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OPENSEQ.org
ISPG - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
UniProt: P62620 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10370
Length: 372 (353)
Sequences: 1017
Seq/Len: 2.88
ISPG
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
193_R 230_E 5.614 1.00
58_I 84_V 3.616 1.00
76_K 103_D 3.462 1.00
16_Y 21_P 3.136 1.00
144_D 163_R 2.989 1.00
96_K 99_E 2.797 1.00
14_R 21_P 2.748 1.00
12_S 196_A 2.733 1.00
14_R 26_A 2.6 1.00
165_V 178_V 2.508 1.00
41_V 45_V 2.443 1.00
155_Q 194_L 2.436 1.00
32_S 51_L 2.39 1.00
279_D 282_G 2.342 1.00
74_L 77_Q 2.342 1.00
188_A 207_I 2.333 1.00
36_T 44_T 2.266 1.00
52_E 80_N 2.248 1.00
14_R 25_G 2.24 1.00
46_N 50_A 2.223 1.00
42_E 78_Q 2.203 1.00
45_V 79_V 2.186 1.00
238_V 254_I 2.131 1.00
216_G 240_L 2.13 1.00
13_T 174_D 2.106 1.00
45_V 78_Q 2.081 1.00
14_R 24_D 2.065 1.00
79_V 83_L 2.064 1.00
50_A 245_V 2.048 1.00
10_R 196_A 2.038 1.00
115_E 170_R 2.031 1.00
104_C 177_K 2.023 1.00
189_V 226_L 1.993 1.00
343_D 347_Q 1.979 1.00
13_T 200_D 1.945 0.99
120_M 123_D 1.944 0.99
11_K 25_G 1.906 0.99
16_Y 19_N 1.898 0.99
76_K 83_L 1.87 0.99
129_N 175_Q 1.869 0.99
40_D 43_A 1.856 0.99
217_A 250_V 1.849 0.99
166_D 170_R 1.848 0.99
39_T 65_T 1.816 0.99
50_A 243_D 1.798 0.99
15_I 177_K 1.793 0.99
347_Q 350_A 1.771 0.99
34_T 44_T 1.766 0.99
207_I 223_G 1.75 0.99
291_L 300_V 1.735 0.98
142_E 160_S 1.718 0.98
84_V 104_C 1.695 0.98
289_Q 292_E 1.682 0.98
126_R 172_N 1.671 0.98
45_V 80_N 1.667 0.98
73_K 100_Y 1.662 0.98
42_E 46_N 1.662 0.98
138_A 182_A 1.658 0.98
169_D 201_Q 1.639 0.98
122_V 171_L 1.621 0.97
350_A 354_A 1.618 0.97
52_E 79_V 1.609 0.97
351_R 354_A 1.594 0.97
49_K 80_N 1.575 0.97
70_E 96_K 1.56 0.96
29_A 57_D 1.543 0.96
241_A 269_A 1.54 0.96
41_V 71_A 1.538 0.96
21_P 26_A 1.536 0.96
326_K 338_R 1.53 0.96
205_L 236_L 1.514 0.96
246_E 249_K 1.509 0.96
30_V 251_G 1.505 0.95
41_V 74_L 1.492 0.95
282_G 286_A 1.475 0.95
337_D 347_Q 1.457 0.94
290_R 349_E 1.452 0.94
222_I 254_I 1.448 0.94
52_E 81_V 1.446 0.94
99_E 128_K 1.444 0.94
169_D 174_D 1.432 0.94
28_I 57_D 1.428 0.93
98_A 125_A 1.42 0.93
62_S 274_S 1.394 0.92
142_E 163_R 1.393 0.92
119_R 171_L 1.389 0.92
22_I 58_I 1.387 0.92
20_V 29_A 1.381 0.92
343_D 346_D 1.378 0.92
180_V 195_L 1.373 0.92
138_A 141_L 1.368 0.91
38_T 44_T 1.362 0.91
84_V 177_K 1.362 0.91
340_D 343_D 1.356 0.91
123_D 127_D 1.353 0.91
318_L 355_K 1.352 0.91
167_H 171_L 1.347 0.91
