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OPENSEQ.org
FLIF - Flagellar M-ring protein
UniProt: P25798 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11347
Length: 552 (534)
Sequences: 728
Seq/Len: 1.36
FLIF
Paralog alert: 0.11 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
258_N 384_N 5.663 1.00
277_E 362_R 3.09 1.00
291_R 359_E 2.912 1.00
131_R 135_G 2.555 1.00
192_V 209_L 2.436 1.00
114_D 131_R 2.416 1.00
72_I 93_L 2.282 0.99
470_K 474_P 2.199 0.99
292_S 357_N 2.197 0.99
111_E 135_G 2.125 0.99
111_E 131_R 2.1 0.98
268_D 375_Q 2.072 0.98
298_S 351_Q 2.065 0.98
409_R 414_F 2.063 0.98
170_S 206_N 2.026 0.98
89_K 92_E 2.021 0.98
404_I 424_V 1.971 0.97
405_E 409_R 1.956 0.97
114_D 135_G 1.927 0.97
469_R 473_R 1.926 0.97
265_A 377_L 1.924 0.97
266_Q 375_Q 1.881 0.96
111_E 139_R 1.858 0.96
456_R 474_P 1.82 0.95
296_N 353_N 1.784 0.94
273_E 366_H 1.757 0.94
388_L 392_K 1.755 0.94
414_F 422_L 1.75 0.94
111_E 114_D 1.727 0.93
246_I 263_V 1.694 0.92
465_W 469_R 1.678 0.92
398_N 402_K 1.677 0.92
48_Y 75_R 1.675 0.91
408_T 422_L 1.673 0.91
238_V 267_L 1.672 0.91
293_R 356_S 1.649 0.91
409_R 422_L 1.647 0.91
402_K 405_E 1.64 0.90
455_G 459_L 1.615 0.89
109_G 135_G 1.612 0.89
86_P 93_L 1.608 0.89
148_K 178_L 1.595 0.89
291_R 360_V 1.587 0.88
471_A 474_P 1.565 0.87
42_W 121_S 1.563 0.87
102_L 193_H 1.548 0.87
238_V 374_V 1.546 0.86
247_E 251_S 1.528 0.86
128_N 131_R 1.523 0.85
469_R 474_P 1.52 0.85
289_A 360_V 1.499 0.84
73_P 86_P 1.498 0.84
147_V 177_L 1.463 0.82
456_R 469_R 1.457 0.82
280_R 361_D 1.454 0.82
275_T 364_I 1.449 0.81
142_E 150_A 1.442 0.81
294_Q 355_T 1.438 0.81
16_R 209_L 1.418 0.79
140_T 238_V 1.416 0.79
410_E 423_N 1.408 0.79
290_L 356_S 1.407 0.78
466_L 470_K 1.406 0.78
112_L 131_R 1.402 0.78
379_V 412_M 1.397 0.78
380_A 407_L 1.377 0.76
109_G 136_E 1.376 0.76
127_V 131_R 1.37 0.76
63_I 102_L 1.366 0.76
136_E 151_R 1.358 0.75
461_L 469_R 1.356 0.75
112_L 135_G 1.353 0.75
247_E 259_I 1.353 0.75
231_Q 269_F 1.35 0.74
162_F 418_R 1.343 0.74
160_S 163_V 1.342 0.74
355_T 424_V 1.334 0.73
295_L 354_E 1.328 0.73
47_D 87_A 1.323 0.72
55_L 63_I 1.316 0.72
177_L 183_L 1.314 0.72
410_E 421_S 1.31 0.71
396_L 400_Q 1.307 0.71
291_R 357_N 1.302 0.71
234_Y 267_L 1.298 0.70
136_E 153_H 1.297 0.70
97_L 103_P 1.291 0.70
121_S 313_L 1.287 0.69
308_G 311_G 1.279 0.69
66_Q 69_Q 1.278 0.68
211_D 215_H 1.274 0.68
193_H 197_S 1.27 0.68
282_N 474_P 1.269 0.68
70_M 93_L 1.267 0.67
186_G 190_A 1.262 0.67
209_L 351_Q 1.262 0.67
48_Y 86_P 1.261 0.67
297_E 352_R 1.255 0.66
121_S 366_H 1.255 0.66
247_E 261_A 1.254 0.66
185_E 422_L 1.252 0.66
312_A 316_Q 1.242 0.65
157_P 169_P 1.24 0.65
85_V 93_L 1.23 0.64
311_G 315_N 1.228 0.64
121_S 190_A 1.219 0.63
313_L 316_Q 1.218 0.63
527_R 544_R 1.212 0.62
292_S 355_T 1.206 0.62
271_S 368_K 1.201 0.61
123_F 131_R 1.2 0.61
111_E 136_E 1.199 0.61
235_A 267_L 1.199 0.61
381_V 408_T 1.196 0.61
142_E 149_G 1.191 0.60
474_P 479_R 1.187 0.60
128_N 135_G 1.187 0.60
18_R 21_P 1.186 0.60
94_R 151_R 1.186 0.60
202_L 389_P 1.185 0.60
38_A 422_L 1.184 0.60
50_T 53_S 1.183 0.60
111_E 153_H 1.177 0.59
80_S 128_N 1.174 0.59
290_L 293_R 1.173 0.59
422_L 534_N 1.172 0.59
