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OPENSEQ.org
RFAB - Lipopolysaccharide 1,6-galactosyltransferase
UniProt: P27127 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11351
Length: 359 (351)
Sequences: 19881
Seq/Len: 56.64
RFAB
Paralog alert: 0.87 [within 20: 0.44] - ratio of genomes with paralogs
Cluster includes: GLGA RFAB RFAG WBBK WCAC WCAI WCAL
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
182_I 256_V 4.233 1.00
210_K 237_V 3.574 1.00
220_F 224_Q 3.187 1.00
2_K 33_Q 3.061 1.00
89_I 351_I 3.036 1.00
134_Y 157_N 3.028 1.00
200_D 226_Y 2.88 1.00
204_Q 313_V 2.423 1.00
180_T 212_H 2.4 1.00
259_L 275_A 2.38 1.00
178_T 210_K 2.356 1.00
35_E 85_P 2.341 1.00
202_L 211_L 2.334 1.00
204_Q 310_A 2.277 1.00
183_Y 195_V 2.244 1.00
143_K 147_I 2.241 1.00
286_D 292_A 2.175 1.00
212_H 239_Y 2.156 1.00
35_E 56_S 2.151 1.00
89_I 115_W 2.095 1.00
179_A 320_I 2.093 1.00
286_D 305_Q 2.066 1.00
295_I 301_G 2.017 1.00
305_Q 311_G 2.015 1.00
30_Q 345_D 1.998 1.00
200_D 309_I 1.986 1.00
35_E 83_Y 1.962 1.00
194_R 197_D 1.959 1.00
4_A 85_P 1.955 1.00
180_T 255_K 1.915 1.00
181_F 258_A 1.903 1.00
296_Q 299_V 1.886 1.00
32_I 348_H 1.871 1.00
216_D 220_F 1.853 1.00
226_Y 230_L 1.85 1.00
214_L 252_D 1.848 1.00
302_H 315_L 1.841 1.00
114_S 132_A 1.831 1.00
4_A 37_F 1.829 1.00
322_G 325_H 1.798 1.00
315_L 318_K 1.776 1.00
206_K 235_R 1.774 1.00
318_K 325_H 1.762 1.00
334_S 337_E 1.755 1.00
140_S 143_K 1.749 1.00
198_L 260_L 1.746 1.00
308_D 311_G 1.735 1.00
342_K 346_R 1.734 1.00
228_R 233_D 1.72 1.00
181_F 316_L 1.72 1.00
219_D 222_K 1.713 1.00
227_G 238_W 1.695 1.00
26_T 29_Q 1.665 1.00
180_T 210_K 1.656 1.00
212_H 237_V 1.651 1.00
309_I 313_V 1.647 1.00
194_R 264_S 1.644 1.00
284_S 303_L 1.639 1.00
140_S 293_D 1.621 1.00
205_A 317_N 1.618 1.00
187_M 213_V 1.602 1.00
259_L 280_I 1.602 1.00
179_A 257_S 1.601 1.00
311_G 315_L 1.6 1.00
227_G 232_I 1.598 1.00
318_K 322_G 1.598 1.00
198_L 211_L 1.593 1.00
143_K 158_V 1.564 1.00
203_S 230_L 1.555 1.00
225_A 229_E 1.538 1.00
272_L 291_P 1.518 1.00
296_Q 300_N 1.495 1.00
3_I 32_I 1.49 1.00
140_S 144_Q 1.48 1.00
140_S 158_V 1.48 1.00
5_F 91_I 1.479 1.00
162_P 340_Q 1.478 1.00
143_K 156_I 1.454 1.00
30_Q 348_H 1.437 1.00
181_F 260_L 1.429 1.00
26_T 344_Y 1.422 1.00
202_L 209_W 1.415 1.00
304_Y 315_L 1.411 1.00
137_A 156_I 1.402 0.99
317_N 321_A 1.4 0.99
1_M 32_I 1.385 0.99
325_H 328_H 1.379 0.99
196_K 226_Y 1.378 0.99
35_E 54_K 1.369 0.99
201_G 312_F 1.367 0.99
26_T 341_S 1.367 0.99
214_L 239_Y 1.363 0.99
242_Q 252_D 1.361 0.99
