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OPENSEQ.org
LEP4 - Type 4 prepilin-like proteins leader peptide-processing enzyme
UniProt: P25960 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11359
Length: 225 (219)
Sequences: 916
Seq/Len: 4.18
LEP4
Paralog alert: 0.08 [within 20: 0.00] - ratio of genomes with paralogs
Cluster includes: LEP4 PPPA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
108_L 164_A 3.174 1.00
90_L 100_L 2.921 1.00
15_A 19_V 2.709 1.00
156_Y 159_F 2.683 1.00
13_F 57_L 2.608 1.00
39_Q 42_V 2.514 1.00
165_A 177_I 2.51 1.00
23_A 92_V 2.497 1.00
39_Q 48_K 2.413 1.00
69_L 96_R 2.393 1.00
49_Y 54_T 2.384 1.00
100_L 105_T 2.203 1.00
102_D 160_K 2.18 1.00
91_S 210_A 2.146 1.00
9_I 78_G 2.081 1.00
186_I 189_A 1.994 1.00
35_L 38_R 1.932 1.00
34_A 38_R 1.86 1.00
22_I 26_L 1.849 1.00
86_F 104_L 1.815 1.00
100_L 206_G 1.811 1.00
177_I 212_G 1.797 1.00
145_V 149_C 1.794 1.00
165_A 213_S 1.755 0.99
42_V 45_R 1.752 0.99
53_D 63_A 1.744 0.99
100_L 161_L 1.73 0.99
40_V 43_H 1.728 0.99
47_K 53_D 1.691 0.99
112_G 168_W 1.682 0.99
131_A 167_A 1.67 0.99
40_V 45_R 1.611 0.99
145_V 153_G 1.593 0.99
12_G 16_D 1.592 0.99
212_G 216_N 1.586 0.99
77_T 81_F 1.558 0.98
70_A 74_P 1.557 0.98
134_G 174_L 1.506 0.98
53_D 59_L 1.503 0.98
161_L 206_G 1.492 0.98
20_N 23_A 1.486 0.98
181_A 209_L 1.481 0.98
38_R 41_L 1.48 0.98
38_R 42_V 1.476 0.97
63_A 66_A 1.461 0.97
50_A 53_D 1.458 0.97
47_K 50_A 1.456 0.97
88_L 92_V 1.443 0.97
87_V 210_A 1.416 0.97
17_Y 25_H 1.402 0.96
130_G 166_G 1.389 0.96
34_A 41_L 1.385 0.96
92_V 96_R 1.379 0.96
34_A 37_F 1.366 0.96
101_P 160_K 1.363 0.95
34_A 42_V 1.341 0.95
49_Y 52_H 1.337 0.95
18_F 25_H 1.322 0.94
101_P 104_L 1.301 0.94
33_T 42_V 1.295 0.94
84_F 88_L 1.289 0.93
51_W 54_T 1.267 0.93
13_F 78_G 1.257 0.92
67_C 74_P 1.252 0.92
23_A 93_I 1.245 0.92
9_I 13_F 1.23 0.91
66_A 74_P 1.23 0.91
186_I 192_S 1.225 0.91
19_V 89_T 1.216 0.90
162_L 177_I 1.211 0.90
149_C 153_G 1.202 0.90
34_A 40_V 1.2 0.90
69_L 73_T 1.2 0.90
17_Y 21_A 1.196 0.89
142_Y 152_E 1.186 0.89
156_Y 160_K 1.175 0.88
63_A 67_C 1.173 0.88
70_A 73_T 1.171 0.88
37_F 42_V 1.168 0.88
90_L 101_P 1.168 0.88
84_F 214_M 1.167 0.88
58_I 62_A 1.149 0.87
20_N 24_Y 1.142 0.86
30_E 41_L 1.117 0.85
21_A 27_S 1.116 0.84
184_G 211_L 1.111 0.84
33_T 37_F 1.111 0.84
162_L 209_L 1.082 0.82
90_L 105_T 1.072 0.81
69_L 92_V 1.062 0.80
135_Y 162_L 1.059 0.80
185_G 189_A 1.053 0.79
16_D 25_H 1.037 0.78
130_G 206_G 1.037 0.78
138_L 178_L 1.035 0.78
139_W 143_W 1.031 0.77
80_L 84_F 1.03 0.77
73_T 77_T 1.027 0.77
135_Y 159_F 1.024 0.77
35_L 45_R 1.021 0.76
65_I 74_P 1.018 0.76
182_S 186_I 1.017 0.76
19_V 22_I 1.014 0.76
129_Y 133_A 1.005 0.75
49_Y 53_D 1.001 0.74
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3s0xA20.657899.30.753Contact Map0.456
1iycA10.12444.50.96Contact Map0.113
2k5cA10.30223.50.961Contact Map0.226
2jvxA10.12442.30.965Contact Map0.316
2bayA60.20892.10.966Contact Map0.359
3phfA160.328920.966Contact Map0.081
1lv3A10.284420.966Contact Map0.192
3t58A40.31111.70.967Contact Map0.372
2k1eA40.42221.20.97Contact Map0.709
4gxbB10.16441.20.971Contact Map0.135
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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