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OPENSEQ.org
RSTB - Sensor protein RstB
UniProt: P18392 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11233
Length: 433 (423)
Sequences: 1769
Seq/Len: 4.18
RSTB
Paralog alert: 0.56 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: ATOS BAES BASS CPXA CREC CUSS DCUS DPIB ENVZ GLRK NTRB PHOQ PHOR QSEC RSTB YEDV ZRAS
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
355_E 416_R 4.607 1.00
356_D 360_G 4.564 1.00
370_F 405_V 3.52 1.00
398_A 419_F 3.162 1.00
377_D 380_R 3.107 1.00
230_L 248_L 2.961 1.00
407_C 417_F 2.912 1.00
410_S 413_G 2.851 1.00
398_A 403_G 2.839 1.00
323_V 397_I 2.836 1.00
409_T 415_A 2.79 1.00
366_R 409_T 2.772 1.00
394_V 405_V 2.744 1.00
323_V 419_F 2.563 1.00
397_I 401_M 2.532 1.00
323_V 398_A 2.464 1.00
315_L 320_M 2.421 1.00
173_R 178_H 2.39 1.00
198_F 201_M 2.363 1.00
369_I 407_C 2.362 1.00
365_N 374_V 2.298 1.00
368_H 371_E 2.288 1.00
189_S 192_E 2.232 1.00
229_R 251_D 2.215 1.00
335_C 357_D 2.204 1.00
403_G 421_W 2.19 1.00
395_H 399_L 2.163 1.00
353_I 416_R 2.157 1.00
315_L 401_M 2.134 1.00
227_L 252_I 2.067 1.00
282_L 350_A 2.046 1.00
370_F 395_H 2.02 1.00
356_D 414_G 2.001 1.00
327_L 354_V 1.991 1.00
334_Y 375_R 1.988 1.00
182_R 202_A 1.964 1.00
361_I 369_I 1.954 1.00
278_S 317_M 1.942 1.00
234_L 248_L 1.921 1.00
342_S 355_E 1.897 1.00
398_A 405_V 1.89 1.00
398_A 421_W 1.866 1.00
286_L 341_T 1.842 1.00
182_R 206_N 1.818 1.00
182_R 203_D 1.816 1.00
404_T 420_S 1.785 1.00
408_D 418_S 1.783 1.00
406_N 418_S 1.782 1.00
349_R 420_S 1.78 1.00
279_E 314_A 1.748 0.99
280_P 315_L 1.73 0.99
345_L 423_L 1.73 0.99
362_A 376_L 1.719 0.99
360_G 414_G 1.709 0.99
281_D 310_G 1.709 0.99
281_D 284_L 1.707 0.99
340_E 355_E 1.702 0.99
167_L 198_F 1.668 0.99
408_D 416_R 1.666 0.99
353_I 418_S 1.657 0.99
289_H 325_D 1.651 0.99
351_T 418_S 1.647 0.99
370_F 417_F 1.642 0.99
163_D 190_S 1.612 0.99
231_R 253_S 1.602 0.99
288_T 321_E 1.601 0.99
170_A 198_F 1.593 0.99
340_E 357_D 1.587 0.99
379_S 384_T 1.579 0.99
290_L 341_T 1.578 0.99
279_E 312_Y 1.575 0.99
218_G 372_P 1.571 0.98
351_T 420_S 1.55 0.98
382_R 385_G 1.543 0.98
324_L 352_L 1.537 0.98
346_S 351_T 1.531 0.98
337_S 357_D 1.528 0.98
223_L 255_L 1.51 0.98
365_N 368_H 1.502 0.98
369_I 417_F 1.498 0.98
183_I 199_N 1.484 0.98
403_G 422_P 1.48 0.98
338_T 357_D 1.477 0.97
232_Y 235_E 1.465 0.97
379_S 385_G 1.456 0.97
286_L 343_L 1.436 0.97
365_N 376_L 1.436 0.97
229_R 372_P 1.435 0.97
