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OPENSEQ.org
EMRA - Multidrug resistance protein A
UniProt: P27303 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11354
Length: 390 (348)
Sequences: 2161
Seq/Len: 6.21
EMRA
Paralog alert: 0.74 [within 20: 0.06] - ratio of genomes with paralogs
Cluster includes: AAEA ACRA ACRE EMRA EMRK MACA MDTA MDTE MDTN YBHG YDHJ YHII YIAV YIBH
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
101_F 206_V 4.951 1.00
84_K 223_T 4.801 1.00
74_K 91_T 4.526 1.00
105_K 210_W 3.892 1.00
266_M 345_V 3.609 1.00
287_G 323_I 3.261 1.00
88_V 217_R 3.207 1.00
108_L 199_V 2.698 1.00
84_K 87_D 2.628 1.00
94_P 98_R 2.609 1.00
266_M 289_V 2.579 1.00
147_V 164_R 2.527 1.00
290_V 324_E 2.52 1.00
129_I 174_L 2.473 1.00
91_T 217_R 2.405 1.00
288_K 326_D 2.348 1.00
85_E 223_T 2.292 1.00
319_L 339_L 2.24 1.00
271_P 286_T 2.172 1.00
130_E 133_K 2.16 1.00
140_Q 171_Q 2.149 1.00
210_W 214_E 2.111 1.00
102_E 105_K 2.081 1.00
129_I 180_Q 2.063 1.00
59_G 253_W 2.034 1.00
150_G 160_L 2.021 1.00
207_R 210_W 1.993 1.00
81_D 87_D 1.927 1.00
58_A 255_D 1.923 1.00
273_T 284_K 1.916 1.00
208_N 212_A 1.914 1.00
123_K 126_Q 1.884 1.00
288_K 324_E 1.871 1.00
220_S 246_V 1.861 1.00
200_Q 203_A 1.817 1.00
258_F 266_M 1.804 1.00
143_Y 167_V 1.781 1.00
258_F 345_V 1.748 1.00
76_W 91_T 1.747 1.00
167_V 171_Q 1.673 1.00
98_R 102_E 1.672 1.00
136_L 167_V 1.641 1.00
274_I 341_T 1.615 1.00
101_F 210_W 1.606 1.00
138_K 142_D 1.605 1.00
272_V 343_V 1.601 1.00
147_V 151_N 1.6 1.00
274_I 343_V 1.59 0.99
108_L 206_V 1.587 0.99
146_R 155_I 1.566 0.99
276_T 341_T 1.565 0.99
143_Y 147_V 1.56 0.99
136_L 140_Q 1.547 0.99
98_R 213_L 1.546 0.99
82_F 225_Y 1.545 0.99
274_I 325_L 1.544 0.99
211_L 215_R 1.539 0.99
90_V 243_L 1.539 0.99
88_V 219_I 1.536 0.99
90_V 218_I 1.529 0.99
144_N 147_V 1.503 0.99
112_V 203_A 1.498 0.99
140_Q 144_N 1.497 0.99
286_T 326_D 1.497 0.99
80_T 291_G 1.495 0.99
254_V 341_T 1.479 0.99
146_R 163_A 1.476 0.99
143_Y 164_R 1.475 0.99
100_A 208_N 1.474 0.99
67_Q 215_R 1.472 0.99
184_N 187_M 1.468 0.99
106_T 204_T 1.437 0.99
272_V 287_G 1.437 0.99
60_N 253_W 1.431 0.99
164_R 168_T 1.426 0.99
64_I 244_M 1.408 0.98
212_A 215_R 1.401 0.98
72_V 231_V 1.401 0.98
178_I 181_Y 1.398 0.98
76_W 88_V 1.38 0.98
97_A 216_T 1.377 0.98
258_F 343_V 1.375 0.98
272_V 323_I 1.364 0.98
42_W 47_R 1.356 0.98
50_E 266_M 1.35 0.98
122_S 180_Q 1.349 0.98
160_L 164_R 1.341 0.98
180_Q 184_N 1.336 0.98
70_G 92_L 1.332 0.98
100_A 212_A 1.324 0.97
58_A 299_A 1.321 0.97
271_P 288_K 1.317 0.97
237_I 243_L 1.292 0.97
133_K 178_I 1.285 0.97
73_T 93_D 1.284 0.97
157_R 161_Q 1.282 0.97
36_V 39_G 1.282 0.97
126_Q 184_N 1.24 0.96
105_K 206_V 1.239 0.96
103_K 205_E 1.227 0.95
67_Q 71_S 1.225 0.95
256_A 323_I 1.223 0.95
143_Y 160_L 1.222 0.95
140_Q 167_V 1.211 0.95
101_F 105_K 1.207 0.95
36_V 40_I 1.201 0.95
137_A 140_Q 1.193 0.94
24_L 28_T 1.189 0.94
25_L 29_L 1.178 0.94
62_I 222_M 1.176 0.94
133_K 137_A 1.168 0.94
135_A 138_K 1.159 0.93
107_A 201_Q 1.156 0.93
66_S 237_I 1.152 0.93
42_W 48_H 1.146 0.93
146_R 158_E 1.144 0.93
254_V 274_I 1.136 0.92
275_T 344_S 1.12 0.92
168_T 171_Q 1.111 0.91
273_T 286_T 1.106 0.91
72_V 90_V 1.106 0.91
26_L 30_L 1.102 0.91
296_T 319_L 1.101 0.91
74_K 233_P 1.101 0.91
214_E 219_I 1.094 0.90
115_T 199_V 1.093 0.90
102_E 207_R 1.088 0.90
134_I 165_D 1.088 0.90
119_M 196_Q 1.087 0.90
69_S 236_Q 1.08 0.90
82_F 223_T 1.078 0.90
106_T 197_P 1.076 0.89
107_A 205_E 1.073 0.89
49_F 344_S 1.063 0.89
101_F 213_L 1.063 0.89
94_P 217_R 1.06 0.88
50_E 262_Q 1.055 0.88
312_W 337_I 1.055 0.88
23_L 26_L 1.053 0.88
75_V 233_P 1.052 0.88
181_Y 184_N 1.049 0.88
41_Y 45_V 1.044 0.87
132_Q 135_A 1.042 0.87
78_D 81_D 1.042 0.87
290_V 322_R 1.037 0.87
132_Q 177_A 1.03 0.86
34_I 38_I 1.029 0.86
225_Y 336_R 1.025 0.86
306_Q 312_W 1.022 0.86
122_S 129_I 1.017 0.85
178_I 184_N 1.016 0.85
298_S 305_A 1.013 0.85
268_I 271_P 1.012 0.85
75_V 232_Q 1.008 0.85
307_N 311_N 1 0.84
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4dk0A10.83081000.461Contact Map0.6
3fppA20.79231000.466Contact Map0.727
3lnnA20.81031000.506Contact Map0.663
1vf7A130.74621000.516Contact Map0.726
4l8jA10.72821000.53Contact Map0.687
3ne5B20.72051000.54Contact Map0.701
2f1mA40.66921000.551Contact Map0.652
4kkuA40.62561000.597Contact Map0.74
2k32A10.294999.90.76Contact Map0.708
2dneA10.25999.60.817Contact Map0.586
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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