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OPENSEQ.org
NAPA - Periplasmic nitrate reductase
UniProt: P33937 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12067
Length: 828 (811)
Sequences: 3858
Seq/Len: 4.76
NAPA
Paralog alert: 0.72 [within 20: 0.14] - ratio of genomes with paralogs
Cluster includes: BISC DMSA FDHF FDNG FDOG NAPA TORA TORZ YDEP YNFE YNFF
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
99_Q 117_P 6.73 1.00
758_L 777_V 6.58 1.00
125_D 568_R 5.997 1.00
765_K 826_E 5.293 1.00
270_Y 341_T 4.557 1.00
762_D 827_K 4.429 1.00
752_D 788_G 4.414 1.00
278_N 357_Q 4.314 1.00
714_L 756_R 4.242 1.00
124_F 568_R 4.204 1.00
274_Y 278_N 3.946 1.00
556_K 564_Q 3.907 1.00
339_E 349_K 3.858 1.00
748_I 777_V 3.718 1.00
547_Q 550_Q 3.605 1.00
768_S 823_V 3.527 1.00
765_K 772_E 3.418 1.00
560_W 564_Q 3.407 1.00
130_K 133_T 3.376 1.00
507_V 519_A 3.315 1.00
764_V 777_V 3.295 1.00
126_V 521_L 3.293 1.00
273_N 277_Q 3.211 1.00
342_A 349_K 3.156 1.00
134_A 142_S 3.142 1.00
118_I 521_L 3.095 1.00
272_A 329_Y 3.085 1.00
283_Q 287_S 3.066 1.00
272_A 393_L 3.056 1.00
267_I 345_T 3.041 1.00
127_M 521_L 3.018 1.00
102_L 521_L 3.009 1.00
758_L 764_V 2.976 1.00
748_I 825_L 2.93 1.00
133_T 137_E 2.91 1.00
117_P 554_E 2.838 1.00
751_L 787_Q 2.824 1.00
291_N 691_D 2.823 1.00
102_L 118_I 2.812 1.00
756_R 824_K 2.797 1.00
207_D 365_K 2.793 1.00
276_I 326_F 2.737 1.00
767_V 824_K 2.727 1.00
119_T 122_Q 2.72 1.00
753_A 779_T 2.717 1.00
212_W 268_L 2.704 1.00
767_V 826_E 2.673 1.00
743_E 776_I 2.632 1.00
99_Q 554_E 2.621 1.00
131_F 475_V 2.593 1.00
564_Q 568_R 2.531 1.00
124_F 564_Q 2.502 1.00
337_T 340_K 2.473 1.00
206_A 209_F 2.457 1.00
118_I 126_V 2.456 1.00
349_K 353_E 2.436 1.00
238_T 358_L 2.421 1.00
274_Y 338_L 2.418 1.00
273_N 338_L 2.397 1.00
263_S 344_M 2.382 1.00
102_L 126_V 2.344 1.00
348_P 351_Q 2.329 1.00
274_Y 357_Q 2.308 1.00
746_L 790_V 2.306 1.00
241_V 253_A 2.305 1.00
127_M 506_I 2.3 1.00
760_R 779_T 2.3 1.00
281_I 286_F 2.27 1.00
281_I 326_F 2.266 1.00
489_N 807_D 2.257 1.00
342_A 348_P 2.248 1.00
767_V 772_E 2.244 1.00
255_N 354_Q 2.237 1.00
351_Q 354_Q 2.193 1.00
339_E 353_E 2.161 1.00
691_D 695_V 2.147 1.00
102_L 116_T 2.142 1.00
274_Y 280_A 2.14 1.00
746_L 764_V 2.135 1.00
104_M 116_T 2.118 1.00
279_N 283_Q 2.11 1.00
354_Q 358_L 2.092 1.00
126_V 130_K 2.092 1.00
54_G 73_A 2.069 1.00
56_L 71_P 2.064 1.00