302_H 318_K 1.357 0.99
5_F 27_F 1.349 0.99
226_Y 229_E 1.339 0.99
152_A 155_T 1.338 0.99
333_A 336_D 1.337 0.99
134_Y 350_V 1.336 0.99
279_G 332_P 1.334 0.99
256_V 280_I 1.332 0.99
346_R 349_K 1.317 0.99
21_R 25_T 1.313 0.99
157_N 353_S 1.31 0.99
195_V 213_V 1.309 0.99
332_P 335_I 1.303 0.99
299_V 330_K 1.3 0.99
192_Q 265_F 1.299 0.99
196_K 264_S 1.298 0.99
136_L 350_V 1.296 0.99
2_K 35_E 1.295 0.99
284_S 291_P 1.294 0.99
342_K 345_D 1.279 0.99
168_S 278_W 1.266 0.99
147_I 153_E 1.264 0.99
214_L 242_Q 1.254 0.99
90_C 112_V 1.245 0.98
184_V 253_I 1.243 0.98
323_E 326_I 1.241 0.98
182_I 214_L 1.241 0.98
180_T 257_S 1.238 0.98
197_D 309_I 1.215 0.98
268_F 273_L 1.211 0.98
204_Q 309_I 1.206 0.98
284_S 295_I 1.205 0.98
311_G 314_T 1.198 0.98
285_A 304_Y 1.194 0.98
348_H 352_I 1.192 0.98
175_E 178_T 1.184 0.98
289_S 292_A 1.183 0.98
144_Q 147_I 1.18 0.98
221_E 224_Q 1.178 0.98
136_L 347_L 1.174 0.98
144_Q 148_N 1.174 0.98
283_I 304_Y 1.174 0.98
188_K 192_Q 1.173 0.98
5_F 24_I 1.172 0.98
305_Q 308_D 1.172 0.98
137_A 143_K 1.17 0.97
117_H 270_M 1.162 0.97
184_V 214_L 1.161 0.97
205_A 209_W 1.158 0.97
179_A 258_A 1.157 0.97
138_I 161_N 1.157 0.97
339_Y 342_K 1.154 0.97
242_Q 248_L 1.148 0.97
28_R 31_H 1.147 0.97
263_S 266_E 1.143 0.97
248_L 251_Q 1.14 0.97
261_L 271_T 1.135 0.97
243_Q 248_L 1.135 0.97
269_P 291_P 1.115 0.96
160_F 293_D 1.115 0.96
197_D 307_G 1.109 0.96
186_R 192_Q 1.098 0.96
212_H 252_D 1.092 0.96
330_K 333_A 1.089 0.96
300_N 334_S 1.085 0.96
137_A 146_M 1.083 0.96
212_H 256_V 1.083 0.96
183_Y 198_L 1.08 0.95
197_D 264_S 1.079 0.95
199_L 227_G 1.077 0.95
14_G 17_E 1.072 0.95
239_Y 242_Q 1.072 0.95
182_I 253_I 1.052 0.95
19_V 270_M 1.052 0.95
24_I 36_M 1.047 0.94
276_L 282_C 1.047 0.94
275_A 280_I 1.045 0.94
224_Q 228_R 1.043 0.94
136_L 159_I 1.038 0.94
295_I 300_N 1.035 0.94
196_K 200_D 1.034 0.94
111_P 133_D 1.032 0.94
345_D 349_K 1.023 0.93
297_P 315_L 1.017 0.93
340_Q 343_Y 1.014 0.93
184_V 249_V 1.01 0.93
253_I 278_W 1.007 0.93
137_A 142_I 1.004 0.93
164_E 167_D 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2r60A10.98051000.109Contact Map0.704
1rzuA20.97211000.13Contact Map0.716
2qzsA10.96941000.134Contact Map0.746
3c48A20.98051000.14Contact Map0.819
3froA30.97491000.157Contact Map0.778
2x0dA20.93591000.173Contact Map0.623
3s28A80.98611000.177Contact Map0.607
2gekA10.96381000.177Contact Map0.715
2jjmA120.97771000.183Contact Map0.801
3oy2A20.96661000.184Contact Map0.609
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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