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OPENSEQ.org
NRFG - Formate-dependent nitrite reductase complex subunit NrfG
UniProt: P32712 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11950
Length: 198 (186)
Sequences: 524
Seq/Len: 2.82
NRFG
Paralog alert: 0.49 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
159_N 162_Q 3.112 1.00
88_D 91_N 2.867 1.00
97_R 113_L 2.788 1.00
114_A 133_I 2.769 1.00
98_Q 101_Q 2.736 1.00
143_E 146_A 2.498 1.00
180_R 184_V 2.336 1.00
171_M 180_R 1.99 1.00
63_Q 83_Y 1.85 0.99
112_A 145_T 1.833 0.99
135_K 138_A 1.794 0.99
26_Y 30_P 1.771 0.99
113_L 117_L 1.645 0.98
187_I 190_A 1.622 0.97
73_S 103_R 1.616 0.97
167_W 190_A 1.613 0.97
108_E 143_E 1.608 0.97
107_A 136_A 1.603 0.97
151_A 167_W 1.537 0.96
69_N 72_N 1.527 0.96
107_A 110_Y 1.494 0.95
155_F 160_Y 1.489 0.95
66_I 75_Q 1.477 0.95
75_Q 95_A 1.471 0.94
62_L 81_E 1.464 0.94
174_N 177_R 1.444 0.94
83_Y 88_D 1.421 0.93
81_E 85_W 1.377 0.91
93_L 116_V 1.358 0.91
115_T 145_T 1.351 0.90
35_V 38_E 1.336 0.90
111_A 143_E 1.325 0.89
111_A 146_A 1.323 0.89
76_W 80_G 1.319 0.89
114_A 118_Y 1.313 0.89
78_L 113_L 1.287 0.87
186_S 190_A 1.28 0.87
135_K 139_L 1.275 0.87
174_N 183_L 1.266 0.86
110_Y 132_M 1.254 0.85
164_I 190_A 1.249 0.85
110_Y 135_K 1.249 0.85
103_R 106_N 1.247 0.85
147_L 173_L 1.235 0.84
100_L 110_Y 1.226 0.84
148_M 152_S 1.222 0.83
83_Y 91_N 1.222 0.83
152_S 186_S 1.221 0.83
16_I 21_L 1.19 0.81
171_M 184_V 1.187 0.81
63_Q 88_D 1.181 0.80
114_A 149_L 1.177 0.80
97_R 128_Q 1.174 0.80
80_G 96_Y 1.17 0.79
17_L 24_G 1.169 0.79
148_M 186_S 1.133 0.76
60_Q 63_Q 1.131 0.76
142_N 173_L 1.126 0.76
98_Q 104_G 1.124 0.76
20_F 23_V 1.12 0.75
66_I 76_W 1.117 0.75
19_V 22_C 1.112 0.74
76_W 95_A 1.103 0.74
155_F 190_A 1.087 0.72
160_Y 190_A 1.083 0.72
76_W 99_A 1.081 0.71
115_T 152_S 1.081 0.71
59_L 63_Q 1.071 0.70
36_R 80_G 1.065 0.70
24_G 183_L 1.061 0.69
184_V 188_N 1.058 0.69
137_L 146_A 1.057 0.69
37_A 135_K 1.048 0.68
163_A 190_A 1.041 0.67
9_K 12_T 1.035 0.67
31_K 34_A 1.034 0.67
54_T 57_A 1.031 0.66
105_E 135_K 1.031 0.66
65_K 72_N 1.027 0.66
129_T 132_M 1.025 0.66
78_L 81_E 1.02 0.65
136_A 146_A 1.019 0.65
147_L 160_Y 1.019 0.65
14_L 21_L 1.018 0.65
155_F 164_I 1.008 0.64
11_L 21_L 1.008 0.64
17_L 154_A 1.005 0.63
154_A 159_N 1.002 0.63
66_I 96_Y 1 0.63
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2e2eA20.853599.80.577Contact Map0.678
2vsyA20.732399.50.684Contact Map0.686
2pziA20.954599.40.691Contact Map0.614
4i2zA10.636499.40.697Contact Map0.709
4gywA20.813199.40.699Contact Map0.768
2c2lA40.636499.40.7Contact Map0.704
3upvA10.621299.40.704Contact Map0.761
3urzA20.787999.40.705Contact Map0.551
1dceA20.858699.40.705Contact Map0.427
2xcbA20.707199.30.714Contact Map0.663
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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