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OPENSEQ.org
RHLE - ATP-dependent RNA helicase RhlE
UniProt: P25888 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11235
Length: 454 (377)
Sequences: 9512
Seq/Len: 25.23
RHLE
Paralog alert: 0.86 [within 20: 0.08] - ratio of genomes with paralogs
Cluster includes: DBPA DEAD RHLB RHLE SRMB
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
56_T 95_V 5.085 1.00
59_L 78_A 4.255 1.00
9_S 61_Q 4.219 1.00
131_A 136_L 4.002 1.00
35_V 184_L 3.864 1.00
27_I 43_A 3.677 1.00
82_T 91_I 3.651 1.00
39_R 206_L 3.199 1.00
139_L 144_A 3.013 1.00
252_T 258_A 2.939 1.00
18_E 101_Y 2.872 1.00
59_L 154_V 2.747 1.00
289_A 293_S 2.662 1.00
255_K 278_N 2.585 1.00
10_P 14_R 2.52 1.00
322_L 347_S 2.47 1.00
313_E 341_A 2.45 1.00
18_E 100_K 2.446 1.00
290_D 295_D 2.424 1.00
153_L 174_L 2.424 1.00
141_H 172_R 2.407 1.00
222_Q 333_I 2.395 1.00
21_Y 53_A 2.362 1.00
273_A 296_I 2.326 1.00
94_N 97_D 2.317 1.00
333_I 345_A 2.314 1.00
92_G 106_S 2.298 1.00
4_D 17_A 2.199 1.00
316_H 346_L 2.198 1.00
171_R 200_K 2.194 1.00
270_I 298_V 2.159 1.00
222_Q 329_Y 2.159 1.00
92_G 96_R 2.127 1.00
79_L 150_V 2.038 1.00
15_A 101_Y 2.006 1.00
262_A 272_S 1.974 1.00
12_I 61_Q 1.974 1.00
107_L 126_V 1.973 1.00
221_T 344_E 1.966 1.00
14_R 101_Y 1.953 1.00
160_R 163_D 1.945 1.00
81_L 136_L 1.932 1.00
77_R 127_D 1.879 1.00
195_K 207_E 1.846 1.00
305_A 309_L 1.833 1.00
252_T 300_V 1.803 1.00
95_V 106_S 1.781 1.00
19_Q 97_D 1.776 1.00
34_A 206_L 1.774 1.00
116_N 120_M 1.769 1.00
137_L 172_R 1.766 1.00
95_V 128_V 1.765 1.00
258_A 276_H 1.764 1.00
237_S 268_D 1.747 1.00
80_I 95_V 1.744 1.00
140_E 176_K 1.731 1.00
77_R 151_E 1.73 1.00
257_G 321_E 1.716 1.00
92_G 108_V 1.696 1.00
167_I 171_R 1.691 1.00
223_H 344_E 1.691 1.00
319_N 347_S 1.689 1.00
59_L 152_I 1.685 1.00
38_G 182_Q 1.654 1.00
219_Q 342_T 1.649 1.00
8_L 57_L 1.641 1.00
34_A 37_E 1.641 1.00
220_V 337_G 1.641 1.00
62_H 151_E 1.64 1.00
55_F 91_I 1.64 1.00
62_H 152_I 1.618 1.00
286_R 290_D 1.601 1.00
271_R 297_R 1.59 1.00
217_S 220_V 1.574 1.00
262_A 274_A 1.563 1.00
43_A 186_F 1.561 1.00
259_N 274_A 1.551 1.00
258_A 274_A 1.545 1.00
88_A 108_V 1.543 1.00
301_A 305_A 1.541 1.00
14_R 18_E 1.539 1.00
40_D 203_H 1.537 1.00
273_A 287_A 1.533 1.00
323_P 329_Y 1.525 1.00
138_D 142_Q 1.514 1.00
319_N 333_I 1.511 1.00
248_V 318_V 1.5 1.00
16_V 21_Y 1.498 1.00
75_P 149_Q 1.496 1.00
