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OPENSEQ.org
BIOF - 8-amino-7-oxononanoate synthase
UniProt: P12998 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10121
Length: 384 (378)
Sequences: 3230
Seq/Len: 8.54
BIOF
Paralog alert: 0.62 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: BIOF KBL
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
159_A 196_Q 4.468 1.00
63_Q 67_E 4.233 1.00
92_E 281_A 4.071 1.00
301_R 374_D 4.035 1.00
151_A 157_H 3.609 1.00
157_H 161_L 3.424 1.00
341_Q 382_G 3.382 1.00
186_P 189_E 3.36 1.00
159_A 197_H 3.132 1.00
278_A 281_A 3.082 1.00
172_V 194_T 2.91 1.00
174_T 178_F 2.883 1.00
301_R 370_M 2.768 1.00
247_C 251_V 2.723 1.00
60_R 64_Q 2.668 1.00
92_E 284_R 2.607 1.00
305_G 377_L 2.585 1.00
92_E 277_R 2.568 1.00
157_H 160_R 2.526 1.00
105_F 259_A 2.522 1.00
215_Q 221_W 2.462 1.00
155_V 193_V 2.365 1.00
286_D 290_A 2.322 1.00
57_Q 278_A 2.308 1.00
159_A 163_A 2.296 1.00
195_Q 228_E 2.258 1.00
247_C 252_A 2.204 1.00
294_K 298_L 2.199 1.00
127_A 161_L 2.155 1.00
188_A 223_Q 2.152 1.00
205_D 208_G 2.152 1.00
232_V 244_A 2.063 1.00
214_E 221_W 2.04 1.00
44_F 368_H 1.987 1.00
130_R 151_A 1.976 1.00
174_T 203_V 1.974 1.00
95_E 284_R 1.969 1.00
297_A 370_M 1.941 1.00
100_S 249_S 1.921 1.00
128_A 132_S 1.916 1.00
59_I 66_A 1.904 1.00
116_I 173_V 1.873 1.00
189_E 192_Q 1.869 1.00
337_K 340_Q 1.831 1.00
325_V 334_L 1.801 1.00
55_H 282_V 1.801 1.00
369_E 372_D 1.797 1.00
309_L 381_H 1.782 1.00
15_R 20_L 1.76 1.00
305_G 374_D 1.749 1.00
138_E 260_R 1.747 1.00
305_G 308_D 1.742 1.00
304_A 308_D 1.734 1.00
301_R 373_I 1.692 1.00
33_W 40_Q 1.687 1.00
60_R 67_E 1.686 1.00
194_T 201_L 1.678 1.00
120_M 126_I 1.659 1.00
102_A 246_L 1.659 1.00
96_W 217_R 1.658 1.00
172_V 193_V 1.641 1.00
94_A 102_A 1.622 1.00
85_V 277_R 1.61 1.00
126_I 140_A 1.601 1.00
205_D 219_S 1.597 1.00
287_E 293_E 1.594 1.00
93_L 280_L 1.594 1.00
33_W 372_D 1.59 1.00
57_Q 285_S 1.589 1.00
286_D 291_R 1.584 1.00
302_F 364_L 1.58 1.00
59_I 63_Q 1.577 1.00
77_G 82_Y 1.56 1.00
172_V 199_G 1.543 1.00
44_F 376_L 1.525 1.00
82_Y 87_Q 1.507 1.00
12_D 16_A 1.496 1.00
64_Q 68_Q 1.494 1.00
163_A 197_H 1.49 1.00
171_M 202_M 1.488 1.00
249_S 253_D 1.476 1.00
127_A 170_Q 1.476 1.00
151_A 154_D 1.472 1.00
65_G 274_Q 1.464 0.99
136_L 173_V 1.463 0.99
150_F 174_T 1.46 0.99
191_Q 195_Q 1.458 0.99
345_V 360_L 1.448 0.99
329_S 333_Q 1.437 0.99
41_Y 339_R 1.434 0.99
114_A 261_H 1.432 0.99
293_E 297_A 1.429 0.99
140_A 147_L 1.427 0.99
84_V 88_A 1.417 0.99
57_Q 282_V 1.4 0.99
