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OPENSEQ.org
GLGX - Glycogen debranching enzyme
UniProt: P15067 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10381
Length: 657 (651)
Sequences: 3143
Seq/Len: 4.83
GLGX
Paralog alert: 0.81 [within 20: 0.35] - ratio of genomes with paralogs
Cluster includes: AMY2 GLGX MALZ SUCP TREC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
244_F 248_I 5.011 1.00
177_K 243_E 4.19 1.00
190_Q 552_A 4.027 1.00
49_L 59_G 3.962 1.00
177_K 246_D 3.726 1.00
138_W 329_H 3.637 1.00
505_L 654_V 3.507 1.00
323_Y 328_C 3.382 1.00
196_L 251_L 3.287 1.00
245_R 327_T 3.094 1.00
505_L 601_I 3.079 1.00
519_G 547_L 2.894 1.00
250_A 253_K 2.855 1.00
25_L 282_I 2.85 1.00
21_V 64_A 2.769 1.00
186_N 254_A 2.747 1.00
156_A 161_L 2.73 1.00
501_T 652_L 2.668 1.00
264_L 320_C 2.555 1.00
162_T 182_P 2.539 1.00
189_K 254_A 2.533 1.00
20_G 60_Y 2.474 1.00
32_R 48_D 2.455 1.00
570_W 575_G 2.441 1.00
611_I 652_L 2.36 1.00
141_D 329_H 2.331 1.00
37_V 68_L 2.325 1.00
136_Y 249_K 2.27 1.00
556_H 560_R 2.268 1.00
246_D 250_A 2.247 1.00
198_L 247_A 2.24 1.00
185_I 254_A 2.224 1.00
36_C 91_L 2.212 1.00
151_T 195_A 2.154 1.00
597_K 614_T 2.145 1.00
25_L 33_V 2.145 1.00
250_A 254_A 2.124 1.00
65_R 68_L 2.124 1.00
500_L 512_L 2.122 1.00
325_V 366_V 2.104 1.00
33_V 74_V 2.098 1.00
25_L 74_V 2.065 1.00
518_H 538_L 2.064 1.00
139_E 249_K 2.046 1.00
181_H 250_A 2.04 1.00
243_E 246_D 2.019 1.00
505_L 609_I 2.019 1.00
64_A 68_L 2.011 1.00
201_V 244_F 2.004 1.00
23_F 72_Y 1.983 1.00
198_L 201_V 1.974 1.00
42_G 45_H 1.966 1.00
352_P 355_T 1.959 1.00
151_T 367_K 1.952 1.00
561_I 656_Q 1.922 1.00
247_A 251_L 1.905 1.00
236_S 243_E 1.904 1.00
73_R 91_L 1.895 1.00
227_F 320_C 1.89 1.00
93_I 100_I 1.856 1.00
188_L 256_I 1.849 1.00
34_E 73_R 1.831 1.00
314_V 356_A 1.829 1.00
545_S 549_A 1.804 1.00
629_A 655_F 1.803 1.00
140_D 329_H 1.8 1.00
628_H 656_Q 1.788 1.00
269_E 298_N 1.783 1.00
190_Q 548_T 1.777 1.00
402_R 473_Y 1.775 1.00
146_T 195_A 1.774 1.00
203_Q 231_P 1.762 1.00
150_S 565_V 1.749 1.00
21_V 131_V 1.742 1.00
188_L 196_L 1.738 1.00
156_A 188_L 1.719 1.00
244_F 247_A 1.713 1.00
136_Y 246_D 1.705 0.99
153_I 439_V 1.702 0.99
156_A 196_L 1.697 0.99
548_T 552_A 1.695 0.99
259_I 369_I 1.682 0.99
514_A 538_L 1.672 0.99
14_A 21_V 1.666 0.99
448_R 496_I 1.664 0.99
34_E 75_H 1.65 0.99
315_D 319_A 1.646 0.99
244_F 330_V 1.644 0.99
61_L 64_A 1.644 0.99
17_D 60_Y 1.642 0.99
47_Y 61_L 1.64 0.99
136_Y 139_E 1.639 0.99
16_Y 21_V 1.634 0.99
580_L 608_L 1.633 0.99
16_Y 66_P 1.63 0.99
323_Y 327_T 1.629 0.99
76_G 90_K 1.625 0.99
69_R 101_D 1.622 0.99
179_L 247_A 1.616 0.99
21_V 65_R 1.61 0.99
238_E 245_R 1.605 0.99
249_K 253_K 1.604 0.99
564_L 607_F 1.602 0.99
202_A 320_C 1.6 0.99
31_E 75_H 1.599 0.99
