OPENSEQ.org - GSPH - ECOLI GREMLIN

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GSPH - Putative type II secretion system protein H

UniProt:	P41443	Sequence MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDVASTRARETAARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESQNDWDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLDQTLARDTRP	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG12887
Length:	169 (161)
Sequences:	332
Seq/Len:	2.06

GSPH
Paralog alert: 0.20 [within 20: 0.00] - ratio of genomes with paralogs

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
17_A	20_A	2.361	1.00
6_G	9_L	2.318	1.00
64_P	128_A	1.907	0.99
54_A	65_V	1.872	0.98
6_G	11_E	1.806	0.98
16_L	19_V	1.805	0.98
103_W	109_I	1.784	0.97
43_T	46_R	1.728	0.97
122_P	131_Q	1.69	0.96
7_F	11_E	1.646	0.95
12_M	23_A	1.614	0.95
66_G	123_Q	1.612	0.95
88_W	135_F	1.567	0.94
40_A	139_M	1.545	0.93
8_T	11_E	1.514	0.92
47_F	137_L	1.479	0.91
57_R	61_S	1.467	0.90
10_L	14_L	1.466	0.90
153_C	157_W	1.417	0.88
77_M	106_E	1.415	0.88
66_G	122_P	1.395	0.87
43_T	50_A	1.359	0.85
20_A	74_W	1.342	0.84
90_P	95_A	1.331	0.83
3_Q	99_S	1.308	0.82
136_S	152_V	1.3	0.81
8_T	15_V	1.3	0.81
31_G	38_T	1.29	0.81
24_S	28_F	1.287	0.81
58_A	128_A	1.287	0.81
51_L	126_I	1.274	0.80
47_F	151_L	1.273	0.80
40_A	113_P	1.26	0.79
15_V	137_L	1.254	0.78
57_R	94_D	1.251	0.78
66_G	131_Q	1.245	0.77
46_R	134_P	1.24	0.77
50_A	101_N	1.225	0.76
69_F	137_L	1.219	0.75
44_A	161_Q	1.214	0.75
26_V	50_A	1.176	0.72
60_L	88_W	1.162	0.70
74_W	129_D	1.161	0.70
94_D	98_E	1.158	0.70
113_P	117_D	1.142	0.68
1_M	146_E	1.142	0.68
51_L	94_D	1.141	0.68
135_F	153_C	1.137	0.68
122_P	160_D	1.132	0.67
66_G	135_F	1.12	0.66
94_D	97_D	1.12	0.66
13_M	113_P	1.118	0.66
50_A	74_W	1.115	0.66
109_I	141_N	1.11	0.65
39_R	59_T	1.106	0.65
22_T	25_V	1.09	0.63
111_L	137_L	1.085	0.62
29_T	63_Q	1.079	0.62
64_P	103_W	1.074	0.61
103_W	113_P	1.073	0.61
49_A	52_E	1.073	0.61
25_V	29_T	1.068	0.61
153_C	159_L	1.065	0.60
13_M	161_Q	1.062	0.60
13_M	57_R	1.06	0.60
18_L	54_A	1.059	0.60
60_L	74_W	1.058	0.60
122_P	135_F	1.053	0.59
89_V	124_V	1.053	0.59
26_V	158_P	1.044	0.58
20_A	144_T	1.043	0.58
12_M	122_P	1.041	0.58
66_G	88_W	1.04	0.58
126_I	132_I	1.036	0.57
55_I	126_I	1.033	0.57
49_A	125_V	1.028	0.56
113_P	130_G	1.028	0.56
10_L	141_N	1.025	0.56
56_D	74_W	1.02	0.55
64_P	129_D	1.02	0.55
131_Q	135_F	1.011	0.54
124_V	152_V	1.011	0.54
5_R	137_L	1.01	0.54
43_T	134_P	1.01	0.54
60_L	137_L	1.003	0.54
17_A	54_A	1.002	0.53
41_R	159_L	1	0.53

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
2knqA	1	0.8343	99.9	0.388	Contact Map	0.355
4dq9A	2	0.7692	99.9	0.432	Contact Map	0.428
1oqwA	2	0.787	98.3	0.778	Contact Map	0.277
3sokA	2	0.8225	97.8	0.801	Contact Map	0.334
2m7gA	1	0.3609	97.8	0.801	Contact Map	0.344
2hi2A	1	0.7988	97.4	0.819	Contact Map	0.335
2xr4A	2	0.426	13.5	0.935	Contact Map	0.257
2bv4A	2	0.4142	13.1	0.936	Contact Map	0.28
2chhA	1	0.4201	5.9	0.945	Contact Map	0.322
3fzyA	2	0.568	4.3	0.948	Contact Map	0.249

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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