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OPENSEQ.org
MNMA - tRNA-specific 2-thiouridylase MnmA
UniProt: P25745 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11344
Length: 368 (359)
Sequences: 1758
Seq/Len: 4.90
MNMA
Paralog alert: 0.04 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
305_R 334_D 4.45 1.00
89_E 94_R 4.071 1.00
220_K 270_I 3.604 1.00
223_T 227_D 3.361 1.00
177_Q 180_K 3.355 1.00
23_W 185_L 3.259 1.00
183_E 191_K 3.169 1.00
220_K 228_E 3.111 1.00
7_K 31_Q 3.058 1.00
220_K 231_E 2.967 1.00
308_V 346_A 2.963 1.00
131_R 172_E 2.919 1.00
224_V 281_R 2.822 1.00
27_Q 184_D 2.784 1.00
262_V 274_A 2.746 1.00
180_K 184_D 2.697 1.00
218_P 231_E 2.666 1.00
222_I 270_I 2.488 1.00
57_D 192_K 2.484 1.00
140_R 353_V 2.394 1.00
235_L 271_L 2.321 1.00
292_L 348_F 2.321 1.00
286_G 330_E 2.317 1.00
265_D 268_N 2.293 1.00
290_Q 361_E 2.259 1.00
322_K 330_E 2.255 1.00
36_F 69_L 2.183 1.00
12_M 19_S 2.182 1.00
289_A 346_A 2.159 1.00
159_S 162_Q 2.144 1.00
158_L 162_Q 2.099 1.00
131_R 171_G 2.062 1.00
85_L 101_L 2.053 1.00
180_K 183_E 2.026 1.00
265_D 270_I 2.013 1.00
116_A 165_Q 2.006 1.00
144_G 152_S 1.995 1.00
331_V 348_F 1.983 1.00
156_Y 345_S 1.97 1.00
222_I 268_N 1.966 1.00
8_V 30_Y 1.937 1.00
286_G 332_I 1.91 1.00
263_D 272_V 1.89 1.00
156_Y 357_G 1.881 1.00
287_L 346_A 1.877 1.00
331_V 346_A 1.871 1.00
143_R 309_K 1.869 1.00
174_E 177_Q 1.843 1.00
131_R 352_E 1.84 1.00
303_T 322_K 1.839 1.00
305_R 318_P 1.835 1.00
156_Y 359_I 1.82 1.00
58_A 69_L 1.812 1.00
131_R 145_L 1.809 1.00
317_I 337_V 1.79 1.00
304_M 321_V 1.776 1.00
33_E 70_H 1.769 1.00
10_V 21_S 1.751 1.00
205_K 208_E 1.751 1.00
306_C 319_C 1.75 1.00
9_I 121_A 1.735 1.00
79_W 84_E 1.725 1.00
345_S 359_I 1.721 1.00
207_R 233_Q 1.715 1.00
19_S 189_T 1.714 1.00
19_S 61_V 1.652 0.99
22_A 67_I 1.646 0.99
179_R 194_D 1.629 0.99
25_L 125_A 1.624 0.99
265_D 272_V 1.612 0.99
308_V 319_C 1.612 0.99
12_M 34_G 1.584 0.99
173_L 178_V 1.572 0.99
289_A 358_G 1.561 0.99
221_I 273_V 1.559 0.99
104_K 157_T 1.527 0.99
284_S 340_V 1.527 0.99
64_K 188_V 1.504 0.98
224_V 262_V 1.499 0.98
142_L 352_E 1.499 0.98
305_R 320_T 1.492 0.98
139_S 160_H 1.487 0.98
181_I 185_L 1.462 0.98
133_A 142_L 1.451 0.98
248_I 273_V 1.446 0.98
55_L 69_L 1.442 0.98
197_G 204_R 1.438 0.98
235_L 264_K 1.428 0.98
243_R 258_P 1.417 0.98
88_A 91_K 1.415 0.97
34_G 67_I 1.414 0.97
