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OPENSEQ.org

ISPF - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
UniProt: P62617 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11816
Length: 159 (157)
Sequences: 1412
Seq/Len: 8.99

ISPF
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
17_P 24_R 3.345 1.00
33_A 37_G 2.939 1.00
74_R 119_D 2.864 1.00
79_E 82_R 2.611 1.00
44_L 94_V 2.484 1.00
41_L 116_I 2.337 1.00
45_T 80_A 2.292 1.00
40_A 96_V 2.27 1.00
13_G 32_L 2.222 1.00
21_G 40_A 2.209 1.00
9_V 137_L 2.178 1.00
48_L 84_I 2.16 1.00
99_I 149_E 2.08 1.00
44_L 96_V 2.06 1.00
27_Y 143_G 1.958 1.00
98_I 105_M 1.926 1.00
122_C 126_D 1.918 1.00
5_H 149_E 1.906 1.00
79_E 83_R 1.821 1.00
134_T 140_T 1.798 1.00
102_A 143_G 1.748 1.00
87_K 125_D 1.74 1.00
3_I 153_L 1.724 1.00
31_L 37_G 1.61 1.00
59_K 109_I 1.608 1.00
92_G 155_I 1.577 1.00
52_A 84_I 1.488 1.00
5_H 97_T 1.482 1.00
71_A 76_L 1.476 1.00
54_L 87_K 1.464 1.00
100_A 146_I 1.446 0.99
81_W 120_L 1.415 0.99
84_I 154_L 1.414 0.99
140_T 147_A 1.403 0.99
68_F 71_A 1.384 0.99
11_A 32_L 1.383 0.99
41_L 45_T 1.382 0.99
104_K 107_P 1.376 0.99
91_L 122_C 1.369 0.99
108_H 111_Q 1.361 0.99
5_H 151_V 1.353 0.99
115_F 118_E 1.342 0.99
85_Q 121_G 1.337 0.99
114_V 118_E 1.336 0.99
74_R 115_F 1.331 0.99
20_I 105_M 1.33 0.99
111_Q 114_V 1.327 0.99
114_V 124_M 1.317 0.99
81_W 91_L 1.289 0.99
7_F 134_T 1.284 0.99
93_N 153_L 1.283 0.99
66_P 69_K 1.278 0.98
21_G 112_M 1.272 0.98
85_Q 90_T 1.265 0.98
82_R 86_A 1.26 0.98
75_E 78_R 1.245 0.98
51_A 154_L 1.243 0.98
113_R 124_M 1.227 0.98
63_D 101_Q 1.209 0.98
99_I 140_T 1.197 0.97
78_R 119_D 1.194 0.97
90_T 155_I 1.178 0.97
123_H 126_D 1.178 0.97
139_F 145_G 1.163 0.97
44_L 116_I 1.161 0.97
101_Q 136_K 1.149 0.96
20_I 39_V 1.148 0.96
45_T 76_L 1.142 0.96
60_L 83_R 1.138 0.96
55_G 59_K 1.131 0.96
53_A 128_N 1.12 0.96
91_L 94_V 1.114 0.96
110_P 114_V 1.109 0.95
106_L 110_P 1.097 0.95
81_W 85_Q 1.091 0.95
89_Y 156_K 1.091 0.95
17_P 30_G 1.088 0.95
47_A 150_A 1.082 0.95
50_G 95_D 1.081 0.94
92_G 153_L 1.08 0.94
52_A 154_L 1.079 0.94
138_G 145_G 1.072 0.94
9_V 134_T 1.07 0.94
19_I 24_R 1.065 0.94
9_V 139_F 1.061 0.94
95_D 130_K 1.052 0.93
31_L 100_A 1.03 0.92
68_F 76_L 1.001 0.91
110_P 113_R 1 0.91
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1gx1A311000.018Contact Map0.875
3f0dA611000.021Contact Map0.841
1t0aA30.98741000.023Contact Map0.883
2pmpA10.98741000.024Contact Map0.695
3re3A40.98111000.025Contact Map0.812
2uzhA30.98111000.048Contact Map0.735
3b6nA10.99371000.054Contact Map0.696
1iv3A60.9561000.062Contact Map0.84
1w55A111000.107Contact Map0.674
2wkbA60.43442.10.947Contact Map0.387

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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