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OPENSEQ.org
LEU3 - 3-isopropylmalate dehydrogenase
UniProt: P30125 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11577
Length: 363 (356)
Sequences: 3162
Seq/Len: 8.88
LEU3
Paralog alert: 0.65 [within 20: 0.02] - ratio of genomes with paralogs
Cluster includes: DMLA IDH LEU3
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
184_R 218_D 4.833 1.00
177_R 215_E 4.382 1.00
59_P 63_E 3.44 1.00
176_A 209_V 3.428 1.00
25_K 353_D 3.161 1.00
22_Q 352_G 2.96 1.00
228_N 232_Q 2.873 1.00
6_H 39_T 2.845 1.00
328_R 358_Y 2.756 1.00
169_R 173_E 2.639 1.00
177_R 181_E 2.575 1.00
28_D 40_T 2.494 1.00
315_A 318_A 2.469 1.00
310_R 323_E 2.46 1.00
332_E 358_Y 2.427 1.00
354_I 357_R 2.375 1.00
20_M 301_Q 2.317 1.00
55_Q 59_P 2.249 1.00
191_T 222_A 2.248 1.00
353_D 357_R 2.245 1.00
22_Q 25_K 2.212 1.00
34_F 321_A 2.16 1.00
9_V 24_L 2.134 1.00
66_E 102_F 2.126 1.00
310_R 320_C 2.107 1.00
302_I 355_I 2.106 1.00
173_E 211_E 2.093 1.00
44_D 52_N 2.068 1.00
299_I 355_I 2.067 1.00
294_N 347_S 2.039 1.00
24_L 42_H 2.038 1.00
211_E 214_T 2.011 1.00
181_E 216_Y 2.002 1.00
8_A 64_G 1.99 1.00
17_P 21_T 1.99 1.00
173_E 208_I 1.984 1.00
63_E 67_Q 1.973 1.00
340_A 345_A 1.971 1.00
49_A 58_P 1.942 1.00
209_V 221_L 1.935 1.00
176_A 244_L 1.932 1.00
320_C 324_R 1.925 1.00
294_N 349_D 1.918 1.00
329_A 358_Y 1.862 1.00
135_L 182_S 1.852 1.00
193_I 229_A 1.838 1.00
236_D 239_Q 1.837 1.00
306_A 323_E 1.835 1.00
192_S 209_V 1.813 1.00
347_S 350_E 1.808 1.00
212_I 215_E 1.778 1.00
66_E 101_H 1.753 1.00
356_A 359_V 1.744 1.00
303_L 326_I 1.717 1.00
43_Y 64_G 1.703 1.00
27_L 38_I 1.7 1.00
181_E 185_K 1.698 1.00
169_R 211_E 1.694 1.00
15_I 296_A 1.673 1.00
25_K 356_A 1.642 1.00
62_V 101_H 1.63 1.00
316_D 320_C 1.625 1.00
234_I 256_E 1.613 1.00
210_N 223_H 1.609 1.00
65_C 71_V 1.596 1.00
173_E 177_R 1.576 1.00
8_A 68_A 1.572 1.00
352_G 356_A 1.562 1.00
324_R 328_R 1.562 1.00
165_E 246_S 1.557 1.00
294_N 348_T 1.547 1.00
108_L 135_L 1.536 1.00
10_L 45_V 1.519 1.00
208_I 211_E 1.514 1.00
310_R 319_A 1.513 1.00
177_R 216_Y 1.511 1.00
334_I 354_I 1.499 1.00
97_P 101_H 1.49 1.00
190_V 221_L 1.482 1.00
5_Y 315_A 1.452 0.99
204_L 208_I 1.445 0.99
176_A 212_I 1.443 0.99
189_K 222_A 1.437 0.99
189_K 220_E 1.419 0.99
23_A 305_L 1.416 0.99
44_D 53_H 1.408 0.99
57_L 62_V 1.405 0.99
36_M 315_A 1.403 0.99
181_E 184_R 1.388 0.99
36_M 318_A 1.385 0.99
329_A 354_I 1.384 0.99
188_H 241_D 1.381 0.99
29_A 356_A 1.36 0.99
22_Q 294_N 1.335 0.99
8_A 65_C 1.331 0.99
290_I 296_A 1.322 0.99
45_V 98_L 1.316 0.99
196_A 206_R 1.298 0.99
40_T 305_L 1.298 0.99
