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OPENSEQ.org
RFAI - Lipopolysaccharide 1,3-galactosyltransferase
UniProt: P27128 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11352
Length: 339 (300)
Sequences: 442
Seq/Len: 1.47
RFAI
Paralog alert: 0.67 [within 20: 0.26] - ratio of genomes with paralogs
Cluster includes: RFAI RFAJ
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
110_I 184_S 5.408 1.00
33_Y 46_S 3.884 1.00
37_K 72_D 2.91 1.00
184_S 219_P 2.872 1.00
62_H 90_Y 2.823 1.00
125_P 192_A 2.705 1.00
110_I 219_P 2.705 1.00
42_G 286_F 2.59 1.00
191_T 195_A 2.587 1.00
128_L 188_L 2.428 1.00
53_Y 289_A 2.415 1.00
47_I 61_F 2.374 1.00
156_V 182_F 2.357 1.00
126_K 190_N 2.332 0.99
30_D 62_H 2.313 0.99
189_I 194_W 2.258 0.99
45_I 135_I 2.137 0.99
60_C 88_K 2.07 0.98
182_F 224_L 2.056 0.98
50_I 61_F 1.905 0.97
30_D 60_C 1.871 0.97
197_Q 231_K 1.855 0.96
135_I 266_Y 1.852 0.96
148_F 152_K 1.82 0.96
32_A 116_I 1.807 0.96
121_I 195_A 1.807 0.96
193_Q 196_A 1.778 0.95
136_C 263_F 1.771 0.95
44_G 295_W 1.74 0.94
118_D 200_S 1.704 0.93
32_A 127_V 1.679 0.93
130_L 186_F 1.662 0.92
198_Q 201_A 1.658 0.92
63_I 89_I 1.632 0.91
106_W 219_P 1.627 0.91
134_I 186_F 1.622 0.91
201_A 205_A 1.593 0.90
152_K 190_N 1.548 0.88
138_G 262_I 1.511 0.86
181_Y 222_D 1.511 0.86
35_T 132_A 1.494 0.85
192_A 196_A 1.472 0.84
35_T 43_C 1.459 0.84
239_Y 262_I 1.44 0.82
54_N 139_T 1.415 0.81
206_M 226_M 1.398 0.80
186_F 263_F 1.389 0.79
181_Y 286_F 1.38 0.79
124_A 191_T 1.361 0.77
36_D 109_A 1.36 0.77
46_S 134_I 1.353 0.77
257_V 261_T 1.331 0.75
151_D 190_N 1.329 0.75
149_P 233_I 1.315 0.74
128_L 144_I 1.312 0.74
50_I 140_I 1.309 0.73
148_F 190_N 1.304 0.73
121_I 191_T 1.302 0.73
156_V 224_L 1.301 0.73
113_R 220_D 1.296 0.72
223_V 227_L 1.295 0.72
153_V 190_N 1.285 0.71
137_Q 285_A 1.269 0.70
137_Q 264_I 1.265 0.70
33_Y 50_I 1.26 0.69
129_Y 220_D 1.252 0.68
220_D 225_N 1.247 0.68
153_V 231_K 1.239 0.67
203_A 223_V 1.236 0.67
184_S 224_L 1.225 0.66
199_V 202_R 1.216 0.65
222_D 286_F 1.208 0.64
45_I 290_K 1.202 0.64
310_R 313_A 1.198 0.64
111_Y 172_L 1.192 0.63
148_F 155_M 1.167 0.60
181_Y 225_N 1.166 0.60
147_S 152_K 1.155 0.59
143_L 235_A 1.147 0.59
97_L 115_V 1.146 0.58
130_L 136_C 1.145 0.58
135_I 264_I 1.145 0.58
50_I 136_C 1.141 0.58
287_M 290_K 1.138 0.58
184_S 220_D 1.128 0.57
56_G 140_I 1.128 0.57
319_K 322_Y 1.127 0.57
194_W 199_V 1.123 0.56
30_D 124_A 1.122 0.56
238_K 262_I 1.12 0.56
190_N 193_Q 1.118 0.56
44_G 293_S 1.109 0.55
103_T 159_E 1.106 0.54
220_D 240_N 1.101 0.54
223_V 228_L 1.099 0.54
48_A 83_Y 1.096 0.53
114_F 203_A 1.086 0.52
306_S 318_K 1.069 0.51
181_Y 220_D 1.068 0.50
253_F 268_G 1.066 0.50
138_G 238_K 1.063 0.50
30_D 139_T 1.056 0.49
39_F 44_G 1.053 0.49
121_I 124_A 1.047 0.48
37_K 69_G 1.045 0.48
146_F 233_I 1.041 0.48
152_K 155_M 1.04 0.48
83_Y 266_Y 1.038 0.47
153_V 228_L 1.037 0.47
47_I 50_I 1.034 0.47
307_N 311_Y 1.029 0.46
51_L 85_T 1.028 0.46
111_Y 182_F 1.025 0.46
37_K 40_L 1.018 0.45
316_M 324_K 1.01 0.45
107_T 219_P 1.008 0.44
308_Q 316_M 1.007 0.44
59_L 124_A 1.007 0.44
112_F 271_K 1.001 0.44
324_K 327_S 1 0.44
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1g9rA10.85841000.42Contact Map0.719
3tztA20.79651000.444Contact Map0.713
3u2uA20.70211000.602Contact Map0.514
1ll2A10.79651000.615Contact Map0.529
2p6wA10.598893.10.942Contact Map0.384
3iohA10.734590.60.946Contact Map0.494
1o7qA20.7168890.948Contact Map0.482
1xhbA10.8142820.952Contact Map0.649
4aylA10.64958.50.96Contact Map0.47
2d7iA10.852546.20.963Contact Map0.662
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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