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OPENSEQ.org
LCFA - Long-chain-fatty-acid--CoA ligase
UniProt: P69451 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11530
Length: 561 (511)
Sequences: 29670
Seq/Len: 58.06
LCFA
Paralog alert: 0.82 [within 20: 0.45] - ratio of genomes with paralogs
Cluster includes: ACSA CAIC ENTE FADK LCFA MENE PRPE
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
208_L 225_M 3.836 1.00
407_L 440_A 3.707 1.00
119_D 213_Y 3.38 1.00
71_L 99_M 3.231 1.00
388_E 410_K 3.178 1.00
59_R 96_R 2.961 1.00
421_R 424_A 2.845 1.00
75_D 123_S 2.829 1.00
78_A 122_A 2.818 1.00
441_V 449_R 2.765 1.00
227_T 416_L 2.696 1.00
459_L 464_N 2.691 1.00
393_D 397_N 2.688 1.00
477_H 516_F 2.647 1.00
55_E 96_R 2.547 1.00
482_E 538_K 2.537 1.00
121_G 420_Q 2.503 1.00
76_R 102_V 2.462 1.00
40_P 48_V 2.427 1.00
406_E 435_H 2.377 1.00
390_K 398_E 2.359 1.00
293_K 297_K 2.325 1.00
392_V 408_C 2.303 1.00
30_E 55_E 2.295 1.00
90_A 126_V 2.277 1.00
36_Y 39_Q 2.237 1.00
247_H 250_K 2.178 1.00
404_P 439_I 2.176 1.00
475_M 483_V 2.172 1.00
119_D 421_R 2.158 1.00
251_E 328_H 2.106 1.00
43_V 278_Q 2.106 1.00
422_P 426_D 2.095 1.00
57_R 89_V 2.08 1.00
474_V 499_I 2.067 1.00
424_A 427_E 2.047 1.00
94_I 99_M 2.046 1.00
379_S 449_R 2.024 1.00
252_L 278_Q 2.021 1.00
498_K 531_E 1.999 1.00
408_C 435_H 1.997 1.00
73_K 207_D 1.981 1.00
61_F 89_V 1.981 1.00
55_E 59_R 1.974 1.00
32_S 36_Y 1.962 1.00
545_L 548_E 1.948 1.00
64_Y 69_L 1.935 1.00
38_D 52_R 1.909 1.00
115_H 220_V 1.901 1.00
423_D 427_E 1.901 1.00
52_R 56_E 1.897 1.00
251_E 301_T 1.87 1.00
460_V 465_V 1.863 1.00
476_Q 520_Q 1.846 1.00
469_E 525_K 1.845 1.00
391_L 440_A 1.84 1.00
443_D 449_R 1.839 1.00
442_M 448_L 1.834 1.00
66_Q 97_A 1.833 1.00
390_K 408_C 1.832 1.00
123_S 145_Q 1.825 1.00
339_Q 343_E 1.789 1.00
72_K 75_D 1.775 1.00
114_E 118_N 1.737 1.00
61_F 93_G 1.724 1.00
280_L 300_F 1.719 1.00
77_V 94_I 1.711 1.00
50_T 53_K 1.701 1.00
49_M 89_V 1.697 1.00
44_N 85_L 1.68 1.00
479_G 512_S 1.676 1.00
440_A 450_I 1.674 1.00
58_S 96_R 1.67 1.00
95_L 209_A 1.669 1.00
443_D 447_F 1.658 1.00
456_D 546_R 1.657 1.00
509_T 512_S 1.657 1.00
78_A 104_V 1.656 1.00
204_V 207_D 1.655 1.00
31_Q 35_R 1.65 1.00
548_E 551_D 1.649 1.00
299_P 326_S 1.633 1.00
343_E 347_K 1.631 1.00
93_G 97_A 1.628 1.00
59_R 63_A 1.621 1.00
64_Y 189_A 1.605 1.00
485_A 499_I 1.605 1.00
71_L 77_V 1.596 1.00