48_A 52_N 1.297 0.99
192_S 223_H 1.29 0.99
26_V 359_V 1.289 0.99
137_V 178_I 1.284 0.98
137_V 182_S 1.278 0.98
78_G 81_W 1.277 0.98
23_A 301_Q 1.274 0.98
108_L 137_V 1.269 0.98
136_C 254_S 1.267 0.98
131_G 238_S 1.261 0.98
295_I 337_G 1.258 0.98
224_M 228_N 1.251 0.98
125_A 130_N 1.238 0.98
172_I 176_A 1.233 0.98
224_M 232_Q 1.232 0.98
207_E 211_E 1.225 0.98
24_L 28_D 1.221 0.98
169_R 208_I 1.215 0.98
25_K 352_G 1.212 0.98
180_F 190_V 1.209 0.98
44_D 49_A 1.207 0.97
116_G 331_E 1.206 0.97
351_M 355_I 1.205 0.97
115_Q 331_E 1.205 0.97
177_R 212_I 1.198 0.97
280_Y 312_S 1.197 0.97
180_F 219_V 1.195 0.97
112_K 133_D 1.194 0.97
8_A 43_Y 1.194 0.97
41_S 67_Q 1.187 0.97
144_I 165_E 1.186 0.97
112_K 327_N 1.176 0.97
63_E 66_E 1.173 0.97
230_T 253_L 1.168 0.97
187_R 241_D 1.167 0.97
23_A 72_L 1.166 0.97
299_I 351_M 1.165 0.97
232_Q 239_Q 1.164 0.97
41_S 68_A 1.163 0.97
167_Y 246_S 1.158 0.97
30_V 322_I 1.154 0.96
182_S 186_R 1.147 0.96
163_D 201_S 1.147 0.96
60_A 63_E 1.13 0.96
294_N 350_E 1.125 0.96
41_S 64_G 1.119 0.96
180_F 213_A 1.117 0.96
186_R 241_D 1.117 0.96
134_I 233_L 1.115 0.95
144_I 246_S 1.112 0.95
49_A 53_H 1.11 0.95
295_I 345_A 1.109 0.95
224_M 240_F 1.107 0.95
310_R 316_D 1.107 0.95
290_I 337_G 1.106 0.95
73_F 95_L 1.105 0.95
103_K 174_R 1.101 0.95
350_E 353_D 1.098 0.95
165_E 201_S 1.097 0.95
136_C 257_C 1.095 0.95
26_V 322_I 1.095 0.95
346_V 350_E 1.094 0.95
309_L 318_A 1.09 0.95
122_P 127_I 1.088 0.95
334_I 346_V 1.088 0.95
346_V 354_I 1.087 0.95
173_E 212_I 1.086 0.95
328_R 332_E 1.082 0.94
58_P 61_T 1.078 0.94
140_L 271_S 1.071 0.94
273_N 277_F 1.063 0.94
161_A 204_L 1.062 0.94
183_A 190_V 1.058 0.93
222_A 239_Q 1.057 0.93
170_F 173_E 1.048 0.93
106_S 175_I 1.048 0.93
193_I 226_I 1.045 0.93
27_L 305_L 1.042 0.93
72_L 305_L 1.042 0.93
33_R 321_A 1.04 0.93
6_H 41_S 1.036 0.93
53_H 60_A 1.033 0.92
306_A 310_R 1.031 0.92
11_P 42_H 1.028 0.92
184_R 219_V 1.027 0.92
25_K 349_D 1.027 0.92
49_A 61_T 1.022 0.92
305_L 308_L 1.019 0.92
289_D 337_G 1.007 0.91
159_E 169_R 1.007 0.91
19_V 301_Q 1.003 0.91
31_R 36_M 1.001 0.91
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1cnzA20.9891000.044Contact Map0.824
3uduA80.97251000.053Contact Map0.845
3u1hA20.98071000.053Contact Map0.773
3vmkA20.99451000.053Contact Map0.772
1a05A20.97521000.057Contact Map0.782
2y3zA10.96691000.059Contact Map0.739
3r8wA40.99451000.061Contact Map0.765
1vlcA10.9781000.064Contact Map0.758
4iwhA20.96971000.069Contact Map0.791
3flkA40.97521000.072Contact Map0.767
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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