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OPENSEQ.org
TILS - tRNA(Ile)-lysidine synthase
UniProt: P52097 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13220
Length: 432 (417)
Sequences: 1356
Seq/Len: 3.25
TILS
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
26_T 67_V 3.972 1.00
56_N 178_E 3.927 1.00
50_H 58_D 3.655 1.00
27_V 167_A 3.572 1.00
160_R 164_V 3.147 1.00
47_I 100_A 3.144 1.00
183_D 192_L 3.037 1.00
19_F 46_A 2.821 1.00
121_C 209_T 2.791 1.00
46_A 68_C 2.79 1.00
50_H 57_A 2.76 1.00
46_A 73_V 2.729 1.00
183_D 193_R 2.709 1.00
19_F 64_C 2.52 1.00
50_H 61_V 2.52 1.00
23_L 167_A 2.465 1.00
23_L 27_V 2.44 1.00
29_L 46_A 2.438 1.00
375_R 383_E 2.423 1.00
45_R 76_V 2.421 1.00
29_L 68_C 2.419 1.00
105_L 111_L 2.387 1.00
60_W 175_I 2.354 1.00
199_Q 206_A 2.318 1.00
377_L 381_W 2.29 1.00
118_D 206_A 2.266 1.00
78_E 103_R 2.26 1.00
15_I 112_V 2.252 1.00
176_E 181_Q 2.213 1.00
54_S 178_E 2.202 1.00
68_C 75_L 2.188 1.00
61_V 65_E 2.172 1.00
30_H 166_W 2.143 1.00
65_E 69_Q 2.136 1.00
18_A 101_F 2.133 1.00
126_L 186_D 2.108 1.00
61_V 77_V 2.1 1.00
17_V 32_L 2.083 1.00
112_V 152_I 2.054 1.00
30_H 170_Y 2.043 1.00
23_L 163_L 2.005 1.00
218_E 255_A 1.969 1.00
26_T 172_L 1.919 1.00
19_F 26_T 1.877 0.99
30_H 71_W 1.871 0.99
398_F 403_L 1.87 0.99
124_F 213_A 1.863 0.99
113_T 151_L 1.842 0.99
366_L 374_G 1.8 0.99
358_V 397_L 1.785 0.99
52_G 79_R 1.77 0.99
19_F 67_V 1.76 0.99
31_Q 155_L 1.758 0.99
230_D 253_R 1.752 0.99
16_L 45_R 1.745 0.99
16_L 104_T 1.73 0.99
254_R 279_W 1.726 0.99
190_L 194_V 1.724 0.99
91_A 95_Q 1.715 0.99
120_Q 140_M 1.708 0.99
134_P 216_C 1.675 0.98
53_L 83_A 1.672 0.98
53_L 89_I 1.668 0.98
90_E 179_S 1.66 0.98
33_V 73_V 1.647 0.98
219_Q 256_A 1.639 0.98
361_K 392_D 1.629 0.98
67_V 71_W 1.618 0.98
61_V 79_R 1.597 0.98
378_K 382_Q 1.591 0.98
189_F 201_R 1.585 0.98
17_V 28_L 1.573 0.97
396_L 403_L 1.569 0.97
98_Y 113_T 1.562 0.97
367_H 380_I 1.556 0.97
225_E 250_S 1.553 0.97
133_G 136_G 1.534 0.97
167_A 172_L 1.527 0.97
121_C 195_V 1.523 0.97
212_S 216_C 1.52 0.97
23_L 176_E 1.52 0.97
30_H 172_L 1.518 0.97
141_A 144_S 1.517 0.97
128_L 212_S 1.516 0.97
165_Q 169_Q 1.509 0.96
26_T 63_H 1.508 0.96
398_F 401_E 1.507 0.96
132_S 136_G 1.503 0.96
413_Q 416_V 1.496 0.96
14_Q 43_A 1.494 0.96
381_W 394_T 1.484 0.96
15_I 152_I 1.469 0.96
13_R 109_E 1.456 0.95
121_C 206_A 1.451 0.95
13_R 110_V 1.448 0.95
227_L 257_I 1.447 0.95
47_I 104_T 1.446 0.95
49_V 100_A 1.446 0.95
277_R 281_E 1.431 0.95
62_T 65_E 1.428 0.95
159_T 162_E 1.4 0.94
124_F 209_T 1.387 0.94
49_V 78_E 1.376 0.93
242_Q 245_P 1.365 0.93
121_C 213_A 1.363 0.93
