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OPENSEQ.org
GLCF - Glycolate oxidase iron-sulfur subunit
UniProt: P52074 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13291
Length: 407 (389)
Sequences: 2680
Seq/Len: 6.89
GLCF
Paralog alert: 0.62 [within 20: 0.02] - ratio of genomes with paralogs
Cluster includes: GLCF GLPC YKGE
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
188_A 398_E 3.821 1.00
301_K 330_T 3.811 1.00
394_R 402_Q 3.785 1.00
191_R 401_E 3.393 1.00
169_R 199_S 3.008 1.00
208_C 251_C 2.823 1.00
282_L 397_I 2.731 1.00
189_T 247_T 2.592 1.00
176_C 208_C 2.586 1.00
301_K 332_T 2.529 1.00
256_K 284_E 2.49 1.00
227_R 231_D 2.433 1.00
303_A 334_V 2.37 1.00
335_P 368_S 2.367 1.00
321_E 333_D 2.346 1.00
302_L 399_I 2.331 1.00
209_C 251_C 2.27 1.00
306_C 321_E 2.263 1.00
334_V 366_L 2.247 1.00
198_I 285_L 2.2 1.00
178_Q 186_N 2.2 1.00
234_W 271_K 2.18 1.00
218_A 221_K 2.153 1.00
29_G 49_R 2.151 1.00
169_R 242_E 2.129 1.00
90_R 93_N 2.095 1.00
231_D 268_Y 2.086 1.00
189_T 282_L 2.033 1.00
184_N 398_E 2.01 1.00
172_M 247_T 1.957 1.00
82_E 90_R 1.927 1.00
185_T 398_E 1.914 1.00
375_V 399_I 1.906 1.00
226_A 258_Y 1.902 1.00
176_C 209_C 1.896 1.00
192_V 404_L 1.875 1.00
196_L 404_L 1.872 1.00
192_V 400_V 1.834 1.00
352_P 356_R 1.795 1.00
190_A 200_V 1.782 1.00
170_V 200_V 1.742 1.00
211_A 250_G 1.732 1.00
245_L 285_L 1.722 1.00
246_Q 252_G 1.693 1.00
229_N 258_Y 1.679 1.00
176_C 251_C 1.674 1.00
168_R 198_I 1.659 1.00
307_P 376_T 1.642 1.00
280_V 284_E 1.623 1.00
170_V 285_L 1.607 1.00
193_L 245_L 1.594 1.00
360_D 364_N 1.592 1.00
223_L 227_R 1.57 1.00
44_E 49_R 1.552 0.99
366_L 374_I 1.548 0.99
207_G 228_N 1.543 0.99
238_E 274_Q 1.538 0.99
248_A 378_N 1.527 0.99
311_Q 317_N 1.522 0.99
125_R 128_V 1.521 0.99
189_T 193_L 1.519 0.99
302_L 329_F 1.517 0.99
306_C 333_D 1.511 0.99
322_K 325_L 1.508 0.99
100_D 219_Q 1.473 0.99
366_L 385_L 1.472 0.99
161_R 187_A 1.469 0.99
302_L 375_V 1.461 0.99
394_R 398_E 1.453 0.99
300_K 372_E 1.439 0.99
273_R 277_E 1.433 0.99
304_F 320_V 1.427 0.99
175_G 248_A 1.417 0.99
40_L 357_Q 1.402 0.99
377_A 396_W 1.401 0.99
195_R 401_E 1.399 0.99
287_R 322_K 1.395 0.99
394_R 399_I 1.394 0.99
301_K 372_E 1.375 0.98
93_N 260_Q 1.364 0.98
338_H 362_K 1.362 0.98
188_A 401_E 1.354 0.98
299_D 330_T 1.351 0.98
37_T 51_R 1.337 0.98
275_V 279_A 1.331 0.98
327_L 400_V 1.328 0.98
310_L 316_L 1.321 0.98
359_R 384_H 1.32 0.98
134_Q 137_L 1.317 0.98
259_G 276_S 1.315 0.98
324_L 375_V 1.314 0.98
306_C 320_V 1.314 0.98
190_A 194_D 1.294 0.98
243_A 280_V 1.287 0.97
280_V 288_E 1.285 0.97
211_A 215_H 1.282 0.97