193_V 197_H 1.4 0.99
287_E 290_A 1.388 0.99
134_A 260_R 1.377 0.99
8_N 250_T 1.375 0.99
63_Q 66_A 1.339 0.99
300_T 304_A 1.338 0.99
301_R 305_G 1.335 0.99
155_V 186_P 1.312 0.99
305_G 309_L 1.31 0.99
162_L 193_V 1.309 0.99
152_H 183_D 1.301 0.99
241_S 269_P 1.292 0.98
93_L 234_F 1.289 0.98
88_A 277_R 1.283 0.98
191_Q 201_L 1.279 0.98
234_F 276_L 1.278 0.98
343_C 375_R 1.275 0.98
94_A 246_L 1.272 0.98
115_V 245_V 1.267 0.98
120_M 171_M 1.265 0.98
302_F 306_V 1.259 0.98
370_M 374_D 1.259 0.98
158_L 172_V 1.254 0.98
309_L 377_L 1.252 0.98
181_D 359_R 1.249 0.98
333_Q 337_K 1.238 0.98
110_A 265_S 1.235 0.98
91_E 95_E 1.234 0.98
61_A 65_G 1.233 0.98
15_R 22_R 1.232 0.98
112_N 135_S 1.226 0.98
240_V 276_L 1.225 0.98
161_L 164_S 1.224 0.98
331_A 358_A 1.218 0.97
64_Q 67_E 1.213 0.97
375_R 379_V 1.213 0.97
327_D 330_R 1.206 0.97
150_F 178_F 1.205 0.97
13_A 17_A 1.204 0.97
85_V 273_A 1.198 0.97
73_S 267_S 1.194 0.97
209_T 238_F 1.166 0.96
269_P 272_Q 1.163 0.96
156_T 189_E 1.157 0.96
184_S 212_I 1.156 0.96
299_I 321_Q 1.153 0.96
148_R 161_L 1.15 0.96
156_T 160_R 1.149 0.96
69_F 84_V 1.138 0.96
248_S 251_V 1.137 0.96
220_C 225_V 1.135 0.96
8_N 12_D 1.132 0.96
97_L 246_L 1.124 0.95
365_T 368_H 1.115 0.95
153_N 186_P 1.113 0.95
159_A 193_V 1.109 0.95
290_A 294_K 1.109 0.95
350_P 355_A 1.108 0.95
101_R 249_S 1.107 0.95
56_P 60_R 1.102 0.95
328_N 358_A 1.101 0.95
278_A 282_V 1.099 0.95
166_C 170_Q 1.091 0.94
97_L 209_T 1.081 0.94
132_S 136_L 1.077 0.94
162_L 199_G 1.068 0.93
131_L 359_R 1.064 0.93
11_L 15_R 1.061 0.93
298_L 301_R 1.059 0.93
60_R 63_Q 1.058 0.93
277_R 281_A 1.055 0.93
176_G 219_S 1.054 0.93
343_C 379_V 1.049 0.93
9_A 13_A 1.047 0.93
75_G 79_V 1.045 0.92
332_L 349_R 1.04 0.92
44_F 373_I 1.038 0.92
180_M 348_I 1.035 0.92
176_G 208_G 1.025 0.91
99_Y 246_L 1.018 0.91
87_Q 91_E 1.018 0.91
332_L 347_A 1.016 0.91
10_A 13_A 1.016 0.91
282_V 286_D 1.016 0.91
107_S 110_A 1.014 0.91
294_K 369_E 1.011 0.91
374_D 377_L 1.002 0.90
155_V 189_E 1 0.90
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2w8tA10.99481000.46Contact Map0.669
3kkiA20.97141000.511Contact Map0.754
1bs0A111000.512Contact Map0.652
2bwnA411000.513Contact Map0.797
1fc4A20.99481000.522Contact Map0.818
3tqxA20.99481000.541Contact Map0.812
3a2bA10.99481000.547Contact Map0.783
4iw7A10.95311000.555Contact Map0.684
4e77A10.93751000.65Contact Map0.578
2epjA10.93751000.652Contact Map0.501
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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