161_L 184_M 1.595 0.99
609_I 654_V 1.581 0.99
31_E 77_P 1.58 0.99
267_S 288_Y 1.578 0.99
158_V 198_L 1.565 0.99
172_I 178_A 1.557 0.99
357_I 368_L 1.557 0.99
74_V 282_I 1.554 0.99
512_L 516_D 1.549 0.99
549_A 552_A 1.548 0.99
138_W 141_D 1.547 0.99
398_D 402_R 1.544 0.99
550_F 632_P 1.543 0.99
619_E 644_V 1.538 0.99
601_I 609_I 1.53 0.99
24_T 58_H 1.526 0.99
24_T 56_I 1.526 0.99
396_F 506_S 1.521 0.99
413_A 595_G 1.521 0.99
645_W 655_F 1.521 0.99
100_I 225_A 1.518 0.99
622_L 627_W 1.512 0.98
500_L 551_T 1.512 0.98
136_Y 245_R 1.501 0.98
496_I 519_G 1.495 0.98
177_K 180_G 1.492 0.98
561_I 607_F 1.49 0.98
11_P 22_N 1.489 0.98
610_A 655_F 1.483 0.98
152_I 555_I 1.482 0.98
186_N 190_Q 1.479 0.98
326_E 362_V 1.478 0.98
602_L 608_L 1.47 0.98
501_T 632_P 1.469 0.98
47_Y 59_G 1.469 0.98
99_Q 132_V 1.468 0.98
203_Q 230_H 1.459 0.98
244_F 328_C 1.454 0.98
12_L 24_T 1.452 0.98
158_V 179_L 1.452 0.98
418_F 599_L 1.449 0.98
186_N 189_K 1.444 0.98
598_Q 647_G 1.44 0.98
136_Y 329_H 1.427 0.98
557_L 654_V 1.416 0.97
638_N 643_A 1.408 0.97
34_E 48_D 1.407 0.97
181_H 246_D 1.402 0.97
32_R 75_H 1.399 0.97
97_A 100_I 1.397 0.97
79_Q 82_E 1.396 0.97
610_A 620_I 1.394 0.97
50_P 59_G 1.39 0.97
96_C 288_Y 1.386 0.97
151_T 194_T 1.378 0.97
209_R 213_M 1.376 0.97
96_C 319_A 1.373 0.97
152_I 559_K 1.362 0.97
381_Q 384_N 1.353 0.96
306_L 313_V 1.35 0.96
176_Y 234_A 1.348 0.96
489_V 521_S 1.344 0.96
549_A 553_A 1.342 0.96
64_A 131_V 1.34 0.96
159_K 163_Y 1.335 0.96
504_L 555_I 1.331 0.96
139_E 253_K 1.326 0.96
608_L 622_L 1.325 0.96
500_L 554_L 1.325 0.96
530_C 533_N 1.324 0.96
196_L 247_A 1.323 0.96
158_V 201_V 1.323 0.96
229_L 324_W 1.321 0.96
107_N 125_I 1.315 0.96
100_I 228_A 1.312 0.95
202_A 223_P 1.311 0.95
610_A 647_G 1.307 0.95
505_L 611_I 1.306 0.95
38_F 128_K 1.302 0.95
194_T 256_I 1.302 0.95
493_R 496_I 1.295 0.95
22_N 58_H 1.293 0.95
179_L 251_L 1.293 0.95
312_A 315_D 1.291 0.95
498_A 611_I 1.291 0.95
98_R 323_Y 1.291 0.95
159_K 233_Y 1.284 0.95
280_R 305_T 1.283 0.95
185_I 250_A 1.28 0.95
103_E 207_E 1.274 0.94
33_V 282_I 1.269 0.94
195_A 257_E 1.254 0.94
456_K 470_N 1.253 0.94
28_A 55_D 1.244 0.93
185_I 251_L 1.243 0.93
523_H 535_L 1.242 0.93
201_V 260_L 1.239 0.93
110_L 122_N 1.237 0.93
307_N 310_H 1.236 0.93
98_R 134_D 1.235 0.93
191_L 548_T 1.232 0.93
321_L 333_F 1.23 0.93
308_L 317_A 1.23 0.93
156_A 514_A 1.228 0.93
93_I 129_C 1.228 0.93
245_R 323_Y 1.223 0.93
269_E 303_G 1.222 0.93
14_A 72_Y 1.221 0.93
180_G 246_D 1.221 0.93
180_G 250_A 1.218 0.92
158_V 176_Y 1.216 0.92
557_L 630_I 1.215 0.92
44_E 73_R 1.214 0.92
12_L 56_I 1.211 0.92
67_G 130_V 1.211 0.92
353_L 357_I 1.207 0.92
69_R 130_V 1.202 0.92
71_G 91_L 1.201 0.92
187_Y 548_T 1.199 0.92
84_H 285_R 1.198 0.92
175_T 178_A 1.198 0.92
602_L 606_R 1.197 0.92