208_A 211_R 1.358 0.93
82_L 92_Q 1.356 0.93
46_A 64_C 1.355 0.93
129_K 209_T 1.354 0.92
59_A 175_I 1.354 0.92
381_W 406_A 1.344 0.92
129_K 198_L 1.342 0.92
379_K 383_E 1.331 0.92
411_V 416_V 1.33 0.92
47_I 101_F 1.328 0.92
68_C 73_V 1.324 0.91
361_K 377_L 1.323 0.91
89_I 185_Y 1.321 0.91
379_K 382_Q 1.32 0.91
54_S 60_W 1.313 0.91
32_L 112_V 1.304 0.91
137_L 212_S 1.294 0.90
17_V 46_A 1.29 0.90
19_F 63_H 1.274 0.89
117_L 210_A 1.265 0.89
113_T 140_M 1.263 0.89
129_K 202_W 1.263 0.89
49_V 80_V 1.259 0.88
359_R 365_L 1.258 0.88
135_A 220_E 1.258 0.88
215_L 272_R 1.25 0.88
124_F 128_L 1.249 0.88
215_L 219_Q 1.246 0.88
167_A 174_W 1.235 0.87
124_F 139_A 1.227 0.87
30_H 34_Q 1.218 0.86
60_W 64_C 1.212 0.86
65_E 77_V 1.203 0.85
253_R 256_A 1.202 0.85
121_C 210_A 1.201 0.85
222_L 226_L 1.2 0.85
258_I 278_I 1.2 0.85
361_K 365_L 1.191 0.84
58_D 79_R 1.19 0.84
26_T 174_W 1.186 0.84
90_E 185_Y 1.169 0.83
158_R 163_L 1.168 0.83
161_G 199_Q 1.162 0.82
226_L 229_D 1.161 0.82
95_Q 99_Q 1.161 0.82
361_K 378_K 1.149 0.81
14_Q 45_R 1.149 0.81
27_V 166_W 1.149 0.81
31_Q 158_R 1.137 0.80
166_W 172_L 1.134 0.80
32_L 44_L 1.119 0.79
249_M 257_I 1.118 0.79
48_H 60_W 1.117 0.79
159_T 206_A 1.114 0.78
138_S 141_A 1.112 0.78
45_R 108_G 1.11 0.78
134_P 219_Q 1.109 0.78
22_G 25_S 1.107 0.78
228_A 260_R 1.102 0.77
227_L 256_A 1.099 0.77
35_W 42_V 1.098 0.77
20_S 25_S 1.095 0.77
101_F 105_L 1.094 0.77
412_T 415_G 1.09 0.76
146_F 149_T 1.09 0.76
204_H 208_A 1.088 0.76
360_F 395_P 1.082 0.76
23_L 164_V 1.082 0.76
193_R 197_L 1.074 0.75
179_S 188_N 1.074 0.75
82_L 88_G 1.071 0.74
20_S 24_D 1.069 0.74
195_V 209_T 1.064 0.74
155_L 158_R 1.06 0.73
62_T 66_N 1.054 0.73
80_V 96_A 1.054 0.73
382_Q 385_G 1.05 0.72
361_K 364_G 1.049 0.72
348_R 352_A 1.048 0.72
16_L 108_G 1.041 0.71
50_H 178_E 1.041 0.71
205_F 209_T 1.038 0.71
356_V 404_I 1.037 0.71
57_A 178_E 1.032 0.71
65_E 75_L 1.028 0.70
333_P 337_G 1.028 0.70
96_A 99_Q 1.026 0.70
292_C 299_E 1.023 0.70
376_K 379_K 1.021 0.69
120_Q 156_L 1.021 0.69
134_P 215_L 1.018 0.69
59_A 62_T 1.014 0.69
230_D 256_A 1.012 0.68
52_G 58_D 1.011 0.68
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1ni5A10.99541000.209Contact Map0.65
3a2kA20.98841000.221Contact Map0.621
1wy5A20.68751000.458Contact Map0.795
3vrhA10.59721000.641Contact Map0.63
3rjzA10.52311000.745Contact Map0.417
3uowA20.59951000.755Contact Map0.482
1zunA10.58331000.757Contact Map0.587
2dplA20.61811000.76Contact Map0.604
3k32A60.44441000.765Contact Map0.653
2vxoA20.68521000.767Contact Map0.532
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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