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OPENSEQ.org
LLDD - L-lactate dehydrogenase [cytochrome]
UniProt: P33232 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11963
Length: 396 (376)
Sequences: 1791
Seq/Len: 4.76
LLDD
Paralog alert: 0.31 [within 20: 0.02] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
341_Q 345_A 4.199 1.00
145_K 245_D 3.448 1.00
105_L 116_V 3.38 1.00
114_E 143_R 3.304 1.00
335_A 347_L 3.213 1.00
366_S 369_E 3.157 1.00
316_V 358_A 2.964 1.00
58_L 356_K 2.902 1.00
61_E 68_K 2.888 1.00
94_K 341_Q 2.846 1.00
63_T 68_K 2.753 1.00
304_L 327_L 2.736 1.00
145_K 244_W 2.488 1.00
165_Y 311_N 2.465 1.00
32_E 166_R 2.459 1.00
78_V 333_L 2.444 1.00
241_R 268_D 2.431 1.00
98_A 341_Q 2.424 1.00
316_V 355_M 2.371 1.00
73_V 315_V 2.365 1.00
365_K 369_E 2.315 1.00
47_L 362_T 2.281 1.00
261_D 265_F 2.274 1.00
263_V 301_I 2.247 1.00
134_R 236_D 2.231 1.00
315_V 351_I 2.194 1.00
74_A 325_T 2.174 1.00
235_K 238_E 2.107 1.00
302_A 324_D 2.104 1.00
256_P 292_A 2.103 1.00
42_L 305_A 2.094 1.00
295_D 372_Q 2.084 1.00
41_D 285_S 2.063 1.00
259_A 270_I 2.032 1.00
134_R 239_W 2.004 1.00
104_T 153_V 1.987 1.00
316_V 370_I 1.98 1.00
259_A 297_V 1.975 1.00
98_A 345_A 1.951 1.00
138_R 142_E 1.936 1.00
138_R 243_F 1.932 1.00
6_A 347_L 1.917 1.00
261_D 264_R 1.906 1.00
74_A 327_L 1.893 1.00
154_F 237_L 1.879 1.00
75_L 96_A 1.879 1.00
28_G 35_L 1.814 1.00
141_L 243_F 1.814 1.00
71_M 352_E 1.793 1.00
250_I 262_A 1.779 1.00
10_R 26_D 1.743 1.00
352_E 356_K 1.72 1.00
99_H 349_N 1.718 1.00
45_V 287_A 1.682 0.99
25_M 84_Y 1.675 0.99
260_R 296_A 1.627 0.99
42_L 287_A 1.623 0.99
71_M 348_L 1.621 0.99
11_A 14_Q 1.597 0.99
48_R 292_A 1.582 0.99
294_A 303_I 1.564 0.99
156_V 250_I 1.561 0.99
48_R 255_D 1.557 0.99
142_E 243_F 1.551 0.99
141_L 152_L 1.548 0.99
70_S 100_G 1.512 0.98
52_L 156_V 1.501 0.98
260_R 300_D 1.498 0.98
249_V 269_G 1.497 0.98
289_A 292_A 1.496 0.98
271_V 327_L 1.493 0.98
319_I 367_I 1.487 0.98
81_C 92_A 1.477 0.98
38_N 274_N 1.471 0.98
67_E 150_S 1.468 0.98
151_T 249_V 1.458 0.98
36_R 40_E 1.444 0.98
272_V 289_A 1.441 0.98
285_S 288_R 1.439 0.98
65_F 269_G 1.436 0.98
99_H 345_A 1.422 0.97
305_A 309_I 1.407 0.97
92_A 103_F 1.406 0.97
113_I 149_C 1.399 0.97
105_L 149_C 1.394 0.97
19_P 23_H 1.393 0.97
237_L 267_A 1.391 0.97
54_N 233_S 1.388 0.97
364_A 370_I 1.379 0.97
75_L 92_A 1.371 0.97
142_E 146_A 1.37 0.97
130_V 232_I 1.367 0.97
50_R 255_D 1.361 0.96
135_G 138_R 1.357 0.96
138_R 239_W 1.353 0.96
9_Y 346_N 1.351 0.96
95_A 341_Q 1.348 0.96
239_W 243_F 1.345 0.96
346_N 349_N 1.345 0.96
58_L 360_T 1.345 0.96
145_K 246_G 1.333 0.96
73_V 351_I 1.332 0.96
23_H 170_S 1.329 0.96
9_Y 347_L 1.329 0.96
364_A 374_S 1.327 0.96
142_E 145_K 1.323 0.96
303_I 324_D 1.317 0.95