43_V 48_V 1.592 1.00
305_G 310_F 1.591 1.00
227_T 230_N 1.59 1.00
77_V 99_M 1.585 1.00
404_P 451_V 1.575 1.00
500_F 549_L 1.567 1.00
120_S 213_Y 1.565 1.00
41_A 54_L 1.565 1.00
74_G 207_D 1.551 1.00
441_V 451_V 1.55 1.00
502_V 536_L 1.548 1.00
78_A 117_L 1.546 1.00
39_Q 250_K 1.541 1.00
43_V 298_Y 1.532 1.00
208_L 416_L 1.532 1.00
401_P 442_M 1.53 1.00
295_L 326_S 1.526 1.00
376_H 445_E 1.523 1.00
225_M 417_G 1.515 1.00
211_L 419_W 1.514 1.00
261_H 264_A 1.514 1.00
389_A 409_V 1.491 1.00
471_E 485_A 1.489 1.00
475_M 482_E 1.488 1.00
76_R 419_W 1.488 1.00
88_P 269_C 1.488 1.00
82_P 128_V 1.476 1.00
230_N 414_V 1.473 1.00
400_P 403_Q 1.462 1.00
37_A 52_R 1.461 1.00
539_S 545_L 1.457 1.00
518_R 526_V 1.455 1.00
486_V 500_F 1.448 1.00
56_E 60_A 1.444 1.00
53_K 56_E 1.442 1.00
230_N 363_A 1.441 1.00
393_D 399_V 1.44 1.00
359_L 434_L 1.434 1.00
213_Y 223_G 1.42 1.00
58_S 93_G 1.417 1.00
40_P 50_T 1.409 1.00
119_D 420_Q 1.408 1.00
407_L 450_I 1.407 0.99
40_P 43_V 1.401 0.99
104_V 113_L 1.395 0.99
310_F 330_S 1.395 0.99
32_S 35_R 1.395 0.99
290_G 293_K 1.394 0.99
48_V 298_Y 1.393 0.99
272_F 277_G 1.392 0.99
206_E 229_R 1.39 0.99
332_G 337_V 1.388 0.99
33_V 55_E 1.386 0.99
511_E 515_T 1.385 0.99
516_F 520_Q 1.381 0.99
71_L 124_A 1.38 0.99
254_V 300_F 1.377 0.99
514_V 530_V 1.377 0.99
49_M 85_L 1.374 0.99
247_H 251_E 1.357 0.99
251_E 302_A 1.35 0.99
458_I 467_P 1.349 0.99
61_F 126_V 1.348 0.99
430_K 435_H 1.344 0.99
428_I 434_L 1.339 0.99
100_I 208_L 1.337 0.99
515_T 519_R 1.325 0.99
40_P 278_Q 1.322 0.99
410_K 433_W 1.321 0.99
409_V 436_T 1.314 0.99
30_E 96_R 1.308 0.99
404_P 441_V 1.297 0.99
74_G 416_L 1.296 0.99
287_D 290_G 1.295 0.99
308_T 335_M 1.29 0.99
296_A 322_L 1.29 0.99
57_R 60_A 1.281 0.99
252_L 300_F 1.276 0.99
185_S 188_S 1.273 0.99
54_L 92_F 1.267 0.99
214_T 224_A 1.265 0.99
226_L 230_N 1.247 0.99
439_I 454_K 1.244 0.98
118_N 220_V 1.242 0.98
117_L 122_A 1.242 0.98
79_L 90_A 1.239 0.98
490_S 496_A 1.238 0.98
415_M 429_I 1.236 0.98
376_H 447_F 1.232 0.98
29_F 33_V 1.232 0.98
488_V 498_K 1.228 0.98
473_V 520_Q 1.224 0.98
394_D 454_K 1.222 0.98
366_V 409_V 1.215 0.98
282_I 294_E 1.215 0.98
468_N 472_D 1.211 0.98
338_Q 341_V 1.192 0.98
522_T 525_K 1.191 0.98
76_R 100_I 1.189 0.98
411_G 414_V 1.188 0.98
510_E 532_F 1.188 0.98
114_E 141_K 1.186 0.98
71_L 75_D 1.185 0.98
473_V 517_C 1.181 0.98
391_L 399_V 1.18 0.98
406_E 454_K 1.176 0.98
