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OPENSEQ.org
GCST - Aminomethyltransferase
UniProt: P27248 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11442
Length: 364 (355)
Sequences: 3352
Seq/Len: 9.44
GCST
Paralog alert: 0.58 [within 20: 0.15] - ratio of genomes with paralogs
Cluster includes: GCST YGFZ
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
10_Q 209_R 3.844 1.00
302_D 305_G 3.393 1.00
297_P 308_H 3.277 1.00
285_V 323_Y 3.182 1.00
298_V 328_A 3.114 1.00
245_S 273_Q 3.089 1.00
272_V 276_H 3 1.00
284_L 326_A 2.959 1.00
38_H 42_T 2.806 1.00
339_I 346_E 2.666 1.00
151_N 158_T 2.662 1.00
12_T 18_M 2.614 1.00
8_Y 12_T 2.563 1.00
143_S 204_A 2.54 1.00
337_T 348_P 2.534 1.00
44_A 159_L 2.462 1.00
295_E 311_I 2.451 1.00
43_D 151_N 2.387 1.00
238_Q 294_N 2.356 1.00
341_Q 344_N 2.347 1.00
285_V 350_K 2.331 1.00
338_A 349_V 2.298 1.00
45_G 219_C 2.25 1.00
337_T 350_K 2.204 1.00
318_S 321_L 2.2 1.00
56_D 108_F 2.199 1.00
13_L 209_R 2.187 1.00
61_R 64_E 2.158 1.00
9_E 13_L 2.084 1.00
9_E 12_T 2.073 1.00
40_V 45_G 2.05 1.00
287_T 350_K 2.036 1.00
341_Q 346_E 2.007 1.00
69_L 125_I 1.977 1.00
312_I 326_A 1.961 1.00
63_R 109_F 1.943 1.00
67_R 73_D 1.934 1.00
55_V 122_L 1.911 1.00
39_A 43_D 1.902 1.00
46_M 145_I 1.897 1.00
80_S 103_Y 1.888 1.00
58_R 108_F 1.881 1.00
124_W 128_H 1.86 1.00
10_Q 13_L 1.855 1.00
299_R 346_E 1.821 1.00
44_A 155_K 1.806 1.00
83_A 100_I 1.799 1.00
10_Q 14_C 1.797 1.00
4_Q 25_M 1.795 1.00
167_A 170_G 1.792 1.00
267_R 271_E 1.789 1.00
282_V 330_V 1.772 1.00
330_V 338_A 1.771 1.00
38_H 320_T 1.737 1.00
339_I 348_P 1.716 1.00
143_S 201_N 1.71 1.00
298_V 340_V 1.709 1.00
263_D 267_R 1.699 1.00
11_H 30_Y 1.692 1.00
6_P 143_S 1.69 1.00
200_P 203_K 1.67 1.00
205_A 209_R 1.637 1.00
309_E 332_E 1.635 1.00
9_E 205_A 1.631 1.00
39_A 217_K 1.631 1.00
284_L 349_V 1.622 1.00
124_W 265_I 1.619 1.00
300_F 307_Q 1.607 1.00
60_S 133_G 1.583 1.00
58_R 135_E 1.579 1.00
307_Q 331_P 1.57 1.00
208_W 218_P 1.531 1.00
58_R 137_T 1.53 1.00
80_S 106_E 1.525 1.00
284_L 328_A 1.524 1.00
268_E 271_E 1.518 1.00
57_L 111_L 1.516 1.00
199_L 207_F 1.513 1.00
74_V 101_V 1.511 1.00
203_K 206_D 1.505 1.00
244_I 329_R 1.496 1.00
4_Q 8_Y 1.495 1.00
37_H 226_T 1.486 1.00
61_R 133_G 1.482 1.00
71_A 89_L 1.48 1.00
120_K 242_E 1.457 1.00
87_G 254_T 1.455 1.00
119_E 123_S 1.432 1.00
87_G 251_M 1.421 0.99
62_T 109_F 1.398 0.99
87_G 95_V 1.394 0.99
263_D 268_E 1.376 0.99
210_A 213_E 1.361 0.99
