OPENSEQ.org - PTFA - ECOLI GREMLIN

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PTFA - Fructose-like phosphotransferase enzyme IIA component

UniProt:	P32155	Sequence MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQTLLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGVPQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG11864
Length:	148 (143)
Sequences:	3496
Seq/Len:	24.45

PTFA
Paralog alert: 0.74 [within 20: 0.21] - ratio of genomes with paralogs
Cluster includes: PTFA PTKA PTMA PTSN SGCA ULAC

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
95_N	131_Q	3.548	1.00
6_A	137_Q	2.825	1.00
27_T	31_Q	2.385	1.00
36_T	71_K	2.377	1.00
17_G	20_S	2.29	1.00
135_T	138_V	2.282	1.00
23_K	42_L	2.176	1.00
128_H	132_Q	2.123	1.00
25_L	99_C	2.082	1.00
14_Q	81_A	2.049	1.00
12_N	79_R	2.029	1.00
45_L	99_C	1.997	1.00
80_K	97_W	1.973	1.00
26_A	42_L	1.93	1.00
15_G	21_V	1.918	1.00
10_D	28_I	1.897	1.00
74_F	101_G	1.852	1.00
30_L	35_I	1.841	1.00
43_Q	47_L	1.791	1.00
12_N	81_A	1.788	1.00
34_F	72_Q	1.775	1.00
15_G	24_Q	1.755	1.00
83_A	95_N	1.714	1.00
21_V	97_W	1.658	1.00
25_L	78_A	1.648	1.00
19_Y	50_K	1.638	1.00
134_D	138_V	1.61	1.00
40_Q	69_C	1.58	1.00
36_T	40_Q	1.558	1.00
80_K	83_A	1.539	1.00
13_I	78_A	1.525	1.00
16_N	20_S	1.524	1.00
70_V	101_G	1.491	1.00
22_L	45_L	1.482	1.00
41_F	101_G	1.477	1.00
8_C	32_N	1.448	1.00
10_D	24_Q	1.431	1.00
7_S	32_N	1.42	1.00
47_L	50_K	1.409	1.00
49_E	54_T	1.406	0.99
27_T	38_S	1.392	0.99
137_Q	140_A	1.362	0.99
34_F	74_F	1.339	0.99
127_I	131_Q	1.311	0.99
26_A	45_L	1.305	0.99
68_A	104_Q	1.305	0.99
94_V	97_W	1.28	0.99
28_I	31_Q	1.278	0.99
133_G	138_V	1.236	0.98
80_K	95_N	1.236	0.98
11_L	79_R	1.224	0.98
106_G	109_D	1.218	0.98
19_Y	23_K	1.212	0.98
22_L	42_L	1.207	0.98
19_Y	46_L	1.204	0.98
15_G	80_K	1.204	0.98
48_R	54_T	1.166	0.97
26_A	99_C	1.164	0.97
37_D	40_Q	1.156	0.97
133_G	139_L	1.146	0.97
23_K	46_L	1.115	0.96
58_S	127_I	1.108	0.96
73_P	103_P	1.106	0.96
110_Q	114_I	1.104	0.96
133_G	141_L	1.094	0.96
20_S	23_K	1.082	0.96
59_G	95_N	1.081	0.95
115_G	118_C	1.072	0.95
9_I	139_L	1.07	0.95
29_A	101_G	1.067	0.95
47_L	51_M	1.055	0.95
30_L	38_S	1.053	0.95
78_A	99_C	1.043	0.94
13_I	24_Q	1.038	0.94
6_A	136_D	1.038	0.94
77_F	96_C	1.037	0.94
85_D	93_D	1.02	0.93
79_R	96_C	1.018	0.93
28_I	32_N	1.016	0.93
73_P	102_V	1.012	0.93
55_G	89_S	1.01	0.93

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
1a6jA	2	0.9865	100	0.059	Contact Map	0.743
2a0jA	1	0.9662	100	0.087	Contact Map	0.685
3urrA	2	0.9797	100	0.091	Contact Map	0.755
1a3aA	4	0.9527	100	0.095	Contact Map	0.802
3lf6A	2	0.973	100	0.102	Contact Map	0.737
3oxpA	2	0.9527	100	0.105	Contact Map	0.763
2oq3A	1	0.9459	100	0.109	Contact Map	0.677
3bjvA	1	0.9595	100	0.119	Contact Map	0.811
2oqtA	4	0.9595	100	0.123	Contact Map	0.823
3t43A	2	0.8716	100	0.163	Contact Map	0.776

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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