OPENSEQ.org - PTMA - ECOLI GREMLIN

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PTMA - Mannitol-specific cryptic phosphotransferase enzyme IIA component

UniProt:	P69824	Sequence MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQAIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG11791
Length:	147 (145)
Sequences:	3513
Seq/Len:	24.23

PTMA
Paralog alert: 0.73 [within 20: 0.19] - ratio of genomes with paralogs
Cluster includes: PTFA PTKA PTMA PTSN SGCA ULAC

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
98_K	132_L	3.652	1.00
9_E	138_K	2.873	1.00
30_V	34_D	2.446	1.00
20_D	23_E	2.324	1.00
39_S	75_L	2.306	1.00
136_S	139_Q	2.303	1.00
129_E	133_T	2.235	1.00
26_D	43_I	2.131	1.00
28_S	102_G	2.074	1.00
15_I	83_L	2.04	1.00
17_S	85_E	2.035	1.00
84_L	100_L	2.02	1.00
29_M	43_I	1.993	1.00
22_Q	51_I	1.941	1.00
46_I	102_G	1.892	1.00
87_G	98_K	1.88	1.00
78_G	104_S	1.872	1.00
18_A	24_A	1.87	1.00
44_Q	48_D	1.863	1.00
18_A	27_F	1.847	1.00
37_Y	76_K	1.846	1.00
13_S	31_S	1.839	1.00
15_I	85_E	1.825	1.00
33_L	38_I	1.802	1.00
84_L	87_G	1.712	1.00
28_S	82_T	1.685	1.00
74_A	104_S	1.666	1.00
135_S	139_Q	1.665	1.00
24_A	100_L	1.658	1.00
16_H	82_T	1.6	1.00
11_S	35_K	1.554	1.00
50_T	57_Y	1.536	1.00
19_K	23_E	1.528	1.00
138_K	142_D	1.521	1.00
42_Y	104_S	1.438	1.00
10_S	35_K	1.427	1.00
41_N	73_G	1.398	0.99
13_S	27_F	1.39	0.99
37_Y	78_G	1.372	0.99
9_E	141_A	1.371	0.99
31_S	34_D	1.361	0.99
128_L	132_L	1.357	0.99
25_I	46_I	1.332	0.99
77_T	106_A	1.321	0.99
84_L	98_K	1.309	0.99
45_A	48_D	1.296	0.99
50_T	55_P	1.295	0.99
48_D	51_I	1.292	0.99
18_A	84_L	1.262	0.99
134_A	139_Q	1.259	0.99
134_A	140_L	1.236	0.98
29_M	102_G	1.231	0.98
83_L	99_L	1.208	0.98
97_I	100_L	1.206	0.98
14_V	83_L	1.191	0.98
61_P	128_L	1.182	0.98
26_D	47_K	1.181	0.98
12_I	140_L	1.176	0.98
22_Q	47_K	1.158	0.97
22_Q	26_D	1.157	0.97
48_D	52_N	1.14	0.97
29_M	46_I	1.128	0.97
111_H	115_I	1.114	0.96
134_A	143_I	1.107	0.96
62_G	98_K	1.102	0.96
16_H	27_F	1.084	0.96
81_L	99_L	1.081	0.95
23_E	26_D	1.079	0.95
82_T	102_G	1.064	0.95
33_L	40_E	1.051	0.94
9_E	137_E	1.05	0.94
71_E	107_D	1.044	0.94
8_P	11_S	1.026	0.94
116_Q	119_S	1.019	0.93
32_L	104_S	1.018	0.93
130_Q	133_T	1.013	0.93
31_S	35_K	1.004	0.93
89_Y	96_P	1.001	0.92

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
2oq3A	1	0.9932	100	0.041	Contact Map	0.684
3oxpA	2	0.9932	100	0.063	Contact Map	0.791
1a6jA	2	0.9796	100	0.064	Contact Map	0.738
3bjvA	1	0.9932	100	0.067	Contact Map	0.797
1a3aA	4	0.9796	100	0.069	Contact Map	0.78
2oqtA	4	0.9932	100	0.071	Contact Map	0.808
3lf6A	2	0.9932	100	0.074	Contact Map	0.709
2a0jA	1	0.9796	100	0.089	Contact Map	0.671
3urrA	2	0.9796	100	0.094	Contact Map	0.751
3t43A	2	0.8707	100	0.143	Contact Map	0.771

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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