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OPENSEQ.org

RPOH - RNA polymerase sigma factor RpoH
UniProt: P0AGB3 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10897
Length: 284 (271)
Sequences: 3519
Seq/Len: 12.99

RPOH
Paralog alert: 0.72 [within 20: 0.07] - ratio of genomes with paralogs
Cluster includes: FLIA RPOH RPOS
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
237_Q 241_R 4.222 1.00
151_Q 156_M 3.549 1.00
50_A 91_R 3.471 1.00
61_V 82_G 3.47 1.00
262_V 267_V 3.322 1.00
155_E 165_S 3.279 1.00
40_K 46_D 3.266 1.00
33_E 52_T 2.994 1.00
51_K 55_L 2.827 1.00
145_R 160_E 2.779 1.00
238_D 259_R 2.756 1.00
36_A 40_K 2.716 1.00
266_R 269_Q 2.637 1.00
260_Y 267_V 2.447 1.00
60_F 102_V 2.153 1.00
236_S 277_K 2.15 1.00
146_L 149_F 2.094 1.00
242_A 259_R 2.08 1.00
243_R 271_E 2.074 1.00
230_Q 237_Q 2.04 1.00
242_A 251_S 1.961 1.00
157_V 168_V 1.955 1.00
239_I 270_L 1.937 1.00
254_Q 258_D 1.877 1.00
39_E 43_Y 1.844 1.00
58_L 83_N 1.828 1.00
130_K 133_R 1.824 1.00
54_I 87_M 1.818 1.00
233_D 277_K 1.814 1.00
239_I 274_A 1.813 1.00
238_D 260_Y 1.801 1.00
209_A 213_E 1.8 1.00
142_T 160_E 1.75 1.00
52_T 56_S 1.732 1.00
231_G 281_A 1.714 1.00
94_N 97_V 1.706 1.00
92_R 104_F 1.699 1.00
155_E 159_R 1.685 1.00
241_R 246_D 1.681 1.00
30_S 33_E 1.649 1.00
265_E 268_R 1.649 1.00
129_T 132_Q 1.632 1.00
238_D 242_A 1.629 1.00
149_F 153_E 1.601 1.00
251_S 259_R 1.58 1.00
35_R 39_E 1.567 1.00
20_I 23_A 1.562 1.00
164_T 167_D 1.548 1.00
64_I 106_V 1.528 1.00
60_F 103_S 1.508 1.00
154_V 168_V 1.495 1.00
65_A 79_I 1.465 1.00
19_Y 23_A 1.459 1.00
31_A 35_R 1.444 1.00
40_K 44_H 1.437 1.00
265_E 269_Q 1.425 1.00
19_Y 83_N 1.414 1.00
23_A 62_V 1.402 0.99
154_V 165_S 1.397 0.99
234_E 237_Q 1.389 0.99
81_E 116_Y 1.38 0.99
154_V 169_R 1.352 0.99
150_N 153_E 1.336 0.99
101_L 105_A 1.328 0.99
146_L 153_E 1.328 0.99
158_A 165_S 1.318 0.99
46_D 49_A 1.297 0.99
142_T 146_L 1.293 0.99
64_I 113_I 1.288 0.99
137_F 141_K 1.28 0.99
266_R 270_L 1.255 0.99
133_R 137_F 1.25 0.99
234_E 238_D 1.22 0.98
158_A 163_V 1.215 0.98
253_L 265_E 1.215 0.98
48_E 51_K 1.212 0.98
253_L 257_A 1.211 0.98
90_V 101_L 1.206 0.98
18_S 22_A 1.206 0.98
159_R 162_G 1.189 0.98
153_E 156_M 1.181 0.98
57_H 102_V 1.176 0.98
142_T 145_R 1.174 0.98
62_V 79_I 1.171 0.98
33_E 36_A 1.165 0.97
47_L 51_K 1.163 0.97
132_Q 138_N 1.144 0.97
100_R 103_S 1.143 0.97
135_L 163_V 1.141 0.97
66_R 75_Q 1.14 0.97
89_A 105_A 1.139 0.97
138_N 142_T 1.132 0.97
167_D 170_E 1.128 0.97
253_L 264_A 1.123 0.97
39_E 96_E 1.116 0.96
138_N 141_K 1.109 0.96
240_I 278_L 1.108 0.96
23_A 26_W 1.106 0.96
253_L 266_R 1.101 0.96
253_L 268_R 1.096 0.96
21_R 26_W 1.093 0.96
253_L 269_Q 1.086 0.96
110_K 114_H 1.086 0.96
196_A 199_L 1.085 0.96
65_A 75_Q 1.075 0.95
63_H 66_R 1.071 0.95
79_I 83_N 1.061 0.95
222_A 226_T 1.061 0.95
53_L 90_V 1.053 0.95
103_S 106_V 1.051 0.94
48_E 52_T 1.042 0.94
235_R 239_I 1.041 0.94
236_S 273_N 1.039 0.94
65_A 113_I 1.037 0.94
26_W 56_S 1.023 0.93
133_R 140_R 1.019 0.93
270_L 274_A 1.017 0.93
158_A 168_V 1.012 0.93
84_I 87_M 1.01 0.93
42_H 96_E 1.004 0.93
132_Q 137_F 1.003 0.93
254_Q 264_A 1.002 0.92
239_I 260_Y 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1l9zH10.96481000.289Contact Map0.067
2a6hF20.96481000.29Contact Map0.535
4kn7X20.96131000.401Contact Map0.582
4ljzF20.96131000.404Contact Map0.587
1l0oC10.84861000.544Contact Map0.808
3ugoA10.60561000.57Contact Map0.822
1rp3A40.80281000.578Contact Map0.745
1sigA10.426199.90.727Contact Map0.701
1or7A20.66999.80.73Contact Map0.633
3vdoA10.654999.80.735Contact Map0.549

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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