278_N 353_E 2.064 1.00
775_S 792_M 2.063 1.00
257_I 347_V 2.059 1.00
276_I 333_V 2.032 1.00
257_I 355_L 2.024 1.00
208_A 240_A 2.022 1.00
98_T 553_G 2.022 1.00
399_Q 402_C 2.013 1.00
101_L 522_I 2.01 1.00
512_P 516_A 2.009 1.00
280_A 360_A 1.99 1.00
504_F 521_L 1.988 1.00
284_D 288_K 1.977 1.00
393_L 696_I 1.976 1.00
122_Q 126_V 1.967 1.00
341_T 352_L 1.957 1.00
266_V 344_M 1.953 1.00
790_V 823_V 1.948 1.00
477_W 508_S 1.945 1.00
552_P 555_A 1.943 1.00
755_A 787_Q 1.938 1.00
280_A 357_Q 1.923 1.00
271_I 359_Y 1.922 1.00
208_A 358_L 1.916 1.00
474_N 503_N 1.909 1.00
210_V 242_L 1.905 1.00
763_K 774_I 1.899 1.00
719_G 791_Y 1.897 1.00
706_E 770_R 1.892 1.00
290_V 696_I 1.88 1.00
267_I 352_L 1.874 1.00
134_A 475_V 1.867 1.00
122_Q 521_L 1.85 1.00
359_Y 394_T 1.85 1.00
276_I 330_K 1.837 1.00
746_L 792_M 1.824 1.00
275_I 281_I 1.816 1.00
709_D 769_R 1.796 1.00
765_K 774_I 1.795 1.00
255_N 358_L 1.774 1.00
205_Q 400_P 1.766 1.00
267_I 355_L 1.764 1.00
746_L 766_V 1.756 1.00
507_V 516_A 1.75 1.00
756_R 825_L 1.733 1.00
259_F 347_V 1.727 1.00
328_D 331_A 1.726 1.00
255_N 351_Q 1.719 1.00
747_F 780_R 1.711 0.99
342_A 352_L 1.71 0.99
763_K 776_I 1.705 0.99
138_K 474_N 1.705 0.99
336_Y 341_T 1.7 0.99
244_T 256_G 1.698 0.99
277_Q 353_E 1.696 0.99
714_L 790_V 1.684 0.99
714_L 824_K 1.68 0.99
126_V 504_F 1.671 0.99
331_A 334_A 1.661 0.99
532_E 547_Q 1.659 0.99
231_R 254_D 1.658 0.99
131_F 506_I 1.656 0.99
760_R 778_E 1.655 0.99
773_V 792_M 1.652 0.99
497_G 500_D 1.651 0.99
230_R 237_V 1.649 0.99
100_P 120_W 1.647 0.99
748_I 790_V 1.641 0.99
101_L 117_P 1.634 0.99
286_F 326_F 1.63 0.99
241_V 250_F 1.628 0.99
209_F 227_I 1.617 0.99
759_R 762_D 1.615 0.99
208_A 355_L 1.614 0.99
340_K 343_E 1.612 0.99
502_R 505_I 1.61 0.99
780_R 784_R 1.606 0.99
270_Y 352_L 1.6 0.99
748_I 758_L 1.595 0.99
242_L 267_I 1.583 0.99
274_Y 353_E 1.576 0.99
556_K 560_W 1.576 0.99
714_L 752_D 1.568 0.99
263_S 345_T 1.567 0.99
359_Y 368_S 1.567 0.99
118_I 122_Q 1.563 0.99
359_Y 366_V 1.56 0.99
127_M 523_L 1.544 0.99
49_C 53_C 1.536 0.99
564_Q 567_R 1.529 0.99
766_V 792_M 1.5 0.98
271_I 275_I 1.5 0.98
478_T 505_I 1.495 0.98
124_F 560_W 1.494 0.98
42_D 59_T 1.492 0.98
134_A 503_N 1.492 0.98
207_D 362_P 1.49 0.98
163_F 169_S 1.484 0.98
89_K 736_E 1.479 0.98
751_L 754_K 1.473 0.98
57_V 64_V 1.469 0.98
98_T 117_P 1.465 0.98
508_S 561_Q 1.459 0.98
780_R 785_P 1.457 0.98
276_I 281_I 1.457 0.98
