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OPENSEQ.org
HFLX - GTPase HflX
UniProt: P25519 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10437
Length: 426 (412)
Sequences: 1679
Seq/Len: 4.08
HFLX
Paralog alert: 0.05 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
63_A 95_L 3.522 1.00
34_V 79_L 3.366 1.00
58_V 66_I 3.262 1.00
130_R 175_Q 2.877 1.00
63_A 92_L 2.851 1.00
70_V 98_C 2.803 1.00
201_S 277_A 2.796 1.00
281_L 315_V 2.731 1.00
70_V 78_V 2.599 1.00
82_H 258_H 2.478 1.00
49_R 53_H 2.464 1.00
284_I 314_L 2.351 1.00
62_K 65_E 2.35 1.00
28_Q 44_V 2.302 1.00
236_L 248_V 2.291 1.00
78_V 96_C 2.186 1.00
31_E 44_V 2.182 1.00
299_N 312_T 2.161 1.00
34_V 39_V 2.123 1.00
147_K 152_L 2.024 1.00
45_I 69_A 1.982 1.00
288_D 294_N 1.981 1.00
32_S 35_S 1.951 1.00
200_V 247_T 1.926 1.00
243_D 358_T 1.891 1.00
369_T 403_Q 1.871 1.00
45_I 66_I 1.857 1.00
36_S 114_R 1.856 1.00
43_Q 69_A 1.853 1.00
122_L 174_I 1.835 1.00
14_V 58_V 1.814 1.00
391_K 403_Q 1.799 1.00
140_W 144_E 1.772 0.99
238_R 246_E 1.77 0.99
67_A 71_K 1.762 0.99
203_V 280_L 1.747 0.99
109_D 128_Q 1.728 0.99
236_L 250_A 1.722 0.99
125_E 129_L 1.716 0.99
78_V 98_C 1.714 0.99
173_Q 177_R 1.704 0.99
367_Q 403_Q 1.701 0.99
182_E 268_K 1.695 0.99
261_H 301_V 1.683 0.99
216_R 350_I 1.656 0.99
36_S 227_Q 1.647 0.99
185_R 272_Q 1.606 0.99
356_A 359_E 1.605 0.99
162_T 166_L 1.604 0.99
171_I 175_Q 1.596 0.99
313_L 356_A 1.593 0.99
179_E 182_E 1.592 0.99
295_I 314_L 1.589 0.99
119_E 185_R 1.583 0.98
118_H 177_R 1.582 0.98
290_R 293_E 1.581 0.98
125_E 170_R 1.58 0.98
64_V 68_E 1.573 0.98
12_V 75_A 1.567 0.98
369_T 401_S 1.561 0.98
140_W 147_K 1.557 0.98
215_N 224_A 1.553 0.98
67_A 95_L 1.549 0.98
138_R 141_T 1.549 0.98
34_V 106_L 1.548 0.98
142_H 159_Q 1.531 0.98
303_E 308_H 1.531 0.98
13_L 30_F 1.527 0.98
212_T 344_A 1.521 0.98
63_A 96_C 1.52 0.98
315_V 353_L 1.515 0.98
286_A 316_M 1.512 0.98
211_S 222_V 1.507 0.98
223_Y 227_Q 1.503 0.98
126_L 171_I 1.497 0.98
138_R 152_L 1.483 0.97
313_L 360_R 1.479 0.97
14_V 80_F 1.471 0.97
380_R 402_L 1.471 0.97
37_A 128_Q 1.463 0.97
380_R 392_E 1.462 0.97
37_A 109_D 1.461 0.97
182_E 272_Q 1.46 0.97
395_E 401_S 1.454 0.97
282_H 298_V 1.446 0.97
353_L 357_L 1.446 0.97
382_R 386_L 1.434 0.97
356_A 360_R 1.43 0.97
284_I 295_I 1.425 0.97
367_Q 405_R 1.415 0.96
218_T 239_I 1.415 0.96
182_E 269_A 1.413 0.96
346_T 349_G 1.413 0.96
319_I 341_W 1.405 0.96
39_V 109_D 1.396 0.96
130_R 262_D 1.396 0.96
92_L 96_C 1.393 0.96
265_A 268_K 1.384 0.96
138_R 147_K 1.383 0.96
368_H 404_V 1.362 0.95
151_G 155_P 1.35 0.95
11_A 39_V 1.338 0.95
371_R 395_E 1.333 0.94
10_Q 40_E 1.329 0.94
216_R 280_L 1.328 0.94
150_I 155_P 1.319 0.94
371_R 398_G 1.314 0.94
316_M 341_W 1.305 0.94
157_E 165_R 1.302 0.93
167_L 171_I 1.3 0.93
193_I 276_Q 1.293 0.93
313_L 357_L 1.282 0.93
