (297)
Sequences: 1107
Seq/Len: 3.73
PFLE
Paralog alert: 0.50 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: NRDG PFLA PFLC PFLE YJJW
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
129_L 141_A 4.009 1.00
187_D 197_N 3.569 1.00
127_L 148_S 3.494 1.00
106_V 147_A 3.198 1.00
114_L 151_A 3.106 1.00
2_I 22_L 2.939 1.00
147_A 150_E 2.914 1.00
190_F 198_A 2.826 1.00
154_H 177_L 2.745 1.00
187_D 199_A 2.741 1.00
146_Q 174_Y 2.568 1.00
107_E 147_A 2.557 1.00
180_A 205_L 2.457 1.00
229_E 283_F 2.337 1.00
146_Q 170_P 2.272 1.00
149_H 176_D 2.243 1.00
189_P 192_Q 2.171 1.00
30_R 269_D 2.156 1.00
267_P 271_P 2.135 1.00
224_G 273_K 2.134 1.00
113_V 148_S 2.105 1.00
145_L 155_T 2.08 1.00
110_M 148_S 2.058 1.00
3_F 37_S 2.046 1.00
26_S 160_C 2.036 1.00
186_A 234_A 2.013 1.00
161_L 180_A 1.942 1.00
112_T 115_R 1.887 1.00
18_T 112_T 1.823 0.99
129_L 144_L 1.821 0.99
221_L 250_F 1.811 0.99
205_L 238_F 1.797 0.99
147_A 151_A 1.797 0.99
262_H 271_P 1.791 0.99
31_W 222_I 1.784 0.99
33_Q 158_E 1.783 0.99
276_D 281_L 1.766 0.99
280_L 283_F 1.764 0.99
85_L 88_L 1.73 0.99
237_D 291_K 1.62 0.98
190_F 201_V 1.615 0.98
189_P 231_S 1.612 0.98
285_Q 289_C 1.61 0.98
217_I 239_A 1.609 0.98
105_S 108_E 1.582 0.98
214_K 246_G 1.57 0.98
169_A 207_K 1.566 0.98
207_K 211_A 1.565 0.98
134_P 141_A 1.56 0.98
219_V 248_I 1.553 0.98
6_Q 9_S 1.546 0.98
185_V 238_F 1.542 0.98
104_K 109_I 1.539 0.98
199_A 203_D 1.529 0.98
229_E 233_K 1.511 0.97
157_V 178_F 1.486 0.97
228_D 231_S 1.482 0.97
239_A 248_I 1.48 0.97
227_A 280_L 1.43 0.96
282_D 285_Q 1.405 0.95
277_A 280_L 1.398 0.95
236_T 288_A 1.387 0.95
215_I 244_H 1.386 0.95
191_K 197_N 1.375 0.95
178_F 205_L 1.367 0.94
107_E 111_T 1.366 0.94
165_W 207_K 1.351 0.94
102_E 112_T 1.348 0.94
30_R 192_Q 1.331 0.93
198_A 201_V 1.324 0.93
158_E 216_I 1.304 0.92
221_L 281_L 1.302 0.92
229_E 280_L 1.301 0.92
233_K 287_Y 1.293 0.92
234_A 237_D 1.291 0.92
129_L 145_L 1.285 0.92
113_V 127_L 1.281 0.92
82_P 91_C 1.273 0.91
26_S 136_M 1.271 0.91
134_P 157_V 1.271 0.91
1_M 103_V 1.266 0.91
184_H 231_S 1.265 0.91
62_A 85_L 1.262 0.91
142_M 170_P 1.258 0.91
109_I 144_L 1.247 0.90
82_P 88_L 1.247 0.90
110_M 147_A 1.244 0.90
139_E 167_Y 1.238 0.90
82_P 85_L 1.23 0.89
188_A 191_K 1.227 0.89
34_N 260_K 1.215 0.88
282_D 286_Q 1.204 0.88
190_F 253_Y 1.198 0.88
230_T 234_A 1.195 0.87
221_L 284_A 1.194 0.87
145_L 175_I 1.193 0.87
193_W 225_F 1.186 0.87
206_K 238_F 1.181 0.86
38_R 266_L 1.18 0.86
149_H 174_Y 1.18 0.86
141_A 145_L 1.175 0.86
185_V 234_A 1.175 0.86
233_K 290_Q 1.161 0.85
161_L 204_N 1.16 0.85
114_L 117_K 1.158 0.85
27_L 201_V 1.157 0.85
104_K 108_E 1.156 0.85
109_I 127_L 1.151 0.85
173_P 211_A 1.15 0.84
109_I 113_V 1.14 0.84
170_P 173_P 1.139 0.84
169_A 211_A 1.126 0.83
219_V 235_I 1.12 0.82
62_A 88_L 1.119 0.82
158_E 179_L 1.117 0.82
82_P 89_T 1.116 0.82
82_P 86_T 1.116 0.82
278_P 282_D 1.114 0.82
106_V 144_L 1.103 0.81
232_V 284_A 1.096 0.80
216_I 247_E 1.093 0.80
27_L 196_G 1.091 0.80
184_H 189_P 1.085 0.79
5_I 18_T 1.084 0.79
202_L 238_F 1.082 0.79
14_P 177_L 1.077 0.79
233_K 291_K 1.076 0.79
135_F 171_S 1.073 0.78
55_C 58_C 1.07 0.78
86_T 89_T 1.056 0.77
40_R 136_M 1.055 0.77
66_I 82_P 1.049 0.76
185_V 231_S 1.045 0.76
3_F 36_E 1.042 0.75
117_K 121_D 1.035 0.75
31_W 220_P 1.031 0.74
18_T 116_D 1.025 0.74
114_L 148_S 1.022 0.73
250_F 281_L 1.019 0.73
87_A 90_D 1.013 0.73
35_P 261_Y 1.008 0.72
142_M 174_Y 1.008 0.72
20_V 109_I 1.005 0.72
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3c8fA10.79931000.611Contact Map0.777
3canA10.59871000.698Contact Map0.763
1hfeL20.86291000.703Contact Map0.137
3rfaA20.72241000.744Contact Map0.625
2yx0A10.77591000.764Contact Map0.679
2z2uA10.75921000.766Contact Map0.655
1q16B10.749299.90.785Contact Map0.124
3c8yA10.866299.90.787Contact Map0.146
1oltA10.779399.90.796Contact Map0.436
2vpzB20.548599.90.797Contact Map0.202
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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