112_L 117_K 1.171 0.59
275_T 366_H 1.166 0.58
250_L 254_V 1.165 0.58
246_I 380_A 1.164 0.58
290_L 358_Y 1.164 0.58
138_S 150_A 1.154 0.57
52_F 72_I 1.154 0.57
73_P 89_K 1.154 0.57
137_L 153_H 1.151 0.57
253_I 427_S 1.151 0.57
467_L 470_K 1.15 0.56
311_G 314_S 1.15 0.56
36_M 40_I 1.149 0.56
458_L 461_L 1.146 0.56
454_A 462_L 1.143 0.56
256_N 411_A 1.139 0.55
66_Q 70_M 1.137 0.55
109_G 132_A 1.137 0.55
66_Q 100_Q 1.136 0.55
162_F 420_D 1.134 0.55
30_S 34_A 1.133 0.55
175_V 207_V 1.131 0.55
49_R 87_A 1.126 0.54
122_Q 126_Q 1.122 0.54
192_V 218_T 1.12 0.53
138_S 266_Q 1.119 0.53
52_F 103_P 1.118 0.53
126_Q 158_K 1.118 0.53
77_S 80_S 1.117 0.53
32_A 208_T 1.116 0.53
103_P 193_H 1.107 0.52
110_F 130_Q 1.107 0.52
73_P 543_I 1.105 0.52
94_R 136_E 1.105 0.52
134_E 200_A 1.105 0.52
130_Q 158_K 1.103 0.52
109_G 131_R 1.101 0.52
457_W 468_W 1.1 0.51
252_P 259_I 1.099 0.51
270_A 370_N 1.098 0.51
59_D 140_T 1.093 0.51
63_I 176_N 1.091 0.51
52_F 382_V 1.091 0.51
372_G 546_W 1.09 0.50
68_T 74_Y 1.09 0.50
22_K 32_A 1.089 0.50
108_V 128_N 1.088 0.50
93_L 118_F 1.088 0.50
110_F 135_G 1.081 0.50
129_Y 133_L 1.081 0.50
122_Q 164_R 1.08 0.49
253_I 411_A 1.078 0.49
291_R 355_T 1.078 0.49
490_A 496_V 1.076 0.49
64_V 93_L 1.076 0.49
266_Q 376_R 1.075 0.49
338_Q 342_T 1.075 0.49
311_G 318_A 1.075 0.49
125_E 360_V 1.069 0.48
397_S 400_Q 1.069 0.48
148_K 176_N 1.068 0.48
72_I 83_I 1.068 0.48
68_T 71_N 1.065 0.48
535_D 538_V 1.064 0.48
249_I 407_L 1.063 0.48
50_T 84_E 1.06 0.47
402_K 406_D 1.06 0.47
41_L 379_V 1.06 0.47
188_I 268_D 1.06 0.47
52_F 194_L 1.056 0.47
155_A 200_A 1.054 0.47
407_L 426_N 1.053 0.47
455_G 469_R 1.052 0.47
376_R 418_R 1.051 0.47
208_T 541_L 1.051 0.47
110_F 136_E 1.051 0.47
238_V 242_I 1.048 0.46
283_G 290_L 1.047 0.46
227_L 231_Q 1.046 0.46
113_L 124_S 1.045 0.46
238_V 265_A 1.045 0.46
409_R 412_M 1.043 0.46
532_S 539_V 1.042 0.46
12_E 327_T 1.041 0.46
22_K 309_V 1.038 0.45
96_R 184_D 1.035 0.45
456_R 461_L 1.034 0.45
489_Q 493_R 1.033 0.45
227_L 424_V 1.033 0.45
168_S 203_P 1.032 0.45
310_P 314_S 1.031 0.45
466_L 474_P 1.031 0.45
530_E 534_N 1.03 0.44
472_V 476_L 1.03 0.44
148_K 406_D 1.029 0.44
139_R 359_E 1.028 0.44
34_A 41_L 1.028 0.44
148_K 202_L 1.026 0.44
137_L 212_Q 1.026 0.44
202_L 207_V 1.023 0.44
202_L 540_A 1.021 0.44
332_Q 341_T 1.02 0.43
341_T 344_N 1.02 0.43
121_S 535_D 1.017 0.43
290_L 398_N 1.015 0.43
273_E 361_D 1.014 0.43
51_L 67_L 1.011 0.43
175_V 191_I 1.01 0.42
111_E 119_G 1.009 0.42
377_L 422_L 1.007 0.42
90_V 123_F 1.006 0.42
394_L 397_S 1.006 0.42
125_E 134_E 1.004 0.42
58_Q 102_L 1.004 0.42
196_S 237_D 1.001 0.42
52_F 345_S 1 0.41
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2y9jY240.29891000.651Contact Map0.367
1yj7A40.30251000.658Contact Map0.449
2hfvA10.15467.80.973Contact Map0.474
2rddB10.06753.30.976Contact Map0.25
3hd7A20.146740.90.977Contact Map0.385
2kvcA10.15434.40.978Contact Map0.168
2gqcA10.121427.30.979Contact Map0.358
2lepA10.114126.10.98Contact Map0.5
2epiA40.173920.60.981Contact Map0.369
2wteA20.264519.20.981Contact Map0.238
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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