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OPENSEQ.org
HEMH - Ferrochelatase
UniProt: P23871 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10431
Length: 320 (318)
Sequences: 1523
Seq/Len: 4.79
HEMH
Paralog alert: 0.06 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
170_N 221_A 3.705 1.00
193_Y 215_T 3.465 1.00
90_R 310_E 3.328 1.00
80_S 98_L 3.251 1.00
198_Q 202_D 2.86 1.00
165_N 318_A 2.839 1.00
86_A 306_P 2.832 1.00
241_P 244_M 2.741 1.00
97_A 113_E 2.706 1.00
82_Q 304_A 2.664 1.00
178_A 305_T 2.6 1.00
142_E 145_R 2.52 1.00
161_D 210_R 2.507 1.00
260_G 294_K 2.422 1.00
17_D 104_S 2.42 1.00
188_L 230_M 2.404 1.00
112_D 116_A 2.404 1.00
174_N 307_E 2.394 1.00
285_R 289_L 2.324 1.00
198_Q 241_P 2.235 1.00
29_K 50_R 2.184 1.00
216_R 220_S 2.179 1.00
97_A 117_E 2.133 1.00
219_A 229_V 2.116 1.00
253_M 257_K 2.055 1.00
46_W 50_R 2.054 1.00
176_V 265_M 2.035 1.00
11_A 139_V 2.03 1.00
177_R 221_A 2.028 1.00
20_T 23_A 2.022 1.00
213_T 217_E 2.004 1.00
83_Q 309_I 1.967 1.00
97_A 114_L 1.964 1.00
251_L 262_I 1.941 1.00
201_A 208_P 1.926 1.00
215_T 231_M 1.912 1.00
18_A 23_A 1.906 1.00
124_V 143_L 1.795 1.00
191_L 218_L 1.794 1.00
86_A 309_I 1.792 1.00
55_P 58_S 1.792 1.00
213_T 216_R 1.78 1.00
79_Y 271_A 1.777 1.00
164_D 213_T 1.719 1.00
225_A 228_K 1.696 0.99
199_R 240_E 1.681 0.99
261_H 295_K 1.678 0.99
266_C 280_I 1.674 0.99
167_D 314_N 1.668 0.99
73_G 78_V 1.663 0.99
212_R 233_F 1.653 0.99
209_Q 213_T 1.649 0.99
42_R 46_W 1.638 0.99
110_A 114_L 1.628 0.99
6_T 121_H 1.581 0.99
104_S 109_S 1.578 0.99
249_E 252_K 1.575 0.99
90_R 313_A 1.557 0.99
99_G 110_A 1.541 0.99
196_I 201_A 1.518 0.99
168_Y 172_L 1.517 0.98
95_P 117_E 1.506 0.98
193_Y 231_M 1.498 0.98
121_H 155_G 1.46 0.98
250_T 253_M 1.457 0.98
252_K 256_E 1.452 0.98
77_M 81_R 1.448 0.98
232_T 247_T 1.438 0.98
172_L 218_L 1.42 0.97
119_V 122_I 1.395 0.97
251_L 284_N 1.391 0.97
169_I 221_A 1.382 0.97
114_L 122_I 1.38 0.97
24_V 61_V 1.375 0.97
208_P 212_R 1.365 0.97
191_L 229_V 1.347 0.96
17_D 26_R 1.344 0.96
72_G 82_Q 1.341 0.96
101_S 138_A 1.337 0.96
192_S 234_Q 1.333 0.96
86_A 304_A 1.321 0.96
191_L 265_M 1.32 0.96
11_A 107_L 1.315 0.95
15_T 77_M 1.308 0.95
180_F 263_Q 1.305 0.95
173_A 177_R 1.283 0.95
196_I 200_Y 1.262 0.94
165_N 315_L 1.26 0.94
217_E 220_S 1.258 0.94
163_A 217_E 1.256 0.94
113_E 117_E 1.246 0.93
201_A 209_Q 1.235 0.93
174_N 177_R 1.23 0.93
140_W 158_F 1.219 0.92
63_K 66_A 1.218 0.92
281_A 298_Y 1.209 0.92
45_W 49_L 1.2 0.92
270_A 308_H 1.2 0.92
172_L 302_L 1.199 0.92
236_R 246_Y 1.197 0.92
7_G 110_A 1.19 0.91
191_L 219_A 1.188 0.91
215_T 229_V 1.179 0.91
215_T 219_A 1.161 0.90
286_E 289_L 1.153 0.89
162_Y 270_A 1.145 0.89
286_E 290_G 1.143 0.89
208_P 233_F 1.138 0.89
185_E 228_K 1.129 0.88
309_I 312_M 1.129 0.88
125_L 312_M 1.127 0.88
78_V 82_Q 1.125 0.88
179_S 182_K 1.125 0.88
52_V 56_L 1.119 0.87
9_L 99_G 1.119 0.87
7_G 119_V 1.116 0.87
22_E 26_R 1.112 0.87
230_M 250_T 1.112 0.87
125_L 159_I 1.108 0.87
209_Q 212_R 1.106 0.87
72_G 78_V 1.104 0.86
244_M 249_E 1.098 0.86
272_D 303_N 1.098 0.86
170_N 174_N 1.096 0.86
35_R 42_R 1.096 0.86
29_K 33_S 1.095 0.86
166_H 221_A 1.095 0.86
157_S 319_Y 1.08 0.85
165_N 314_N 1.078 0.85
311_M 315_L 1.077 0.85
164_D 217_E 1.076 0.84
123_V 316_V 1.075 0.84
27_Y 61_V 1.074 0.84
190_L 262_I 1.069 0.84
86_A 89_Q 1.063 0.84
171_A 302_L 1.056 0.83
39_D 199_R 1.055 0.83
70_M 78_V 1.05 0.82
232_T 250_T 1.05 0.82
123_V 157_S 1.047 0.82
109_S 113_E 1.045 0.82
252_K 290_G 1.044 0.82
248_D 287_V 1.042 0.82
284_N 287_V 1.041 0.82
87_L 309_I 1.038 0.81
173_A 221_A 1.038 0.81
87_L 313_A 1.037 0.81
85_Q 88_A 1.033 0.81
263_Q 297_E 1.016 0.80
79_Y 304_A 1.012 0.79
130_Q 211_C 1.012 0.79
110_A 113_E 1.01 0.79
199_R 203_E 1.007 0.79
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1lbqA20.97191000.199Contact Map0.695
3hcnA20.97191000.204Contact Map0.713
2h1vA10.9251000.276Contact Map0.657
2xwpA10.7751000.592Contact Map0.389
2xvyA10.751000.669Contact Map0.378
2jh3A40.753199.60.846Contact Map0.655
3lyhA20.384499.50.848Contact Map0.501
2xwsA10.384499.30.866Contact Map0.749
1tjnA10.456299.30.867Contact Map0.717
4eacA40.309438.10.967Contact Map0.378
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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