359_R 363_M 1.273 0.97
69_Q 102_V 1.272 0.97
25_C 31_C 1.27 0.97
215_H 250_G 1.269 0.97
231_D 271_K 1.265 0.97
291_L 323_V 1.264 0.97
280_V 289_E 1.261 0.97
98_G 102_V 1.259 0.97
104_Q 220_E 1.255 0.97
237_I 278_L 1.248 0.97
252_G 314_Q 1.244 0.97
364_N 367_E 1.243 0.97
214_Y 254_F 1.232 0.96
32_T 47_G 1.23 0.96
38_Y 42_G 1.224 0.96
374_I 385_L 1.22 0.96
214_Y 219_Q 1.22 0.96
234_W 238_E 1.219 0.96
341_C 381_C 1.215 0.96
373_M 394_R 1.212 0.96
375_V 396_W 1.21 0.96
31_C 35_C 1.201 0.96
195_R 404_L 1.193 0.95
274_Q 277_E 1.19 0.95
77_T 309_T 1.182 0.95
192_V 397_I 1.177 0.95
286_L 323_V 1.171 0.95
165_R 199_S 1.171 0.95
360_D 367_E 1.171 0.95
169_R 201_M 1.169 0.95
34_T 84_T 1.156 0.94
32_T 44_E 1.155 0.94
25_C 35_C 1.154 0.94
286_L 400_V 1.144 0.94
44_E 50_G 1.144 0.94
225_R 228_N 1.139 0.94
137_L 141_P 1.138 0.94
136_G 139_L 1.137 0.94
367_E 389_G 1.136 0.94
170_V 245_L 1.129 0.93
171_L 241_A 1.125 0.93
135_V 139_L 1.123 0.93
322_K 326_R 1.117 0.93
176_C 248_A 1.115 0.93
323_V 400_V 1.115 0.93
214_Y 222_G 1.114 0.93
194_D 200_V 1.113 0.93
40_L 354_L 1.103 0.92
364_N 368_S 1.102 0.92
230_I 262_L 1.1 0.92
321_E 325_L 1.099 0.92
233_W 241_A 1.095 0.92
336_D 361_N 1.094 0.92
137_L 140_R 1.09 0.92
256_K 316_L 1.088 0.92
283_V 396_W 1.088 0.92
282_L 286_L 1.077 0.91
57_Q 62_N 1.074 0.91
203_A 206_A 1.073 0.91
46_D 74_R 1.07 0.91
284_E 319_E 1.068 0.91
283_V 377_A 1.064 0.90
47_G 51_R 1.061 0.90
82_E 92_H 1.059 0.90
246_Q 255_V 1.057 0.90
237_I 275_V 1.055 0.90
26_V 32_T 1.053 0.90
315_K 319_E 1.053 0.90
343_S 348_A 1.05 0.90
25_C 28_C 1.047 0.89
52_I 91_Y 1.046 0.89
300_K 373_M 1.046 0.89
325_L 330_T 1.041 0.89
268_Y 271_K 1.038 0.89
51_R 378_N 1.035 0.89
28_C 31_C 1.033 0.89
356_R 388_A 1.029 0.88
318_G 325_L 1.029 0.88
303_A 369_G 1.028 0.88
44_E 51_R 1.025 0.88
367_E 390_R 1.025 0.88
248_A 251_C 1.023 0.88
213_D 221_K 1.018 0.88
307_P 378_N 1.016 0.87
347_Y 351_H 1.016 0.87
288_E 322_K 1.009 0.87
169_R 243_A 1.006 0.87
318_G 321_E 1.005 0.87
280_V 285_L 1.005 0.87
36_P 89_V 1.003 0.87
17_E 20_S 1.003 0.87
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3kwlA10.85261000.451Contact Map0.516
3cf4A10.82561000.59Contact Map0.129
3c8yA10.793699.60.832Contact Map0.147
2wdqB30.262999.40.845Contact Map0.414
2h88B20.275299.40.846Contact Map0.414
1kf6B20.277699.40.848Contact Map0.408
3vr8B20.289999.40.848Contact Map0.428
2bs2B20.262999.10.866Contact Map0.422
1hfeL20.766698.10.905Contact Map0.128
3i9v920.358797.60.914Contact Map0.188
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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