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OPENSEQ.org
GSPK - Putative type II secretion system protein K
UniProt: P45762 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12894
Length: 327 (324)
Sequences: 461
Seq/Len: 1.42
GSPK
Paralog alert: 0.18 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
73_T 77_Q 3.193 1.00
153_D 164_E 3.168 1.00
100_A 103_C 2.85 1.00
127_F 194_I 2.755 1.00
155_D 164_E 2.665 1.00
103_C 207_C 2.577 1.00
150_D 153_D 2.542 1.00
167_F 171_Q 2.305 0.99
150_D 162_G 2.185 0.99
144_I 194_I 2.104 0.99
65_D 77_Q 2.026 0.98
153_D 162_G 2.007 0.98
65_D 73_T 1.941 0.97
155_D 162_G 1.919 0.97
153_D 179_N 1.908 0.97
150_D 155_D 1.831 0.96
150_D 164_E 1.801 0.95
151_Y 168_Y 1.701 0.93
130_L 228_L 1.638 0.91
175_R 190_Q 1.607 0.90
106_L 206_V 1.602 0.90
165_D 177_S 1.593 0.89
151_Y 175_R 1.592 0.89
134_A 206_V 1.584 0.89
150_D 179_N 1.58 0.89
97_L 291_L 1.577 0.89
100_A 207_C 1.519 0.86
141_T 195_T 1.506 0.85
182_L 191_I 1.505 0.85
155_D 179_N 1.498 0.85
256_T 259_A 1.482 0.84
218_L 260_F 1.457 0.83
307_H 319_I 1.454 0.83
284_P 289_F 1.45 0.82
75_L 80_A 1.444 0.82
105_N 210_P 1.444 0.82
124_A 145_V 1.444 0.82
150_D 163_A 1.44 0.82
164_E 168_Y 1.435 0.81
131_L 136_I 1.429 0.81
75_L 220_M 1.429 0.81
225_D 228_L 1.426 0.81
73_T 103_C 1.425 0.81
188_L 206_V 1.41 0.80
151_Y 187_E 1.408 0.80
5_Q 9_A 1.405 0.79
20_M 254_W 1.395 0.79
146_Q 161_H 1.389 0.78
164_E 179_N 1.388 0.78
77_Q 80_A 1.38 0.78
218_L 229_F 1.368 0.77
250_P 253_G 1.362 0.76
168_Y 175_R 1.345 0.75
5_Q 11_L 1.345 0.75
101_Q 209_L 1.336 0.74
257_T 284_P 1.335 0.74
93_V 103_C 1.332 0.74
47_R 51_I 1.32 0.73
55_E 322_R 1.307 0.72
103_C 253_G 1.294 0.71
103_C 224_N 1.274 0.69
153_D 168_Y 1.265 0.69
103_C 225_D 1.257 0.68
100_A 290_T 1.247 0.67
6_R 9_A 1.241 0.66
109_L 152_I 1.238 0.66
127_F 145_V 1.233 0.66
79_W 220_M 1.23 0.65
210_P 289_F 1.222 0.65
195_T 198_I 1.221 0.65
162_G 179_N 1.22 0.65
33_S 40_Q 1.216 0.64
317_A 322_R 1.209 0.63
143_E 147_S 1.201 0.63
5_Q 8_V 1.195 0.62
207_C 250_P 1.188 0.61
99_D 220_M 1.187 0.61
155_D 163_A 1.177 0.60
109_L 173_P 1.176 0.60
8_V 99_D 1.171 0.60
240_A 243_R 1.164 0.59
207_C 253_G 1.163 0.59
136_I 144_I 1.159 0.59
103_C 250_P 1.158 0.59
102_H 225_D 1.157 0.58
163_A 179_N 1.154 0.58
126_V 231_A 1.153 0.58
209_L 308_I 1.152 0.58
47_R 91_N 1.151 0.58
9_A 19_L 1.148 0.58
163_A 168_Y 1.146 0.57
153_D 163_A 1.142 0.57
99_D 286_S 1.14 0.57
11_L 14_L 1.132 0.56
185_T 206_V 1.13 0.56
77_Q 103_C 1.125 0.55
134_A 203_I 1.125 0.55
49_Y 254_W 1.124 0.55
77_Q 220_M 1.115 0.54
141_T 230_R 1.115 0.54
205_Y 225_D 1.111 0.54
7_G 10_L 1.11 0.54
107_N 151_Y 1.109 0.54
147_S 191_I 1.108 0.54
104_F 127_F 1.107 0.53
230_R 239_D 1.105 0.53
216_I 228_L 1.104 0.53
207_C 225_D 1.1 0.53
54_A 304_W 1.096 0.52
207_C 224_N 1.095 0.52
224_N 228_L 1.093 0.52
144_I 147_S 1.092 0.52
167_F 192_K 1.092 0.52
20_M 233_F 1.092 0.52
188_L 191_I 1.089 0.52
130_L 231_A 1.088 0.52
140_N 195_T 1.084 0.51
202_L 206_V 1.084 0.51
80_A 220_M 1.082 0.51
95_W 293_T 1.079 0.51
147_S 192_K 1.077 0.50
318_Q 323_T 1.068 0.49
216_I 257_T 1.064 0.49
194_I 198_I 1.063 0.49
33_S 39_R 1.062 0.49
38_T 86_Q 1.06 0.49
216_I 232_L 1.059 0.49
147_S 193_G 1.058 0.48
225_D 325_Q 1.056 0.48
106_L 248_K 1.053 0.48
237_I 245_L 1.052 0.48
62_L 65_D 1.051 0.48
128_S 201_R 1.05 0.48
107_N 187_E 1.05 0.48
50_D 87_L 1.044 0.47
53_L 247_Q 1.043 0.47
27_M 34_Q 1.043 0.47
100_A 308_I 1.042 0.47
26_D 192_K 1.041 0.47
167_F 170_S 1.039 0.47
250_P 254_W 1.036 0.46
175_R 187_E 1.034 0.46
215_S 283_F 1.033 0.46
319_I 323_T 1.033 0.46
226_I 230_R 1.031 0.46
76_Q 201_R 1.029 0.45
242_A 246_L 1.027 0.45
244_V 314_Q 1.026 0.45
244_V 280_R 1.025 0.45
150_D 168_Y 1.025 0.45
99_D 217_N 1.023 0.45
257_T 287_R 1.015 0.44
304_W 307_H 1.015 0.44
151_Y 182_L 1.013 0.44
62_L 188_L 1.008 0.43
228_L 231_A 1.007 0.43
106_L 245_L 1.004 0.43
23_L 297_S 1.003 0.43
106_L 256_T 1.002 0.43
173_P 201_R 1 0.43
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3ci0K10.8931000.268Contact Map0.507
2duyA10.229493.20.931Contact Map0.773
3arcU20.296692.60.932Contact Map0.477
2eduA10.2966880.939Contact Map0.451
1s5lU20.348686.20.94Contact Map0.639
2i5hA10.470978.50.945Contact Map0.31
3psiA10.84140.90.957Contact Map0.279
1wcnA10.20826.50.961Contact Map0.635
3psfA10.84421.80.963Contact Map0.305
2m7gA10.186519.10.964Contact Map0.203
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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