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OPENSEQ.org
HYCD - Formate hydrogenlyase subunit 4
UniProt: P16430 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10477
Length: 307 (289)
Sequences: 3354
Seq/Len: 11.61
HYCD
Paralog alert: 0.25 [within 20: 0.05] - ratio of genomes with paralogs
Cluster includes: HYCD HYFC NUOH
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
154_S 159_N 4.75 1.00
233_Q 260_A 3.152 1.00
21_S 42_E 3.138 1.00
28_R 276_N 2.838 1.00
183_A 270_V 2.822 1.00
31_L 276_N 2.644 1.00
179_L 235_F 2.566 1.00
123_T 210_S 2.476 1.00
43_Y 46_I 2.303 1.00
190_M 271_I 2.225 1.00
233_Q 264_L 2.167 1.00
187_F 274_F 2.157 1.00
75_M 227_K 2.151 1.00
240_I 267_G 2.142 1.00
229_L 268_V 2.141 1.00
236_V 260_A 2.094 1.00
232_L 267_G 2.011 1.00
50_L 218_G 2.009 1.00
63_V 113_I 1.984 1.00
242_W 259_I 1.889 1.00
148_A 160_I 1.857 1.00
31_L 273_L 1.84 1.00
183_A 187_F 1.791 1.00
75_M 226_L 1.789 1.00
186_T 231_V 1.746 1.00
159_N 162_D 1.707 1.00
236_V 240_I 1.698 1.00
200_A 204_L 1.682 1.00
17_A 226_L 1.678 1.00
206_E 209_L 1.648 1.00
182_C 235_F 1.64 1.00
67_T 113_I 1.617 1.00
48_K 52_R 1.59 1.00
78_I 230_V 1.589 1.00
99_T 103_L 1.585 1.00
54_S 216_G 1.576 1.00
30_R 35_R 1.561 1.00
147_V 178_A 1.561 1.00
97_L 157_I 1.555 1.00
71_M 110_F 1.488 1.00
23_I 269_L 1.471 1.00
80_T 99_T 1.469 1.00
169_L 179_L 1.464 1.00
71_M 223_G 1.451 1.00
152_A 163_T 1.449 1.00
242_W 252_G 1.437 1.00
81_A 99_T 1.417 1.00
144_G 181_A 1.387 0.99
43_Y 47_I 1.376 0.99
93_Q 99_T 1.369 0.99
97_L 149_A 1.36 0.99
41_Q 45_D 1.339 0.99
17_A 222_W 1.324 0.99
140_M 185_A 1.324 0.99
72_V 223_G 1.317 0.99
57_P 64_F 1.295 0.99
197_L 285_I 1.289 0.99
158_S 162_D 1.284 0.99
115_G 127_A 1.28 0.99
273_L 277_S 1.279 0.99
162_D 165_Y 1.278 0.99
50_L 215_S 1.27 0.99
217_F 221_K 1.262 0.99
183_A 235_F 1.259 0.99
77_T 99_T 1.258 0.99
140_M 290_T 1.237 0.98
126_G 198_A 1.235 0.98
65_R 69_Y 1.234 0.98
70_V 74_V 1.234 0.98
299_L 303_S 1.223 0.98
133_L 286_T 1.219 0.98
283_L 287_P 1.216 0.98
145_L 238_V 1.209 0.98
27_A 269_L 1.208 0.98
140_M 294_F 1.2 0.98
147_V 301_F 1.198 0.98
140_M 297_A 1.197 0.98
267_G 270_V 1.194 0.98
147_V 297_A 1.193 0.98
149_A 155_T 1.191 0.98
197_L 286_T 1.179 0.98
106_I 293_G 1.175 0.98
120_S 123_T 1.174 0.98
161_T 241_P 1.168 0.97
280_R 284_D 1.164 0.97
148_A 238_V 1.161 0.97
182_C 186_T 1.159 0.97
186_T 235_F 1.159 0.97
164_V 241_P 1.153 0.97
178_A 239_F 1.145 0.97
133_L 191_G 1.143 0.97
197_L 281_L 1.137 0.97
156_N 159_N 1.137 0.97
27_A 272_A 1.128 0.97
114_S 117_D 1.126 0.97
175_L 178_A 1.118 0.96
203_E 280_R 1.118 0.96
89_S 92_P 1.115 0.96
152_A 168_P 1.111 0.96
209_L 217_F 1.11 0.96
205_Q 210_S 1.109 0.96
176_V 180_C 1.109 0.96
148_A 175_L 1.107 0.96
152_A 160_I 1.104 0.96
89_S 158_S 1.103 0.96
258_V 262_V 1.099 0.96
130_E 198_A 1.084 0.96
29_A 35_R 1.079 0.95
25_R 34_R 1.078 0.95
262_V 265_V 1.077 0.95
254_L 258_V 1.077 0.95
284_D 287_P 1.076 0.95
22_G 28_R 1.076 0.95
20_L 268_V 1.074 0.95
52_R 292_A 1.073 0.95
268_V 272_A 1.072 0.95
27_A 31_L 1.07 0.95
101_L 142_L 1.07 0.95
200_A 203_E 1.064 0.95
71_M 107_A 1.061 0.95
82_L 233_Q 1.054 0.95
72_V 76_L 1.053 0.95
14_F 18_P 1.052 0.95
247_T 250_A 1.049 0.94
179_L 240_I 1.043 0.94
100_L 230_V 1.043 0.94
77_T 91_L 1.039 0.94
255_L 258_V 1.039 0.94
74_V 103_L 1.028 0.94
167_W 263_K 1.027 0.94
67_T 110_F 1.026 0.94
78_I 227_K 1.025 0.93
182_C 231_V 1.024 0.93
19_L 42_E 1.022 0.93
149_A 152_A 1.021 0.93
108_R 134_G 1.016 0.93
282_R 287_P 1.014 0.93
266_V 270_V 1.003 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4he8H20.97391000.081Contact Map0.707
1q2fA10.097710.30.967Contact Map0.573
2jpkA10.09777.30.969Contact Map0.835
3dacP20.06842.10.976Contact Map0.631
2k8fB10.117320.976Contact Map0.55
3bz1y10.1011.80.977Contact Map0.006
2ll2A10.08471.80.977Contact Map0.049
1pfiA20.08791.60.978Contact Map0
2yevB20.27041.50.978Contact Map0.325
2ks1B10.11071.20.979Contact Map0.043
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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