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OPENSEQ.org
RECJ - Single-stranded-DNA-specific exonuclease RecJ
UniProt: P21893 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10830
Length: 577 (554)
Sequences: 1601
Seq/Len: 2.89
RECJ
Paralog alert: 0.03 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
400_G 408_M 3.74 1.00
63_I 155_P 3.666 1.00
63_I 72_T 3.552 1.00
93_L 261_R 3.384 1.00
146_V 156_V 3.324 1.00
190_A 232_G 3.221 1.00
86_T 232_G 3.182 1.00
146_V 158_V 3.169 1.00
123_D 148_H 3.142 1.00
441_K 444_L 3.114 1.00
56_G 59_K 3.048 1.00
560_E 565_R 2.989 1.00
512_V 533_I 2.986 1.00
305_A 314_E 2.818 1.00
133_I 154_I 2.781 1.00
94_A 199_M 2.641 1.00
23_P 484_L 2.637 1.00
75_I 125_A 2.59 1.00
192_V 232_G 2.585 1.00
421_G 448_R 2.561 1.00
150_R 172_E 2.546 1.00
55_S 179_L 2.484 1.00
64_L 134_V 2.461 1.00
90_L 192_V 2.445 1.00
56_G 179_L 2.435 1.00
126_H 152_L 2.421 1.00
407_H 410_D 2.391 1.00
424_L 438_E 2.39 1.00
406_L 453_V 2.357 1.00
229_V 247_L 2.312 1.00
502_G 554_Y 2.307 1.00
518_K 531_D 2.299 1.00
72_T 131_Q 2.263 1.00
78_G 88_T 2.249 1.00
74_I 100_C 2.233 1.00
483_Q 565_R 2.223 1.00
95_M 100_C 2.189 1.00
179_L 182_C 2.189 1.00
521_V 552_L 2.165 1.00
56_G 173_A 2.11 1.00
75_I 130_A 2.093 1.00
147_E 170_A 2.086 1.00
396_L 442_F 2.082 1.00
309_C 314_E 2.038 1.00
388_F 396_L 2.037 1.00
93_L 310_D 2.036 1.00
91_S 195_A 2.034 1.00
290_R 323_D 2.028 1.00
227_D 261_R 2 1.00
60_A 175_I 1.986 1.00
468_S 553_A 1.967 1.00
76_V 92_V 1.967 1.00
148_H 152_L 1.961 0.99
447_Q 451_E 1.952 0.99
106_L 113_D 1.942 0.99
72_T 155_P 1.932 0.99
235_A 288_G 1.93 0.99
63_I 172_E 1.91 0.99
63_I 67_A 1.908 0.99
269_E 316_R 1.906 0.99
341_L 380_R 1.904 0.99
555_K 572_D 1.9 0.99
92_V 96_R 1.886 0.99
360_M 387_A 1.879 0.99
444_L 447_Q 1.871 0.99
534_A 537_V 1.867 0.99
155_P 172_E 1.846 0.99
472_L 500_F 1.836 0.99
357_G 449_F 1.834 0.99
400_G 433_A 1.819 0.99
105_Y 304_V 1.819 0.99
182_C 187_K 1.817 0.99
559_N 568_Q 1.815 0.99
327_Q 330_K 1.807 0.99
148_H 151_S 1.799 0.99
323_D 327_Q 1.797 0.99
109_N 112_E 1.794 0.99
466_V 553_A 1.788 0.99
339_E 362_H 1.773 0.99
54_L 175_I 1.749 0.99
437_L 445_F 1.737 0.99
55_S 182_C 1.717 0.98
25_L 481_V 1.713 0.98
147_E 151_S 1.707 0.98
159_T 191_G 1.703 0.98
122_V 133_I 1.698 0.98
197_Y 243_N 1.687 0.98
82_A 231_L 1.684 0.98
551_Q 575_W 1.672 0.98
109_N 299_D 1.667 0.98
