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OPENSEQ.org
THIH - 2-iminoacetate synthase
UniProt: P30140 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11590
Length: 377 (342)
Sequences: 2616
Seq/Len: 7.65
THIH
Paralog alert: 0.60 [within 20: 0.11] - ratio of genomes with paralogs
Cluster includes: BIOB THIH
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
142_R 169_E 4.738 1.00
164_E 214_R 4.264 1.00
187_E 203_F 3.851 1.00
64_L 289_L 3.825 1.00
107_E 143_R 3.17 1.00
149_R 176_D 3.158 1.00
106_D 109_D 3.067 1.00
62_Q 66_R 3.042 1.00
188_A 236_S 2.691 1.00
138_M 142_R 2.687 1.00
110_I 144_H 2.632 1.00
164_E 211_R 2.623 1.00
115_A 151_Q 2.523 1.00
203_F 207_E 2.501 1.00
139_D 169_E 2.462 1.00
164_E 168_A 2.441 1.00
107_E 147_A 2.336 1.00
111_A 147_A 2.261 1.00
88_D 97_S 2.22 1.00
257_V 291_A 2.208 1.00
112_R 115_A 2.204 1.00
37_L 289_L 2.191 1.00
210_D 252_Q 2.063 1.00
244_L 290_L 2.027 1.00
143_R 146_P 2.006 1.00
295_E 316_N 1.999 1.00
117_I 122_F 1.996 1.00
30_R 41_D 1.968 1.00
45_L 286_A 1.965 1.00
186_H 236_S 1.948 1.00
168_A 172_Q 1.928 1.00
147_A 150_E 1.903 1.00
46_L 286_A 1.869 1.00
60_R 289_L 1.864 1.00
296_L 315_I 1.834 1.00
177_G 219_K 1.799 1.00
115_A 119_E 1.77 1.00
65_T 359_L 1.765 1.00
346_R 349_A 1.753 1.00
142_R 146_P 1.738 1.00
114_S 148_L 1.732 1.00
109_D 112_R 1.705 1.00
72_T 360_Q 1.693 1.00
39_R 241_E 1.688 1.00
29_E 56_Q 1.687 1.00
171_K 217_I 1.687 1.00
233_R 236_S 1.652 1.00
66_R 71_N 1.645 1.00
188_A 238_M 1.638 1.00
210_D 249_H 1.636 1.00
351_A 355_T 1.63 1.00
165_T 168_A 1.625 1.00
42_M 290_L 1.572 1.00
43_M 47_S 1.563 1.00
207_E 211_R 1.551 1.00
169_E 172_Q 1.545 1.00
111_A 152_F 1.536 1.00
168_A 171_K 1.53 1.00
39_R 238_M 1.527 1.00
112_R 116_A 1.523 1.00
171_K 215_A 1.521 1.00
298_L 310_V 1.485 0.99
352_A 356_A 1.483 0.99
63_R 67_Q 1.482 0.99
188_A 192_R 1.477 0.99
111_A 115_A 1.477 0.99
38_T 41_D 1.467 0.99
43_M 235_D 1.465 0.99
355_T 361_P 1.458 0.99
62_Q 312_P 1.437 0.99
244_L 248_Q 1.432 0.99
39_R 244_L 1.427 0.99
62_Q 359_L 1.399 0.99
116_A 119_E 1.377 0.99
146_P 173_L 1.373 0.99
58_A 313_L 1.365 0.99
163_A 166_E 1.358 0.99
182_Q 239_V 1.352 0.99
37_L 42_M 1.341 0.99
167_Y 215_A 1.335 0.98
104_T 132_H 1.333 0.98
201_D 204_W 1.332 0.98
90_T 193_H 1.325 0.98
182_Q 202_F 1.324 0.98
204_W 207_E 1.311 0.98
180_V 205_R 1.31 0.98
162_L 170_L 1.308 0.98
62_Q 65_T 1.305 0.98
222_L 246_L 1.304 0.98
281_V 310_V 1.296 0.98
146_P 150_E 1.27 0.98
54_L 58_A 1.27 0.98
53_Y 56_Q 1.27 0.98
159_V 300_T 1.266 0.98
141_F 159_V 1.256 0.97
185_Y 235_D 1.253 0.97
29_E 33_N 1.249 0.97
146_P 172_Q 1.236 0.97
71_N 359_L 1.233 0.97
312_P 357_Q 1.228 0.97
147_A 151_Q 1.225 0.97
138_M 157_M 1.22 0.97
158_E 299_S 1.217 0.97
240_A 290_L 1.214 0.97
46_L 283_T 1.214 0.97
210_D 214_R 1.213 0.97
188_A 235_D 1.21 0.97
39_R 43_M 1.21 0.97
142_R 173_L 1.209 0.97
84_L 104_T 1.203 0.96
36_Q 253_S 1.202 0.96
159_V 178_V 1.2 0.96
49_A 282_Q 1.197 0.96
126_L 299_S 1.194 0.96
189_T 192_R 1.191 0.96
141_F 170_L 1.191 0.96
113_E 116_A 1.188 0.96
277_E 306_F 1.186 0.96
145_L 175_L 1.184 0.96
187_E 191_A 1.18 0.96
28_V 60_R 1.175 0.96
164_E 215_A 1.168 0.96
43_M 240_A 1.167 0.96
71_N 360_Q 1.16 0.95
304_P 356_A 1.16 0.95
226_I 280_L 1.16 0.95
57_L 282_Q 1.146 0.95
111_A 151_Q 1.145 0.95
65_T 69_F 1.141 0.95
36_Q 292_P 1.137 0.95
139_D 143_R 1.135 0.95
28_V 31_A 1.134 0.95
203_F 245_W 1.13 0.95
84_L 102_R 1.127 0.94
352_A 355_T 1.116 0.94
206_L 245_W 1.113 0.94
43_M 234_V 1.113 0.94
347_P 351_A 1.111 0.94
107_E 111_A 1.11 0.94
276_D 279_Q 1.108 0.94
266_T 270_E 1.103 0.94
211_R 214_R 1.098 0.93
142_R 172_Q 1.094 0.93
264_P 274_I 1.081 0.93
241_E 290_L 1.077 0.92
43_M 237_Y 1.077 0.92
115_A 152_F 1.072 0.92
202_F 206_L 1.065 0.92
308_D 356_A 1.057 0.92
180_V 208_T 1.057 0.92
175_L 217_I 1.056 0.91
30_R 35_S 1.05 0.91
93_G 265_C 1.048 0.91
59_Q 63_R 1.047 0.91
88_D 95_S 1.045 0.91
207_E 210_D 1.044 0.91
354_L 361_P 1.037 0.91
41_D 253_S 1.035 0.90
96_M 101_K 1.031 0.90
111_A 150_E 1.025 0.90
80_Y 103_K 1.021 0.90
135_K 169_E 1.017 0.89
123_E 363_W 1.005 0.89
50_A 53_Y 1.004 0.89
168_A 215_A 1.003 0.88
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3iixA10.89921000.483Contact Map0.73
3t7vA10.90451000.487Contact Map0.653
1r30A20.89391000.522Contact Map0.663
1oltA10.904599.70.811Contact Map0.471
2qgqA80.740199.70.823Contact Map0.68
4jc0A20.912599.70.827Contact Map0.608
1tv8A20.782599.50.842Contact Map0.645
3rfaA20.867499.40.851Contact Map0.466
2yx0A10.80999.30.857Contact Map0.531
2z2uA10.724199.30.859Contact Map0.621
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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