14_V 66_I 1.281 0.93
409_V 413_R 1.279 0.93
384_Y 392_E 1.278 0.93
140_W 159_Q 1.275 0.93
122_L 181_V 1.272 0.92
146_Q 152_L 1.266 0.92
58_V 80_F 1.265 0.92
281_L 353_L 1.258 0.92
70_V 96_C 1.258 0.92
140_W 143_L 1.254 0.92
152_L 162_T 1.252 0.92
279_L 357_L 1.249 0.91
117_T 121_K 1.248 0.91
383_F 402_L 1.244 0.91
36_S 256_I 1.237 0.91
57_F 82_H 1.226 0.90
201_S 274_T 1.199 0.89
64_V 67_A 1.184 0.88
112_A 128_Q 1.183 0.88
373_P 376_E 1.181 0.88
114_R 223_Y 1.178 0.88
295_I 316_M 1.175 0.88
184_Q 188_G 1.173 0.88
159_Q 166_L 1.173 0.88
332_D 336_K 1.166 0.87
142_H 166_L 1.164 0.87
370_L 404_V 1.159 0.87
239_I 249_L 1.159 0.87
128_Q 155_P 1.158 0.87
120_G 155_P 1.158 0.87
138_R 142_H 1.156 0.87
222_V 235_T 1.156 0.87
261_H 264_V 1.154 0.87
120_G 234_P 1.146 0.86
282_H 295_I 1.137 0.85
355_Q 359_E 1.137 0.85
43_Q 75_A 1.136 0.85
128_Q 132_L 1.135 0.85
315_V 357_L 1.134 0.85
17_Y 48_S 1.131 0.85
140_W 152_L 1.128 0.85
9_E 99_R 1.128 0.85
64_V 95_L 1.125 0.85
77_V 99_R 1.125 0.85
146_Q 159_Q 1.124 0.85
53_H 60_E 1.12 0.84
58_V 63_A 1.114 0.84
184_Q 187_Q 1.107 0.83
407_P 410_D 1.107 0.83
12_V 69_A 1.106 0.83
301_V 304_E 1.106 0.83
216_R 347_G 1.106 0.83
155_P 298_V 1.104 0.83
52_P 57_F 1.1 0.83
138_R 146_Q 1.099 0.83
182_E 265_A 1.097 0.83
200_V 281_L 1.095 0.82
241_V 247_T 1.092 0.82
371_R 401_S 1.09 0.82
331_R 359_E 1.088 0.82
202_L 214_F 1.087 0.82
42_L 73_T 1.087 0.82
349_G 352_Q 1.084 0.82
68_E 71_K 1.081 0.81
383_F 404_V 1.08 0.81
300_T 304_E 1.077 0.81
296_E 300_T 1.077 0.81
67_A 98_C 1.076 0.81
69_A 73_T 1.072 0.81
287_A 321_M 1.067 0.80
36_S 109_D 1.067 0.80
408_I 412_R 1.062 0.80
9_E 128_Q 1.061 0.80
70_V 75_A 1.061 0.80
242_A 354_F 1.057 0.79
12_V 66_I 1.052 0.79
383_F 387_Q 1.049 0.78
352_Q 355_Q 1.047 0.78
128_Q 160_L 1.046 0.78
150_I 153_R 1.046 0.78
199_T 248_V 1.045 0.78
12_V 43_Q 1.038 0.77
279_L 313_L 1.035 0.77
211_S 224_A 1.035 0.77
140_W 166_L 1.035 0.77
214_F 250_A 1.029 0.77
393_W 401_S 1.027 0.76
288_D 291_V 1.027 0.76
37_A 112_A 1.026 0.76
299_N 314_L 1.024 0.76
39_V 79_L 1.024 0.76
390_E 405_R 1.022 0.76
144_E 152_L 1.022 0.76
176_S 179_E 1.022 0.76
112_A 155_P 1.021 0.76
367_Q 390_E 1.021 0.76
129_L 167_L 1.021 0.76
223_Y 230_A 1.02 0.76
286_A 291_V 1.012 0.75
139_G 142_H 1.003 0.74
178_L 181_V 1.001 0.74
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2qtfA10.82861000.392Contact Map0.733
3geeA10.8851000.767Contact Map0.396
1xzpA10.8851000.774Contact Map0.39
2e87A10.79811000.785Contact Map0.476
3gehA10.88031000.791Contact Map0.496
3j4jA10.65021000.798Contact Map0.375
4a9aA20.66431000.807Contact Map0.621
4kjzA40.65491000.819Contact Map0.599
2qm8A20.6081000.82Contact Map0.619
4b43A10.643299.90.822Contact Map0.599
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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