OPENSEQ.org - FLIJ - ECOLI GREMLIN

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FLIJ - Flagellar FliJ protein

UniProt:	P52613	Sequence MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAGITSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG20267
Length:	147 (146)
Sequences:	323
Seq/Len:	2.21

FLIJ
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
116_E	120_T	2.262	1.00
46_Q	77_E	2.232	1.00
18_V	108_L	2.046	0.99
28_M	97_A	1.923	0.99
37_E	99_N	1.768	0.98
83_H	87_L	1.611	0.95
49_Y	101_W	1.538	0.94
3_E	6_A	1.53	0.93
21_A	25_L	1.525	0.93
7_L	114_L	1.378	0.88
10_L	68_N	1.351	0.86
2_A	145_K	1.337	0.85
11_K	115_Q	1.334	0.85
32_C	116_E	1.333	0.85
42_L	79_A	1.329	0.85
32_C	94_V	1.325	0.85
43_I	73_I	1.299	0.83
73_I	89_Q	1.299	0.83
132_K	137_F	1.292	0.83
31_G	34_Q	1.277	0.82
29_R	69_Y	1.27	0.81
10_L	19_E	1.268	0.81
107_R	139_Q	1.265	0.81
48_E	84_R	1.264	0.81
88_N	92_Q	1.259	0.80
132_K	136_E	1.259	0.80
73_I	130_D	1.246	0.79
26_G	73_I	1.224	0.78
69_Y	77_E	1.223	0.77
81_T	120_T	1.204	0.76
70_Q	140_R	1.203	0.76
101_W	105_K	1.196	0.75
41_M	68_N	1.194	0.75
33_Q	99_N	1.194	0.75
46_Q	130_D	1.193	0.75
109_Q	112_Q	1.191	0.75
28_M	138_A	1.183	0.74
85_Q	88_N	1.183	0.74
10_L	114_L	1.168	0.73
59_A	99_N	1.165	0.73
76_L	110_A	1.161	0.72
12_D	16_K	1.155	0.72
5_G	115_Q	1.15	0.71
10_L	111_W	1.146	0.71
27_E	30_R	1.146	0.71
63_S	66_W	1.145	0.71
14_A	72_F	1.142	0.70
46_Q	49_Y	1.139	0.70
80_I	130_D	1.134	0.70
106_Q	110_A	1.133	0.69
17_E	114_L	1.126	0.69
68_N	140_R	1.107	0.67
39_L	80_I	1.09	0.65
10_L	113_T	1.083	0.64
45_Y	68_N	1.081	0.64
19_E	105_K	1.08	0.64
11_K	83_H	1.078	0.64
2_A	126_E	1.068	0.63
57_M	60_G	1.066	0.63
94_V	109_Q	1.065	0.63
95_D	99_N	1.064	0.62
112_Q	116_E	1.059	0.62
32_C	87_L	1.053	0.61
75_T	118_Q	1.051	0.61
88_N	91_T	1.045	0.60
29_R	98_L	1.035	0.59
45_Y	72_F	1.03	0.59
40_K	88_N	1.029	0.59
98_L	129_L	1.028	0.58
24_L	112_Q	1.023	0.58
49_Y	72_F	1.022	0.58
38_Q	75_T	1.021	0.58
44_D	114_L	1.017	0.57
129_L	133_K	1.017	0.57
45_Y	128_R	1.013	0.57
97_A	106_Q	1.005	0.56
52_N	56_D	1.004	0.56
87_L	90_W	1.001	0.55
1_M	33_Q	1	0.55

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
3ajwA	1	1	100	0.194	Contact Map	0.354
3k29A	1	0.9932	99.9	0.349	Contact Map	0.334
4mh6A	1	0.9728	96.2	0.796	Contact Map	0.262
4dndA	1	0.585	10	0.921	Contact Map	0.29
3si5X	2	0.1633	3.8	0.935	Contact Map	0.207
3nr7A	2	0.4422	3.4	0.936	Contact Map	0.517
1i84S	2	0.8776	2.5	0.94	Contact Map
3tnuA	1	0.7891	2.3	0.942	Contact Map	0.249
1t3jA	1	0.3469	2.2	0.942	Contact Map	0.499
3mq9A	8	0.6122	2.2	0.942	Contact Map	0.262

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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