145_L 156_A 1.335 0.90
178_V 195_L 1.332 0.90
325_K 341_N 1.332 0.90
96_K 100_Y 1.33 0.90
36_T 47_Q 1.325 0.90
226_L 230_E 1.319 0.89
122_V 168_L 1.314 0.89
266_N 299_D 1.313 0.89
224_L 238_V 1.301 0.88
331_E 336_K 1.298 0.88
221_A 225_G 1.297 0.88
166_D 169_D 1.294 0.88
290_R 346_D 1.291 0.88
178_V 199_I 1.291 0.88
242_A 246_E 1.263 0.86
49_K 52_E 1.262 0.86
29_A 251_G 1.254 0.86
95_L 102_V 1.253 0.86
327_S 344_M 1.243 0.85
194_L 198_Q 1.239 0.85
286_A 290_R 1.237 0.85
155_Q 190_E 1.226 0.84
221_A 254_I 1.224 0.84
278_F 322_G 1.223 0.84
73_K 77_Q 1.217 0.83
72_F 97_V 1.204 0.83
277_E 324_N 1.199 0.82
115_E 119_R 1.194 0.82
155_Q 198_Q 1.188 0.81
159_E 163_R 1.177 0.81
29_A 58_I 1.162 0.79
66_M 70_E 1.157 0.79
164_H 168_L 1.153 0.79
148_K 159_E 1.15 0.78
30_V 252_F 1.137 0.77
34_T 38_T 1.135 0.77
241_A 280_V 1.131 0.77
222_I 225_G 1.125 0.76
21_P 25_G 1.122 0.76
329_L 348_L 1.121 0.76
35_N 276_Q 1.117 0.75
162_M 199_I 1.115 0.75
98_A 130_I 1.114 0.75
157_L 180_V 1.112 0.75
22_I 177_K 1.108 0.75
166_D 201_Q 1.107 0.75
49_K 53_R 1.106 0.74
162_M 198_Q 1.101 0.74
337_D 351_R 1.098 0.74
21_P 29_A 1.097 0.74
214_R 253_D 1.089 0.73
44_T 61_V 1.085 0.72
56_A 248_I 1.084 0.72
176_F 201_Q 1.083 0.72
165_V 180_V 1.082 0.72
124_C 128_K 1.076 0.72
195_L 199_I 1.071 0.71
99_E 124_C 1.069 0.71
298_M 317_T 1.067 0.71
89_F 306_V 1.067 0.71
44_T 47_Q 1.067 0.71
45_V 83_L 1.066 0.71
49_K 78_Q 1.065 0.70
293_D 353_R 1.064 0.70
74_L 78_Q 1.061 0.70
226_L 229_S 1.061 0.70
145_L 163_R 1.047 0.69
301_S 316_S 1.047 0.69
37_R 68_A 1.045 0.68
49_K 79_V 1.041 0.68
132_I 173_F 1.035 0.67
54_V 245_V 1.032 0.67
64_P 125_A 1.032 0.67
153_T 156_A 1.031 0.67
328_G 338_R 1.026 0.66
348_L 352_I 1.025 0.66
349_E 353_R 1.025 0.66
72_F 76_K 1.023 0.66
61_V 79_V 1.021 0.66
22_I 29_A 1.021 0.66
283_T 345_I 1.018 0.65
38_T 47_Q 1.018 0.65
57_D 261_R 1.017 0.65
119_R 123_D 1.014 0.65
98_A 124_C 1.006 0.64
286_A 289_Q 1.003 0.64
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4g9pA10.96771000.293Contact Map0.625
3noyA40.97311000.332Contact Map0.725
4mwaA80.73661000.517Contact Map0.766
2yr1A20.60221000.739Contact Map0.406
1zcoA20.612999.90.852Contact Map0.464
4g38A10.959799.80.88Contact Map0.299
1zj8A20.948999.70.891Contact Map0.382
1tx2A20.661398.40.942Contact Map0.52
1h5yA20.634498.20.944Contact Map0.358
4djdD20.6344980.948Contact Map0.458
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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