617_V 649_A 1.196 0.92
36_C 73_R 1.196 0.92
14_A 23_F 1.192 0.91
501_T 611_I 1.191 0.91
15_H 98_R 1.189 0.91
75_H 89_A 1.188 0.91
66_P 132_V 1.186 0.91
181_H 186_N 1.182 0.91
629_A 634_A 1.178 0.91
383_G 437_N 1.175 0.91
495_S 498_A 1.174 0.91
504_L 512_L 1.173 0.91
188_L 192_G 1.173 0.91
477_H 491_R 1.165 0.90
207_E 302_C 1.158 0.90
251_L 256_I 1.155 0.90
472_N 500_L 1.155 0.90
100_I 129_C 1.152 0.89
581_N 584_A 1.149 0.89
448_R 482_L 1.144 0.89
206_S 216_S 1.14 0.89
512_L 551_T 1.139 0.89
496_I 500_L 1.138 0.89
318_S 357_I 1.136 0.89
229_L 244_F 1.135 0.89
402_R 417_R 1.134 0.88
160_G 218_Y 1.132 0.88
176_Y 229_L 1.13 0.88
156_A 198_L 1.128 0.88
197_E 392_W 1.127 0.88
37_V 69_R 1.122 0.88
68_L 131_V 1.12 0.88
38_F 123_A 1.12 0.88
293_D 343_R 1.116 0.87
421_S 506_S 1.112 0.87
490_E 617_V 1.107 0.87
35_L 61_L 1.107 0.87
359_N 376_A 1.106 0.87
438_L 441_A 1.103 0.87
202_A 260_L 1.101 0.86
474_S 492_R 1.1 0.86
223_P 226_M 1.1 0.86
614_T 618_T 1.096 0.86
249_K 329_H 1.092 0.86
252_H 331_D 1.092 0.86
550_F 630_I 1.091 0.86
367_K 383_G 1.089 0.86
491_R 495_S 1.088 0.86
440_T 516_D 1.088 0.86
399_A 413_A 1.082 0.85
600_Q 610_A 1.081 0.85
97_A 129_C 1.08 0.85
279_L 316_Y 1.079 0.85
291_R 297_H 1.073 0.84
46_R 73_R 1.072 0.84
400_A 502_T 1.071 0.84
382_V 425_F 1.071 0.84
605_D 656_Q 1.068 0.84
406_H 417_R 1.065 0.84
608_L 627_W 1.062 0.84
202_A 330_V 1.061 0.84
193_I 517_E 1.061 0.84
497_H 546_G 1.059 0.83
479_K 485_S 1.056 0.83
69_R 128_K 1.056 0.83
37_V 47_Y 1.048 0.82
140_D 143_P 1.045 0.82
35_L 59_G 1.045 0.82
29_H 86_F 1.038 0.82
500_L 516_D 1.034 0.81
499_L 502_T 1.034 0.81
555_I 559_K 1.028 0.81
647_G 651_G 1.028 0.81
17_D 22_N 1.026 0.81
336_D 371_E 1.026 0.81
291_R 295_D 1.025 0.81
522_Q 528_A 1.022 0.80
489_V 492_R 1.022 0.80
143_P 329_H 1.021 0.80
176_Y 247_A 1.019 0.80
90_K 271_D 1.017 0.80
174_G 182_P 1.015 0.80
260_L 330_V 1.015 0.80
158_V 186_N 1.013 0.79
248_I 330_V 1.011 0.79
169_P 181_H 1.011 0.79
454_N 475_N 1.01 0.79
225_A 228_A 1.009 0.79
234_A 240_A 1.007 0.79
404_W 613_A 1.007 0.79
116_E 573_G 1.006 0.79
299_W 340_V 1.006 0.79
140_D 249_K 1.005 0.79
500_L 547_L 1.005 0.79
298_N 340_V 1.003 0.79
193_I 256_I 1.002 0.78
257_E 367_K 1.001 0.78
27_S 30_A 1 0.78
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2wskA10.99851000.22Contact Map0.677
2vr5A20.99851000.221Contact Map0.769
1bf2A10.97871000.236Contact Map0.665
4aioA10.90111000.339Contact Map0.681
2fhfA10.8951000.347Contact Map0.653
2wanA10.8631000.351Contact Map0.719
2e8yA20.86761000.355Contact Map0.739
2ya1A10.89951000.367Contact Map0.747
3fawA10.89351000.372Contact Map0.675
2ya0A10.8981000.375Contact Map0.757
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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