115_H 119_D 1.175 0.98
102_V 120_S 1.175 0.98
225_M 419_W 1.172 0.98
261_H 265_L 1.171 0.98
294_E 298_Y 1.169 0.97
383_P 389_A 1.168 0.97
513_L 530_V 1.168 0.97
92_F 273_I 1.168 0.97
303_I 327_L 1.164 0.97
112_E 525_K 1.16 0.97
211_L 223_G 1.158 0.97
337_V 342_A 1.158 0.97
76_R 211_L 1.152 0.97
479_G 506_P 1.146 0.97
488_V 529_L 1.145 0.97
399_V 403_Q 1.143 0.97
457_M 466_Y 1.139 0.97
517_C 526_V 1.134 0.97
500_F 550_R 1.133 0.97
407_L 448_L 1.133 0.97
389_A 407_L 1.132 0.97
516_F 519_R 1.129 0.97
115_H 213_Y 1.129 0.97
498_K 529_L 1.129 0.97
518_R 523_G 1.128 0.97
382_L 447_F 1.125 0.97
252_L 280_L 1.124 0.97
501_V 530_V 1.121 0.97
501_V 532_F 1.12 0.96
54_L 89_V 1.115 0.96
49_M 57_R 1.113 0.96
239_N 275_L 1.111 0.96
387_T 413_Q 1.111 0.96
41_A 51_F 1.11 0.96
458_I 497_V 1.11 0.96
461_S 493_S 1.106 0.96
489_P 495_E 1.104 0.96
456_D 468_N 1.103 0.96
367_S 389_A 1.098 0.96
24_S 27_D 1.098 0.96
389_A 448_L 1.095 0.96
262_I 265_L 1.091 0.96
380_I 450_I 1.091 0.96
215_G 541_V 1.086 0.96
474_V 483_V 1.085 0.96
119_D 220_V 1.084 0.96
272_F 279_N 1.083 0.96
23_Q 31_Q 1.083 0.96
510_E 530_V 1.075 0.95
212_Q 360_T 1.075 0.95
55_E 58_S 1.074 0.95
476_Q 516_F 1.072 0.95
500_F 533_R 1.071 0.95
439_I 452_D 1.07 0.95
49_M 54_L 1.068 0.95
61_F 90_A 1.065 0.95
258_P 261_H 1.063 0.95
54_L 58_S 1.06 0.95
64_Y 67_Q 1.06 0.95
477_H 512_S 1.059 0.95
344_R 348_L 1.058 0.95
393_D 403_Q 1.057 0.95
57_R 86_Q 1.053 0.95
47_E 57_R 1.051 0.94
477_H 513_L 1.038 0.94
390_K 433_W 1.037 0.94
85_L 89_V 1.036 0.94
362_C 434_L 1.035 0.94
65_L 126_V 1.034 0.94
261_H 266_T 1.03 0.94
224_A 415_M 1.029 0.94
499_I 530_V 1.02 0.93
205_P 229_R 1.018 0.93
229_R 412_P 1.015 0.93
315_N 344_R 1.014 0.93
39_Q 278_Q 1.014 0.93
536_L 544_I 1.013 0.93
391_L 448_L 1.011 0.93
280_L 294_E 1.01 0.93
463_F 524_Y 1.006 0.93
302_A 329_L 1.004 0.93
62_A 96_R 1.003 0.93
209_A 228_H 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3etcA20.9341000.2Contact Map0.844
3tsyA10.94831000.203Contact Map0.777
1mdbA10.9341000.206Contact Map0.765
3o83A20.93941000.208Contact Map0.748
3r44A10.91441000.213Contact Map0.809
3g7sA20.92691000.214Contact Map0.803
3ni2A10.92341000.215Contact Map0.802
2d1sA10.93231000.22Contact Map0.714
1pg4A20.95721000.222Contact Map0.773
1v25A20.92691000.224Contact Map0.751
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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