119_P 144_A 1.66 0.98
388_F 435_L 1.653 0.98
361_Y 390_P 1.653 0.98
422_M 441_K 1.647 0.98
559_N 566_S 1.647 0.98
53_Q 185_P 1.641 0.98
68_F 100_C 1.638 0.98
107_V 304_V 1.633 0.98
163_L 178_N 1.628 0.97
360_M 377_I 1.628 0.97
56_G 181_D 1.626 0.97
189_L 193_G 1.622 0.97
297_L 332_I 1.617 0.97
388_F 442_F 1.614 0.97
168_L 174_I 1.609 0.97
390_P 396_L 1.608 0.97
36_R 40_E 1.607 0.97
444_L 448_R 1.606 0.97
234_V 283_L 1.606 0.97
552_L 571_I 1.588 0.97
357_G 450_G 1.587 0.97
73_R 102_N 1.567 0.97
499_L 553_A 1.556 0.96
57_V 61_V 1.546 0.96
333_E 376_R 1.545 0.96
438_E 441_K 1.536 0.96
192_V 229_V 1.523 0.96
105_Y 305_A 1.52 0.96
197_Y 200_L 1.515 0.96
171_A 174_I 1.5 0.95
386_I 435_L 1.499 0.95
445_F 449_F 1.497 0.95
88_T 105_Y 1.48 0.95
268_L 278_L 1.461 0.94
396_L 437_L 1.454 0.94
395_T 424_L 1.451 0.94
75_I 104_D 1.45 0.94
68_F 74_I 1.448 0.94
284_G 288_G 1.443 0.94
73_R 104_D 1.434 0.94
377_I 385_V 1.418 0.93
186_S 241_D 1.418 0.93
62_E 205_F 1.405 0.93
66_N 209_Q 1.402 0.93
271_A 290_R 1.401 0.93
118_S 121_V 1.401 0.93
375_S 378_K 1.396 0.92
119_P 148_H 1.378 0.92
294_A 322_L 1.377 0.92
66_N 70_E 1.375 0.92
86_T 192_V 1.373 0.92
91_S 198_L 1.368 0.91
451_E 455_E 1.366 0.91
86_T 190_A 1.365 0.91
390_P 439_E 1.362 0.91
309_C 315_A 1.361 0.91
499_L 572_D 1.354 0.91
503_H 551_Q 1.347 0.91
341_L 345_E 1.347 0.91
266_A 319_A 1.342 0.90
64_L 132_L 1.34 0.90
443_K 446_Q 1.335 0.90
400_G 435_L 1.334 0.90
230_A 251_G 1.331 0.90
143_H 167_T 1.331 0.90
473_S 476_E 1.329 0.90
270_V 323_D 1.325 0.90
67_A 72_T 1.324 0.90
406_L 411_A 1.323 0.90
37_S 40_E 1.32 0.89
205_F 209_Q 1.308 0.89
174_I 179_L 1.295 0.88
184_F 197_Y 1.295 0.88
335_G 339_E 1.293 0.88
234_V 248_T 1.293 0.88
64_L 157_I 1.291 0.88
119_P 123_D 1.29 0.88
482_A 567_L 1.284 0.88
49_L 185_P 1.267 0.87
358_L 381_F 1.263 0.86
100_C 103_I 1.259 0.86
64_L 198_L 1.258 0.86
226_L 229_V 1.258 0.86
367_Q 397_K 1.252 0.86
239_P 494_M 1.243 0.85
49_L 243_N 1.242 0.85
317_V 321_E 1.239 0.85
422_M 448_R 1.235 0.85
334_Q 338_I 1.235 0.85
133_I 149_A 1.235 0.85
61_V 65_Y 1.233 0.85
314_E 317_V 1.231 0.84
63_I 157_I 1.228 0.84
394_G 439_E 1.227 0.84
558_I 565_R 1.225 0.84
52_Q 58_E 1.217 0.84
268_L 287_L 1.207 0.83
60_A 198_L 1.204 0.83
262_P 266_A 1.203 0.83
85_A 291_L 1.2 0.82
471_P 503_H 1.198 0.82
89_A 308_L 1.194 0.82
406_L 457_L 1.191 0.82
521_V 532_G 1.189 0.82