111_L 125_I 1.358 0.99
56_D 137_T 1.357 0.99
241_D 244_I 1.353 0.99
151_N 154_A 1.336 0.99
105_T 108_F 1.334 0.99
299_R 341_Q 1.329 0.99
247_L 257_W 1.315 0.99
103_Y 106_E 1.315 0.99
178_Q 181_D 1.313 0.99
14_C 218_P 1.309 0.99
297_P 341_Q 1.301 0.99
57_L 62_T 1.299 0.99
16_A 26_M 1.299 0.99
55_V 113_V 1.295 0.99
56_D 139_R 1.29 0.99
63_R 75_A 1.285 0.99
179_A 184_I 1.282 0.99
10_Q 205_A 1.281 0.99
4_Q 9_E 1.279 0.99
57_L 125_I 1.274 0.99
43_D 217_K 1.268 0.99
95_V 240_M 1.263 0.98
172_K 176_G 1.254 0.98
46_M 212_V 1.245 0.98
67_R 75_A 1.242 0.98
70_L 101_V 1.241 0.98
69_L 128_H 1.233 0.98
6_P 201_N 1.233 0.98
284_L 340_V 1.223 0.98
309_E 338_A 1.211 0.98
70_L 86_S 1.209 0.98
24_W 118_R 1.206 0.98
82_K 176_G 1.205 0.98
206_D 210_A 1.193 0.98
269_A 273_Q 1.184 0.97
102_Y 177_V 1.183 0.97
299_R 308_H 1.175 0.97
302_D 307_Q 1.173 0.97
63_R 103_Y 1.164 0.97
286_M 349_V 1.161 0.97
103_Y 109_F 1.156 0.97
268_E 272_V 1.153 0.97
122_L 136_I 1.153 0.97
274_R 277_G 1.152 0.97
139_R 142_L 1.152 0.97
19_V 28_L 1.146 0.97
327_L 354_P 1.139 0.97
8_Y 25_M 1.137 0.96
342_I 347_M 1.136 0.96
32_S 35_D 1.136 0.96
123_S 127_Q 1.129 0.96
317_F 322_G 1.127 0.96
29_H 33_Q 1.125 0.96
249_A 329_R 1.125 0.96
247_L 270_L 1.121 0.96
5_T 8_Y 1.114 0.96
122_L 126_T 1.113 0.96
228_R 314_S 1.108 0.96
164_Q 168_V 1.099 0.96
336_E 351_V 1.091 0.95
229_L 314_S 1.091 0.95
53_T 118_R 1.078 0.95
33_Q 222_G 1.078 0.95
247_L 278_T 1.077 0.95
101_V 111_L 1.076 0.95
7_L 145_I 1.074 0.95
245_S 248_A 1.073 0.95
65_F 134_I 1.072 0.95
46_M 208_W 1.059 0.94
234_N 240_M 1.046 0.94
229_L 316_T 1.032 0.93
184_I 195_Y 1.03 0.93
88_M 125_I 1.027 0.93
332_E 336_E 1.023 0.93
68_Y 265_I 1.019 0.93
257_W 271_E 1.016 0.92
35_D 38_H 1.013 0.92
330_V 351_V 1.01 0.92
35_D 217_K 1.009 0.92
78_T 82_K 1.008 0.92
147_V 156_A 1.008 0.92
257_W 274_R 1.007 0.92
288_E 348_P 1.006 0.92
57_L 65_F 1.003 0.92
164_Q 167_A 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1yx2A20.95331000.045Contact Map0.852
1vloA10.98351000.045Contact Map0.779
3girA10.96151000.049Contact Map0.748
1wosA10.95331000.06Contact Map0.82
2gagA10.9781000.061Contact Map0.766
1pj5A10.9561000.064Contact Map0.807
1v5vA20.96431000.064Contact Map0.842
1wsrA20.96431000.069Contact Map0.781
3tfiA20.93681000.101Contact Map0.731
3ttgA10.87641000.158Contact Map0.682
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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