746_L 825_L 1.445 0.98
132_K 136_K 1.438 0.98
526_A 557_S 1.436 0.98
106_N 112_E 1.43 0.98
97_L 522_I 1.426 0.97
64_V 91_M 1.424 0.97
255_N 355_L 1.42 0.97
496_P 500_D 1.417 0.97
202_D 205_Q 1.409 0.97
58_G 68_Q 1.397 0.97
342_A 347_V 1.393 0.97
342_A 345_T 1.393 0.97
139_G 142_S 1.389 0.97
286_F 696_I 1.387 0.97
240_A 355_L 1.386 0.97
284_D 287_S 1.386 0.97
396_K 402_C 1.382 0.97
209_F 237_V 1.379 0.97
210_V 271_I 1.376 0.97
46_C 49_C 1.373 0.97
273_N 333_V 1.37 0.97
792_M 801_V 1.368 0.97
277_Q 334_A 1.364 0.96
336_Y 344_M 1.354 0.96
560_W 567_R 1.354 0.96
129_E 133_T 1.351 0.96
336_Y 340_K 1.349 0.96
273_N 337_T 1.346 0.96
512_P 522_I 1.343 0.96
770_R 805_T 1.342 0.96
526_A 555_A 1.339 0.96
372_M 376_Q 1.339 0.96
268_L 387_V 1.337 0.96
714_L 825_L 1.332 0.96
689_K 695_V 1.323 0.96
125_D 129_E 1.323 0.96
124_F 556_K 1.319 0.95
131_F 569_F 1.315 0.95
67_C 88_P 1.314 0.95
210_V 355_L 1.311 0.95
246_Q 251_E 1.31 0.95
104_M 114_E 1.304 0.95
44_A 57_V 1.302 0.95
466_R 494_R 1.3 0.95
362_P 365_K 1.295 0.95
711_E 769_R 1.295 0.95
69_G 78_G 1.293 0.95
366_V 396_K 1.292 0.95
116_T 122_Q 1.291 0.95
690_P 695_V 1.29 0.95
728_G 732_R 1.283 0.95
115_F 522_I 1.283 0.95
261_P 789_L 1.278 0.94
132_K 135_L 1.273 0.94
549_V 552_P 1.268 0.94
144_G 473_L 1.267 0.94
749_H 787_Q 1.266 0.94
610_A 631_E 1.261 0.94
346_G 786_P 1.258 0.94
326_F 330_K 1.254 0.94
270_Y 274_Y 1.254 0.94
274_Y 352_L 1.25 0.93
335_E 340_K 1.245 0.93
277_Q 338_L 1.243 0.93
57_V 529_V 1.24 0.93
209_F 230_R 1.239 0.93
714_L 749_H 1.239 0.93
362_P 366_V 1.238 0.93
317_G 328_D 1.237 0.93
242_L 355_L 1.237 0.93
440_D 444_K 1.234 0.93
211_L 214_A 1.231 0.93
758_L 779_T 1.222 0.92
257_I 351_Q 1.221 0.92
275_I 360_A 1.22 0.92
750_P 783_N 1.219 0.92
338_L 353_E 1.214 0.92
144_G 464_Q 1.213 0.92
341_T 345_T 1.203 0.92
135_L 143_I 1.202 0.92
282_N 399_Q 1.201 0.92
469_K 494_R 1.196 0.91
286_F 393_L 1.196 0.91
271_I 356_A 1.195 0.91
134_A 504_F 1.195 0.91
329_Y 393_L 1.195 0.91
274_Y 349_K 1.191 0.91
631_E 634_A 1.188 0.91
43_K 56_L 1.186 0.91
273_N 334_A 1.183 0.91
162_L 569_F 1.183 0.91
54_G 70_D 1.179 0.91
120_W 523_L 1.176 0.90
57_V 67_C 1.173 0.90
475_V 506_I 1.171 0.90
747_F 778_E 1.17 0.90
745_V 778_E 1.17 0.90
231_R 252_L 1.168 0.90
211_L 239_V 1.165 0.90
272_A 333_V 1.161 0.90
748_I 779_T 1.16 0.90
374_F 387_V 1.16 0.90
527_M 552_P 1.159 0.90
46_C 53_C 1.156 0.89