479_M 558_I 1.188 0.81
159_T 194_V 1.188 0.81
92_V 308_L 1.185 0.81
141_S 165_G 1.183 0.81
458_D 461_L 1.183 0.81
484_L 488_A 1.179 0.81
531_D 535_F 1.174 0.80
59_K 173_A 1.173 0.80
55_S 181_D 1.172 0.80
377_I 387_A 1.17 0.80
92_V 103_I 1.17 0.80
370_V 400_G 1.167 0.80
196_F 229_V 1.165 0.80
135_T 159_T 1.163 0.80
85_A 300_M 1.162 0.79
344_C 381_F 1.157 0.79
343_L 362_H 1.147 0.78
193_G 232_G 1.147 0.78
234_V 284_G 1.134 0.77
58_E 62_E 1.122 0.76
51_W 57_V 1.122 0.76
132_L 155_P 1.121 0.76
283_L 288_G 1.119 0.76
113_D 121_V 1.116 0.75
190_A 233_T 1.116 0.75
311_N 314_E 1.115 0.75
532_G 571_I 1.114 0.75
149_A 156_V 1.112 0.75
77_V 121_V 1.111 0.75
321_E 324_A 1.107 0.75
52_Q 201_A 1.105 0.74
140_I 191_G 1.105 0.74
149_A 152_L 1.102 0.74
339_E 365_W 1.101 0.74
123_D 127_A 1.097 0.74
557_D 570_I 1.097 0.74
332_I 369_V 1.092 0.73
396_L 439_E 1.092 0.73
23_P 26_L 1.091 0.73
457_L 461_L 1.088 0.73
54_L 57_V 1.086 0.73
414_R 452_L 1.081 0.72
423_M 435_L 1.081 0.72
386_I 445_F 1.078 0.72
66_N 72_T 1.077 0.72
149_A 154_I 1.077 0.72
186_S 242_A 1.076 0.72
250_Q 253_S 1.074 0.71
96_R 310_D 1.072 0.71
505_R 522_E 1.062 0.70
263_G 306_L 1.062 0.70
150_R 170_A 1.058 0.70
63_I 132_L 1.058 0.70
27_R 31_A 1.053 0.69
486_R 489_G 1.051 0.69
388_F 437_L 1.049 0.69
168_L 171_A 1.043 0.68
534_A 571_I 1.042 0.68
64_L 74_I 1.04 0.68
409_R 426_F 1.039 0.68
143_H 165_G 1.037 0.68
424_L 436_S 1.034 0.67
184_F 243_N 1.034 0.67
501_D 553_A 1.034 0.67
207_R 219_I 1.033 0.67
106_L 121_V 1.028 0.67
398_G 435_L 1.026 0.66
452_L 456_W 1.025 0.66
76_V 136_V 1.022 0.66
231_L 283_L 1.021 0.66
111_F 367_Q 1.02 0.66
43_R 249_W 1.019 0.66
369_V 373_L 1.018 0.66
49_L 197_Y 1.01 0.65
76_V 91_S 1.01 0.65
120_E 123_D 1.01 0.65
435_L 449_F 1.009 0.65
309_C 318_L 1.007 0.64
331_E 335_G 1.005 0.64
144_A 147_E 1 0.63
468_S 501_D 1 0.63
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2zxrA10.90811000.28Contact Map0.728
3dmaA10.55111000.814Contact Map0.46
3devA20.53551000.816Contact Map0.359
1wpnA20.306899.60.913Contact Map0.577
2hawA20.466297.60.956Contact Map0.497
1k20A20.450697.40.958Contact Map0.482
2qb7A20.5286960.964Contact Map0.123
2eb0A20.455893.50.969Contact Map0.287
1id1A20.22778.50.976Contact Map0.398
3ktsA80.246172.80.977Contact Map0.24
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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