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OPENSEQ.org
SAPB - Peptide transport system permease protein SapB
UniProt: P0AGH3 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG20255
Length: 321 (309)
Sequences: 6826
Seq/Len: 22.09
SAPB
Paralog alert: 0.82 [within 20: 0.29] - ratio of genomes with paralogs
Cluster includes: DDPB DDPC DPPB DPPC GSIC GSID NIKB NIKC OPPB OPPC SAPB SAPC YEJB YEJE
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
37_G 41_W 3.78 1.00
42_N 46_F 3.743 1.00
49_N 53_H 3.484 1.00
20_T 44_W 3.431 1.00
45_V 49_N 3.201 1.00
21_F 44_W 2.97 1.00
219_A 225_R 2.955 1.00
206_T 233_V 2.889 1.00
76_A 265_W 2.808 1.00
215_V 233_V 2.786 1.00
227_T 231_R 2.778 1.00
83_L 187_V 2.613 1.00
242_I 309_A 2.602 1.00
141_L 184_I 2.414 1.00
69_Q 271_W 2.386 1.00
270_R 274_N 2.36 1.00
246_G 302_S 2.291 1.00
19_L 292_G 2.216 1.00
141_L 181_R 2.208 1.00
87_F 191_S 2.204 1.00
217_A 221_R 2.177 1.00
210_Y 233_V 2.057 1.00
243_P 306_G 2.055 1.00
106_W 110_L 2.047 1.00
209_V 214_Y 2.041 1.00
33_A 36_Q 2.018 1.00
65_P 68_E 2 1.00
3_I 7_R 1.978 1.00
224_S 227_T 1.93 1.00
51_L 56_F 1.922 1.00
50_G 55_D 1.92 1.00
102_T 105_K 1.879 1.00
20_T 47_W 1.868 1.00
17_F 21_F 1.865 1.00
90_I 94_P 1.847 1.00
16_L 293_S 1.84 1.00
305_L 308_M 1.833 1.00
62_N 69_Q 1.808 1.00
108_D 203_R 1.758 1.00
91_V 94_P 1.753 1.00
64_Q 68_E 1.748 1.00
209_V 212_Q 1.736 1.00
214_Y 236_N 1.732 1.00
10_L 14_V 1.729 1.00
135_S 143_V 1.722 1.00
95_V 111_I 1.704 1.00
67_A 71_K 1.702 1.00
12_L 296_I 1.696 1.00
66_I 286_A 1.682 1.00
226_F 230_R 1.68 1.00
275_A 283_A 1.648 1.00
125_W 129_L 1.645 1.00
101_I 229_L 1.621 1.00
64_Q 72_E 1.62 1.00
130_L 189_T 1.619 1.00
132_L 137_T 1.613 1.00
133_F 137_T 1.595 1.00
213_N 216_K 1.595 1.00
104_H 108_D 1.595 1.00
209_V 215_V 1.594 1.00
39_S 42_N 1.586 1.00
46_F 49_N 1.572 1.00
191_S 195_T 1.557 1.00
98_I 102_T 1.54 1.00
17_F 44_W 1.533 1.00
141_L 185_L 1.529 1.00
215_V 228_I 1.525 1.00
89_L 245_L 1.525 1.00
69_Q 283_A 1.502 1.00
56_F 286_A 1.496 1.00
272_L 287_G 1.486 1.00
17_F 48_F 1.481 1.00
209_V 237_A 1.471 1.00
221_R 232_H 1.462 1.00
98_I 107_Q 1.456 1.00
118_G 196_T 1.449 1.00
76_A 79_E 1.443 1.00
121_I 126_L 1.441 1.00
271_W 283_A 1.438 1.00
100_G 207_I 1.437 1.00
129_L 133_F 1.432 1.00
133_F 138_L 1.432 1.00
107_Q 111_I 1.426 1.00
91_V 195_T 1.42 1.00
239_P 306_G 1.418 1.00
97_M 206_T 1.417 1.00
92_G 198_V 1.412 1.00
88_A 194_P 1.407 0.99
187_V 191_S 1.405 0.99
100_G 206_T 1.399 0.99
96_G 202_M 1.37 0.99
46_F 50_G 1.369 0.99
12_L 300_V 1.361 0.99
287_G 291_C 1.347 0.99
6_L 10_L 1.337 0.99
111_I 199_I 1.336 0.99
20_T 289_M 1.31 0.99
21_F 25_S 1.288 0.99
16_L 296_I 1.28 0.99
304_I 308_M 1.28 0.99
39_S 46_F 1.276 0.99
126_L 192_V 1.271 0.99
102_T 107_Q 1.268 0.99
96_G 100_G 1.247 0.99
104_H 109_N 1.241 0.98
34_P 38_A 1.24 0.98
127_A 189_T 1.239 0.98
130_L 185_L 1.237 0.98
134_C 138_L 1.231 0.98
180_I 184_I 1.203 0.98
82_I 86_G 1.201 0.98
301_I 305_L 1.193 0.98
72_E 75_P 1.192 0.98
87_F 187_V 1.189 0.98
59_S 280_D 1.187 0.98
298_V 302_S 1.184 0.98
28_Y 281_Y 1.183 0.98
198_V 248_Q 1.18 0.98
85_F 245_L 1.176 0.98
4_F 8_R 1.166 0.97
68_E 71_K 1.166 0.97
35_L 38_A 1.161 0.97
33_A 37_G 1.157 0.97
123_V 259_T 1.156 0.97
256_A 259_T 1.155 0.97
100_G 210_Y 1.154 0.97
37_G 42_N 1.151 0.97
92_G 202_M 1.15 0.97
44_W 48_F 1.147 0.97
79_E 183_M 1.134 0.97
110_L 114_I 1.133 0.97
223_L 228_I 1.124 0.97
45_V 48_F 1.111 0.96
5_T 304_I 1.109 0.96
189_T 193_A 1.106 0.96
9_I 300_V 1.105 0.96
64_Q 69_Q 1.091 0.96
223_L 227_T 1.088 0.96
184_I 188_I 1.088 0.96
250_S 295_V 1.087 0.96
56_F 67_A 1.087 0.96
127_A 262_V 1.086 0.96
4_F 315_K 1.083 0.96
202_M 241_V 1.079 0.95
51_L 289_M 1.072 0.95
4_F 307_A 1.07 0.95
201_L 244_R 1.068 0.95
225_R 228_I 1.06 0.95
99_A 111_I 1.052 0.95
123_V 192_V 1.049 0.94
79_E 83_L 1.044 0.94
2_I 6_L 1.043 0.94
49_N 52_I 1.042 0.94
218_A 223_L 1.032 0.94
218_A 221_R 1.03 0.94
69_Q 72_E 1.025 0.93
49_N 54_W 1.024 0.93
70_L 287_G 1.018 0.93
203_R 207_I 1.016 0.93
79_E 179_A 1.006 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3tuiA40.654299.90.702Contact Map0.743
3rlfF10.859899.80.729Contact Map0.571
2onkC40.822499.80.738Contact Map0.595
3d31C20.831899.80.745Contact Map0.566
3rlfG10.859899.60.779Contact Map0.456
2yevC20.105910.50.966Contact Map0.634
4ev6A50.19636.70.968Contact Map0.79
4humA10.3245.50.97Contact Map0.126
4i0uA100.19634.90.971Contact Map0.413
4lz6A10.32713.50.973Contact Map0.104
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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