389_L 393_I 1.426 0.97
254_Q 372_P 1.422 0.97
261_E 322_R 1.405 0.96
363_P 409_T 1.395 0.96
180_N 206_N 1.375 0.96
205_I 208_L 1.361 0.95
357_D 412_L 1.359 0.95
230_L 252_I 1.35 0.95
223_L 259_I 1.348 0.95
380_R 385_G 1.342 0.95
280_P 320_M 1.322 0.94
174_F 202_A 1.319 0.94
362_A 365_N 1.297 0.94
182_R 199_N 1.286 0.93
177_G 202_A 1.283 0.93
167_L 225_T 1.278 0.93
283_P 343_L 1.272 0.93
342_S 353_I 1.268 0.93
229_R 232_Y 1.263 0.92
363_P 410_S 1.252 0.92
399_L 404_T 1.25 0.92
324_L 328_L 1.249 0.92
329_N 333_R 1.24 0.91
151_F 155_I 1.234 0.91
185_F 196_V 1.226 0.91
305_K 340_E 1.216 0.90
231_R 256_E 1.211 0.90
381_D 385_G 1.209 0.90
366_R 415_A 1.204 0.90
375_R 385_G 1.203 0.90
319_L 322_R 1.202 0.90
344_L 353_I 1.2 0.90
261_E 265_Y 1.191 0.89
212_K 222_E 1.19 0.89
286_L 352_L 1.189 0.89
368_H 374_V 1.186 0.89
382_R 386_G 1.182 0.89
356_D 415_A 1.162 0.87
233_R 248_L 1.161 0.87
214_Q 396_S 1.16 0.87
370_F 375_R 1.158 0.87
258_L 261_E 1.135 0.86
352_L 421_W 1.135 0.86
409_T 412_L 1.133 0.86
320_M 401_M 1.129 0.85
166_K 184_H 1.127 0.85
405_V 417_F 1.127 0.85
187_E 192_E 1.125 0.85
322_R 403_G 1.125 0.85
251_D 254_Q 1.122 0.85
355_E 412_L 1.118 0.85
355_E 410_S 1.1 0.83
209_I 212_K 1.095 0.83
215_L 265_Y 1.09 0.83
320_M 421_W 1.085 0.82
359_P 415_A 1.084 0.82
275_L 316_D 1.079 0.82
263_L 266_A 1.076 0.81
172_Q 200_Q 1.075 0.81
327_L 419_F 1.067 0.81
288_T 317_M 1.066 0.81
171_A 175_G 1.06 0.80
232_Y 415_A 1.059 0.80
370_F 407_C 1.056 0.80
215_L 229_R 1.054 0.80
232_Y 236_M 1.053 0.79
328_L 341_T 1.049 0.79
281_D 309_Q 1.044 0.79
352_L 419_F 1.039 0.78
366_R 410_S 1.038 0.78
303_R 338_T 1.038 0.78
350_A 423_L 1.035 0.78
174_F 205_I 1.032 0.78
40_D 227_L 1.031 0.77
78_R 121_A 1.029 0.77
358_G 414_G 1.029 0.77
319_L 323_V 1.028 0.77
344_L 351_T 1.024 0.77
371_E 395_H 1.019 0.76
370_F 394_V 1.017 0.76
277_L 314_A 1.017 0.76
346_S 349_R 1.014 0.76
365_N 378_P 1.009 0.75
120_L 389_L 1.002 0.75
289_H 324_L 1.001 0.74
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4i5sA20.71131000.469Contact Map0.758
2btzA10.70671000.575Contact Map0.558
4ew8A20.58661000.583Contact Map0.746
2q8gA10.72981000.589Contact Map0.536
3jz3A20.48961000.591Contact Map0.656
2e0aA20.71361000.594Contact Map0.585
4e01A10.73671000.595Contact Map0.633
2c2aA10.5521000.6Contact Map0.74
1y8oA10.75521000.606Contact Map0.574
3d36A20.52191000.612Contact Map0.708
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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