250_V 298_V 1.48 1.00
252_T 261_L 1.47 1.00
195_K 199_E 1.465 1.00
16_V 53_A 1.447 1.00
89_A 285_T 1.44 1.00
131_A 145_V 1.436 1.00
232_K 321_E 1.42 1.00
265_L 300_V 1.414 1.00
95_V 130_V 1.41 1.00
233_R 264_Q 1.407 0.99
34_A 208_I 1.407 0.99
239_M 346_L 1.401 0.99
120_M 124_G 1.398 0.99
2_S 5_S 1.386 0.99
273_A 290_D 1.379 0.99
7_G 13_L 1.374 0.99
81_L 131_A 1.373 0.99
361_E 367_E 1.372 0.99
147_L 150_V 1.311 0.99
55_F 82_T 1.303 0.99
119_M 139_L 1.291 0.99
119_M 144_A 1.28 0.99
107_L 129_L 1.275 0.99
39_R 204_N 1.26 0.99
291_F 299_L 1.258 0.99
248_V 298_V 1.255 0.99
221_T 342_T 1.252 0.99
221_T 369_P 1.238 0.98
107_L 121_K 1.234 0.98
17_A 22_R 1.231 0.98
60_L 102_L 1.23 0.98
82_T 88_A 1.227 0.98
322_L 360_I 1.227 0.98
60_L 99_S 1.211 0.98
247_Q 313_E 1.209 0.98
114_S 117_P 1.2 0.98
35_V 41_L 1.197 0.98
126_V 129_L 1.194 0.98
279_K 287_A 1.183 0.98
109_V 129_L 1.181 0.98
40_D 202_L 1.179 0.98
137_L 169_D 1.177 0.98
261_L 300_V 1.176 0.98
236_L 320_Y 1.172 0.98
228_D 231_R 1.168 0.97
303_D 332_R 1.166 0.97
14_R 17_A 1.157 0.97
192_D 196_A 1.155 0.97
290_D 296_I 1.154 0.97
115_I 142_Q 1.151 0.97
245_W 248_V 1.149 0.97
155_L 161_M 1.145 0.97
78_A 152_I 1.145 0.97
140_E 147_L 1.144 0.97
236_L 250_V 1.137 0.97
169_D 172_R 1.124 0.97
85_R 281_Q 1.123 0.97
137_L 141_H 1.116 0.96
61_Q 65_T 1.111 0.96
259_N 276_H 1.101 0.96
202_L 205_P 1.101 0.96
254_T 257_G 1.094 0.96
325_V 328_D 1.086 0.96
238_H 242_K 1.083 0.96
262_A 266_N 1.071 0.95
241_G 270_I 1.069 0.95
3_F 24_P 1.068 0.95
13_L 16_V 1.067 0.95
12_I 57_L 1.061 0.95
38_G 62_H 1.052 0.95
246_Q 315_P 1.047 0.94
40_D 205_P 1.046 0.94
120_M 123_R 1.045 0.94
63_L 77_R 1.045 0.94
287_A 296_I 1.028 0.94
316_H 344_E 1.026 0.94
219_Q 337_G 1.022 0.93
250_V 320_Y 1.02 0.93
16_V 20_G 1.02 0.93
79_L 177_L 1.013 0.93
174_L 181_R 1.007 0.93
34_A 41_L 1.002 0.92
93_E 292_K 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2db3A40.82381000.388Contact Map0.85
3sqwA10.98681000.389Contact Map0.751
4c9bA10.81721000.405Contact Map0.787
2j0sA10.81721000.406Contact Map0.778
3eiqA20.81721000.406Contact Map0.728
3fmpB20.8261000.407Contact Map0.796
3i5xA10.97141000.408Contact Map0.756
2i4iA10.84581000.408Contact Map0.787
1s2mA10.83481000.425Contact Map0.822
3fhtA20.8261000.426Contact Map0.82
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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