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OPENSEQ.org
INSD1 - Transposase InsD for insertion element IS2A
UniProt: P0CF53 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG40004
Length: 301 (277)
Sequences: 3874
Seq/Len: 13.99
INSD1
Paralog alert: 0.70 [within 20: 0.16] - ratio of genomes with paralogs
Cluster includes: INSD1 INSD2 INSD3 INSD4 INSD5 INSD6 INSD8 INSF1 INSF2 INSF3 INSF4 INSF5 INSK INSO1 INSO2 YAGA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
77_R 283_E 3.068 1.00
56_I 71_V 2.746 1.00
126_N 129_W 2.718 1.00
279_R 287_Q 2.667 1.00
177_L 211_F 2.606 1.00
53_L 99_N 2.6 1.00
148_F 237_V 2.588 1.00
130_C 233_A 2.465 1.00
124_E 127_Q 2.452 1.00
280_S 283_E 2.338 1.00
265_E 269_E 2.325 1.00
55_R 78_Q 2.3 1.00
53_L 98_Q 2.27 1.00
156_E 282_R 2.176 1.00
24_S 27_Q 2.144 1.00
251_P 255_T 2.136 1.00
252_D 255_T 2.073 1.00
135_E 143_R 2.049 1.00
161_A 178_G 1.996 1.00
269_E 282_R 1.967 1.00
73_A 278_Y 1.966 1.00
131_S 197_T 1.953 1.00
250_K 259_N 1.943 1.00
123_K 127_Q 1.936 1.00
88_N 91_R 1.903 1.00
148_F 233_A 1.853 1.00
155_R 273_H 1.84 1.00
55_R 58_H 1.816 1.00
49_D 91_R 1.778 1.00
129_W 149_A 1.767 1.00
165_G 168_N 1.761 1.00
129_W 179_A 1.747 1.00
59_V 71_V 1.732 1.00
74_L 272_P 1.725 1.00
79_A 83_G 1.706 1.00
147_T 203_Y 1.705 1.00
72_W 76_R 1.694 1.00
146_V 240_I 1.69 1.00
256_A 260_L 1.671 1.00
238_K 242_R 1.662 1.00
72_W 92_V 1.65 1.00
243_D 271_H 1.626 1.00
279_R 284_Y 1.616 1.00
130_C 152_C 1.611 1.00
161_A 174_D 1.609 1.00
223_A 229_S 1.603 1.00
159_H 175_V 1.598 1.00
209_R 219_P 1.591 1.00
156_E 285_L 1.583 1.00
122_V 128_R 1.58 1.00
240_I 260_L 1.571 1.00
68_Y 93_Y 1.562 1.00
62_E 270_W 1.561 1.00
152_C 232_I 1.561 1.00
13_G 16_L 1.554 1.00
244_Y 259_N 1.552 1.00
244_Y 250_K 1.533 1.00
276_L 280_S 1.519 1.00
56_I 95_I 1.509 1.00
77_R 272_P 1.499 1.00
53_L 95_I 1.492 1.00
235_S 238_K 1.471 1.00
133_G 202_C 1.444 1.00
274_S 278_Y 1.427 1.00
66_Y 70_R 1.415 1.00
26_A 29_H 1.413 1.00
166_G 202_C 1.413 1.00
199_N 224_V 1.409 1.00
74_L 78_Q 1.401 0.99
87_I 92_V 1.381 0.99
150_L 157_A 1.374 0.99
131_S 195_W 1.372 0.99
15_S 25_R 1.361 0.99
131_S 203_Y 1.356 0.99
70_R 243_D 1.347 0.99
55_R 84_M 1.341 0.99
157_A 236_F 1.336 0.99
169_S 173_Q 1.335 0.99
195_W 208_T 1.323 0.99
68_Y 89_A 1.319 0.99
154_D 285_L 1.316 0.99
91_R 94_R 1.303 0.99
48_D 91_R 1.302 0.99
193_V 217_L 1.301 0.99
222_T 229_S 1.298 0.99
52_V 87_I 1.298 0.99
276_L 281_P 1.288 0.99
129_W 193_V 1.288 0.99
212_A 217_L 1.288 0.99
199_N 221_N 1.274 0.99
113_K 116_H 1.272 0.99
138_C 141_G 1.259 0.99
112_S 115_A 1.254 0.99
144_L 253_G 1.252 0.99
59_V 74_L 1.244 0.98
134_F 241_K 1.239 0.98
197_T 219_P 1.237 0.98
212_A 219_P 1.235 0.98
93_Y 97_R 1.234 0.98
268_N 271_H 1.222 0.98
23_V 27_Q 1.222 0.98
136_F 260_L 1.221 0.98
70_R 274_S 1.218 0.98
60_I 101_L 1.217 0.98
147_T 175_V 1.199 0.98
95_I 99_N 1.197 0.98
57_H 99_N 1.195 0.98
25_R 29_H 1.192 0.98
130_C 232_I 1.189 0.98
196_L 229_S 1.188 0.98
58_H 62_E 1.186 0.98
146_V 160_W 1.18 0.98
18_S 28_L 1.179 0.98
14_V 29_H 1.176 0.98
15_S 19_R 1.172 0.98
276_L 284_Y 1.17 0.97
149_A 179_A 1.158 0.97
210_Q 214_M 1.158 0.97
126_N 183_R 1.157 0.97
157_A 264_F 1.156 0.97
163_T 171_T 1.155 0.97
146_V 162_V 1.154 0.97
211_F 215_L 1.15 0.97
222_T 230_N 1.148 0.97
257_A 261_A 1.146 0.97
128_R 152_C 1.13 0.97
180_V 215_L 1.128 0.97
71_V 96_M 1.126 0.97
90_K 94_R 1.121 0.97
196_L 220_K 1.118 0.96
53_L 57_H 1.113 0.96
119_R 128_R 1.112 0.96
235_S 239_T 1.106 0.96
168_N 171_T 1.105 0.96
265_E 270_W 1.104 0.96
74_L 77_R 1.103 0.96
150_L 233_A 1.1 0.96
223_A 226_S 1.097 0.96
72_W 89_A 1.093 0.96
155_R 275_A 1.093 0.96
166_G 201_S 1.089 0.96
153_C 288_R 1.085 0.96
228_E 231_G 1.079 0.95
12_W 17_V 1.074 0.95
149_A 175_V 1.071 0.95
164_T 253_G 1.069 0.95
135_E 145_R 1.058 0.95
76_R 86_A 1.054 0.95
196_L 222_T 1.051 0.94
49_D 94_R 1.043 0.94
280_S 286_R 1.041 0.94
201_S 205_A 1.027 0.94
90_K 93_Y 1.022 0.93
60_I 64_P 1.02 0.93
261_A 265_E 1.016 0.93
273_H 276_L 1.015 0.93
26_A 30_V 1.015 0.93
23_V 31_I 1.01 0.93
255_T 259_N 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4fcyA20.95681000.409Contact Map0.457
3hotA20.9071000.53Contact Map0.638
1bcoA10.60471000.544Contact Map0.508
4fw1A20.58141000.594Contact Map0.659
3av9A20.60471000.598Contact Map0.429
3oymA20.9071000.601Contact Map0.587
3l3uA20.54151000.606Contact Map0.612
1c6vA40.54491000.606Contact Map0.698
3kksA20.5051000.607Contact Map0.57
2x78A30.58141000.614Contact Map0.555
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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