274_Y 277_Q 1.153 0.89
95_D 733_R 1.15 0.89
198_G 202_D 1.147 0.89
523_L 561_Q 1.145 0.89
661_V 665_E 1.145 0.89
186_G 384_N 1.145 0.89
479_M 562_L 1.143 0.89
100_P 119_T 1.14 0.89
185_V 812_L 1.137 0.88
202_D 401_G 1.135 0.88
510_P 527_M 1.134 0.88
464_Q 473_L 1.134 0.88
143_I 475_V 1.133 0.88
63_R 92_Y 1.133 0.88
56_L 70_D 1.13 0.88
121_D 568_R 1.129 0.88
431_M 441_I 1.126 0.88
281_I 393_L 1.126 0.88
532_E 544_F 1.123 0.88
127_M 504_F 1.123 0.88
350_D 353_E 1.111 0.87
259_F 263_S 1.11 0.87
130_K 134_A 1.109 0.87
329_Y 333_V 1.107 0.87
5_R 8_F 1.107 0.87
337_T 341_T 1.104 0.86
251_E 784_R 1.1 0.86
120_W 561_Q 1.098 0.86
482_N 727_T 1.097 0.86
746_L 775_S 1.096 0.86
775_S 801_V 1.093 0.86
337_T 342_A 1.09 0.85
790_V 825_L 1.087 0.85
204_E 226_R 1.086 0.85
532_E 546_R 1.084 0.85
342_A 350_D 1.084 0.85
469_K 497_G 1.082 0.85
766_V 775_S 1.078 0.85
45_P 543_Q 1.073 0.84
357_Q 361_D 1.073 0.84
142_S 474_N 1.065 0.84
182_S 815_E 1.063 0.83
98_T 552_P 1.063 0.83
260_T 786_P 1.062 0.83
268_L 386_L 1.062 0.83
240_A 255_N 1.06 0.83
338_L 349_K 1.059 0.83
217_A 249_S 1.059 0.83
349_K 352_L 1.058 0.83
477_W 565_F 1.057 0.83
770_R 822_A 1.057 0.83
131_F 477_W 1.056 0.83
274_Y 356_A 1.055 0.83
280_A 361_D 1.055 0.83
103_R 115_F 1.05 0.82
508_S 525_T 1.047 0.82
326_F 393_L 1.046 0.82
275_I 390_L 1.045 0.82
353_E 357_Q 1.044 0.82
392_L 694_A 1.044 0.82
212_W 242_L 1.043 0.82
476_Y 502_R 1.041 0.82
59_T 65_V 1.039 0.81
185_V 381_V 1.038 0.81
124_F 565_F 1.038 0.81
557_S 560_W 1.038 0.81
270_Y 337_T 1.038 0.81
716_L 823_V 1.037 0.81
475_V 504_F 1.035 0.81
155_E 531_K 1.033 0.81
469_K 501_P 1.031 0.81
92_Y 552_P 1.027 0.80
329_Y 696_I 1.024 0.80
246_Q 783_N 1.023 0.80
62_G 550_Q 1.017 0.79
770_R 821_C 1.016 0.79
62_G 65_V 1.012 0.79
274_Y 360_A 1.008 0.79
238_T 254_D 1.007 0.79
105_K 112_E 1.005 0.78
44_A 545_W 1.004 0.78
149_G 174_P 1.001 0.78
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3ml1A10.96381000.188Contact Map0.749
1kqfA10.97341000.222Contact Map0.75
1h0hA20.94081000.258Contact Map0.74
2napA10.85871000.266Contact Map0.783
2ivfA10.94571000.266Contact Map0.751
2iv2X10.82251000.266Contact Map0.78
2vpzA20.84541000.279Contact Map0.744
1q16A10.98311000.29Contact Map0.7
2e7zA10.80311000.299Contact Map0.757
1tmoA10.85511000.323Contact Map0.693
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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