322_K 332_I 1.41 0.97
6_K 123_Y 1.385 0.97
306_C 355_L 1.383 0.97
309_K 316_D 1.383 0.97
211_G 237_Y 1.372 0.97
84_E 88_A 1.365 0.97
132_R 139_S 1.351 0.96
27_Q 185_L 1.344 0.96
345_S 357_G 1.343 0.96
59_Q 63_D 1.339 0.96
9_I 35_L 1.337 0.96
223_T 229_I 1.332 0.96
287_L 333_F 1.331 0.96
7_K 33_E 1.33 0.96
309_K 312_Y 1.325 0.96
318_P 334_D 1.322 0.96
222_I 228_E 1.316 0.96
203_E 208_E 1.314 0.96
264_K 271_L 1.309 0.95
285_V 332_I 1.308 0.95
297_R 300_F 1.296 0.95
6_K 30_Y 1.285 0.95
81_N 105_E 1.278 0.95
307_T 318_P 1.278 0.95
303_T 323_A 1.27 0.94
143_R 316_D 1.26 0.94
288_I 328_R 1.255 0.94
26_Q 65_L 1.243 0.94
321_V 348_F 1.241 0.94
88_A 92_A 1.228 0.93
100_I 345_S 1.214 0.93
23_W 181_I 1.209 0.92
283_M 338_A 1.205 0.92
109_K 113_E 1.2 0.92
9_I 33_E 1.191 0.92
133_A 140_R 1.188 0.91
306_C 321_V 1.18 0.91
333_F 337_V 1.178 0.91
292_L 358_G 1.171 0.91
23_W 27_Q 1.161 0.90
221_I 229_I 1.142 0.89
8_V 167_L 1.14 0.89
229_I 248_I 1.14 0.89
78_Y 99_D 1.138 0.89
64_K 186_G 1.128 0.88
177_Q 184_D 1.127 0.88
279_H 338_A 1.126 0.88
329_I 348_F 1.125 0.88
222_I 272_V 1.114 0.88
20_V 181_I 1.112 0.87
33_E 121_A 1.112 0.87
76_A 80_D 1.112 0.87
255_T 259_W 1.099 0.87
22_A 34_G 1.097 0.86
329_I 358_G 1.086 0.86
131_R 142_L 1.086 0.86
148_N 176_P 1.085 0.86
218_P 233_Q 1.085 0.86
139_S 164_A 1.084 0.86
189_T 192_K 1.083 0.85
160_H 295_V 1.083 0.85
287_L 331_V 1.076 0.85
257_E 275_Q 1.074 0.85
288_I 330_E 1.073 0.85
221_I 271_L 1.073 0.85
308_V 333_F 1.071 0.85
320_T 334_D 1.069 0.84
228_E 231_E 1.063 0.84
144_G 171_G 1.06 0.84
173_L 181_I 1.053 0.83
142_L 353_V 1.043 0.82
8_V 32_V 1.042 0.82
207_R 210_L 1.042 0.82
154_F 313_R 1.039 0.82
287_L 340_V 1.032 0.81
55_L 71_T 1.025 0.81
111_F 124_I 1.025 0.81
289_A 329_I 1.021 0.81
287_L 308_V 1.015 0.80
40_W 209_F 1.008 0.79
11_G 111_F 1.007 0.79
207_R 237_Y 1.007 0.79
307_T 316_D 1.001 0.79
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2derA211000.241Contact Map0.811
2hmaA10.99461000.267Contact Map0.796
1korA40.84511000.692Contact Map0.406
1k92A10.86141000.739Contact Map0.389
3k32A60.52721000.766Contact Map0.559
2nz2A10.83971000.772Contact Map0.555
1vl2A40.86681000.774Contact Map0.55
3bl5A60.5381000.793Contact Map0.638
3fiuA40.57881000.797Contact Map0.594
3ilvA10.71741000.8Contact Map0.377
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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