328_L 351_I 1.31 0.95
292_A 376_V 1.306 0.95
64_L 325_T 1.299 0.95
342_A 345_A 1.292 0.95
60_L 355_M 1.278 0.94
241_R 248_M 1.278 0.94
31_S 311_N 1.277 0.94
22_F 26_D 1.275 0.94
364_A 369_E 1.272 0.94
151_T 247_P 1.266 0.94
269_G 302_A 1.263 0.94
310_R 331_A 1.261 0.94
305_A 318_M 1.261 0.94
75_L 348_L 1.26 0.94
356_K 360_T 1.253 0.94
260_R 264_R 1.25 0.93
284_L 289_A 1.247 0.93
167_D 170_S 1.24 0.93
294_A 299_G 1.238 0.93
95_A 348_L 1.236 0.93
304_L 325_T 1.236 0.93
75_L 332_F 1.233 0.93
259_A 293_I 1.231 0.93
14_Q 22_F 1.228 0.93
315_V 355_M 1.222 0.92
44_E 288_R 1.217 0.92
270_I 293_I 1.213 0.92
54_N 235_K 1.212 0.92
294_A 324_D 1.202 0.92
141_L 240_I 1.199 0.92
134_R 229_D 1.195 0.91
250_I 267_A 1.194 0.91
32_E 36_R 1.193 0.91
153_V 271_V 1.191 0.91
282_G 317_R 1.19 0.91
65_F 249_V 1.177 0.90
345_A 349_N 1.171 0.90
51_I 253_I 1.164 0.90
47_L 316_V 1.158 0.90
62_T 367_I 1.153 0.89
164_R 167_D 1.145 0.89
11_A 15_R 1.144 0.89
76_A 327_L 1.141 0.89
293_I 296_A 1.139 0.88
182_Y 186_V 1.138 0.88
25_M 79_G 1.129 0.88
90_V 111_C 1.125 0.88
73_V 348_L 1.123 0.88
59_S 68_K 1.123 0.88
253_I 259_A 1.12 0.87
41_D 288_R 1.118 0.87
77_P 104_T 1.117 0.87
37_R 46_A 1.113 0.87
92_A 332_F 1.112 0.87
239_W 242_D 1.101 0.86
63_T 302_A 1.099 0.86
97_D 121_K 1.099 0.86
65_F 302_A 1.099 0.86
287_A 305_A 1.097 0.86
272_V 286_S 1.091 0.85
8_D 169_H 1.085 0.85
238_E 242_D 1.08 0.85
5_A 165_Y 1.077 0.84
64_L 304_L 1.076 0.84
254_L 283_V 1.071 0.84
87_R 91_Q 1.07 0.84
34_T 282_G 1.068 0.84
60_L 71_M 1.068 0.84
238_E 265_F 1.067 0.84
359_M 367_I 1.06 0.83
37_R 44_E 1.06 0.83
309_I 326_V 1.06 0.83
71_M 101_I 1.057 0.83
85_A 91_Q 1.057 0.83
125_W 153_V 1.056 0.83
69_L 302_A 1.055 0.83
36_R 43_S 1.055 0.83
371_T 374_S 1.054 0.83
291_P 295_D 1.052 0.82
143_R 147_A 1.048 0.82
286_S 305_A 1.046 0.82
259_A 303_I 1.04 0.81
162_G 361_L 1.04 0.81
316_V 374_S 1.04 0.81
10_R 22_F 1.039 0.81
233_S 236_D 1.039 0.81
39_V 43_S 1.038 0.81
190_Q 194_D 1.034 0.81
138_R 242_D 1.03 0.81
263_V 300_D 1.026 0.80
254_L 289_A 1.023 0.80
124_M 149_C 1.02 0.80
48_R 257_E 1.018 0.80
51_I 156_V 1.018 0.80
114_E 147_A 1.015 0.79
48_R 256_P 1.014 0.79
79_G 108_V 1.008 0.79
107_T 128_L 1.005 0.78
313_L 358_A 1 0.78
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2nzlA10.91411000.389Contact Map0.765
1kbiA20.92931000.414Contact Map0.775
1goxA10.91921000.422Contact Map0.754
3sgzA30.88381000.423Contact Map0.763
2nliA20.89391000.43Contact Map0.746
1p4cA10.92681000.442Contact Map0.787
2qr6A10.81061000.619Contact Map0.474
1vcfA20.74491000.627Contact Map0.541
1p0kA20.78791000.656Contact Map0.612
3vkjA40.82581000.657Contact Map0.686
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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