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OPENSEQ.org
SPOT - Bifunctional (p)ppGpp synthase/hydrolase SpoT
UniProt: P0AG24 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10966
Length: 702 (699)
Sequences: 1640
Seq/Len: 2.35
SPOT
Paralog alert: 0.47 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: RELA SPOT
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
86_E 91_K 5.113 1.00
427_D 441_Q 3.75 1.00
411_Y 419_H 3.571 1.00
75_I 82_Y 3.288 1.00
61_L 134_L 3.213 1.00
426_V 431_Y 3.183 1.00
25_Q 88_L 3.145 1.00
685_M 697_V 3.118 1.00
269_D 272_T 3.102 1.00
461_V 470_I 3.04 1.00
631_V 688_I 2.973 1.00
232_R 325_E 2.866 1.00
223_R 226_E 2.797 1.00
636_H 642_N 2.717 1.00
407_V 460_F 2.667 1.00
145_R 181_E 2.615 1.00
483_V 525_A 2.586 1.00
23_L 66_L 2.58 1.00
230_P 268_N 2.541 1.00
64_E 92_S 2.474 1.00
268_N 325_E 2.43 1.00
22_R 67_M 2.428 1.00
152_P 155_R 2.409 1.00
490_L 527_I 2.399 1.00
266_I 325_E 2.388 1.00
220_I 280_M 2.353 1.00
417_I 447_T 2.339 1.00
84_D 87_Q 2.217 1.00
437_L 443_V 2.183 1.00
629_I 685_M 2.11 1.00
212_M 312_G 2.088 1.00
252_Q 257_I 1.949 0.99
463_S 466_A 1.932 0.99
517_L 526_E 1.907 0.99
221_E 225_Q 1.903 0.99
392_T 398_V 1.887 0.99
82_Y 98_E 1.882 0.99
657_L 671_I 1.878 0.99
198_I 240_L 1.861 0.99
29_V 80_A 1.856 0.99
11_I 23_L 1.825 0.98
406_P 424_A 1.824 0.98
201_V 254_F 1.802 0.98
75_I 85_M 1.798 0.98
191_Y 194_R 1.773 0.98
56_L 65_T 1.752 0.98
160_R 164_E 1.75 0.98
40_S 150_L 1.732 0.98
152_P 156_R 1.731 0.98
215_K 314_H 1.725 0.98
628_E 672_R 1.712 0.97
435_Q 438_T 1.695 0.97
234_S 328_D 1.688 0.97
77_D 143_N 1.682 0.97
587_S 593_V 1.662 0.97
202_V 240_L 1.658 0.97
147_L 158_I 1.654 0.97
61_L 65_T 1.649 0.96
519_T 522_D 1.647 0.96
436_P 462_V 1.638 0.96
633_M 639_A 1.608 0.96
636_H 639_A 1.584 0.95
147_L 189_A 1.561 0.95
151_R 154_K 1.557 0.95
415_T 419_H 1.555 0.95
222_G 226_E 1.553 0.95
73_D 77_D 1.551 0.95
210_K 214_Q 1.54 0.94
143_N 146_T 1.538 0.94
32_D 35_E 1.53 0.94
574_C 599_C 1.52 0.94
271_D 275_R 1.515 0.94
673_L 684_I 1.506 0.93
448_A 451_A 1.487 0.93
147_L 159_A 1.486 0.93
76_E 143_N 1.468 0.92
213_I 235_G 1.464 0.92
647_I 654_I 1.461 0.92
53_A 66_L 1.46 0.92
73_D 76_E 1.453 0.92
155_R 189_A 1.452 0.92
223_R 283_L 1.448 0.92
238_K 246_K 1.446 0.91
73_D 143_N 1.446 0.91
639_A 691_M 1.444 0.91
267_V 276_V 1.435 0.91
479_R 522_D 1.422 0.91
30_A 46_I 1.421 0.90
77_D 102_K 1.406 0.90
595_H 599_C 1.404 0.90
99_G 132_V 1.404 0.90
425_R 444_E 1.403 0.90
56_L 69_A 1.402 0.90
426_V 435_Q 1.401 0.90
487_R 524_L 1.398 0.90
652_S 680_H 1.397 0.89
63_Y 67_M 1.391 0.89
34_H 73_D 1.391 0.89
643_L 647_I 1.389 0.89
218_S 222_G 1.387 0.89
39_R 45_Y 1.386 0.89
509_Q 513_D 1.384 0.89
80_A 84_D 1.38 0.89
95_E 128_Q 1.38 0.89
102_K 143_N 1.377 0.89
583_I 602_I 1.377 0.89
49_P 143_N 1.374 0.88
39_R 77_D 1.372 0.88
22_R 64_E 1.367 0.88
150_L 154_K 1.367 0.88
96_L 135_I 1.366 0.88
39_R 146_T 1.365 0.88
406_P 426_V 1.362 0.88
573_C 599_C 1.361 0.88
574_C 595_H 1.358 0.88
77_D 146_T 1.358 0.88
48_H 142_H 1.356 0.88
77_D 101_S 1.355 0.87
34_H 143_N 1.348 0.87
76_E 146_T 1.346 0.87
483_V 521_D 1.342 0.87
24_R 28_L 1.341 0.87
290_R 309_S 1.34 0.87
281_H 308_T 1.34 0.87
142_H 146_T 1.335 0.86
28_L 31_R 1.329 0.86
39_R 143_N 1.324 0.86
144_M 182_L 1.323 0.86
56_L 66_L 1.323 0.86
75_I 98_E 1.319 0.86
576_P 599_C 1.314 0.85
584_A 613_M 1.313 0.85
199_K 240_L 1.313 0.85
144_M 147_L 1.309 0.85
67_M 93_V 1.305 0.85
197_V 201_V 1.304 0.85
76_E 101_S 1.302 0.85
29_V 88_L 1.294 0.84
22_R 25_Q 1.293 0.84
594_I 615_V 1.293 0.84
404_A 688_I 1.292 0.84
415_T 571_A 1.29 0.84
205_A 208_N 1.289 0.84
325_E 329_Q 1.289 0.84
55_I 145_R 1.288 0.84
82_Y 95_E 1.287 0.84
48_H 72_H 1.286 0.84
139_D 143_N 1.286 0.84
39_R 42_G 1.284 0.83
34_H 48_H 1.284 0.83
246_K 319_E 1.282 0.83
211_E 215_K 1.28 0.83
34_H 76_E 1.28 0.83
82_Y 86_E 1.278 0.83
34_H 77_D 1.272 0.83
39_R 102_K 1.269 0.82
34_H 37_Q 1.263 0.82
99_G 121_K 1.261 0.82
163_L 241_Y 1.261 0.82
49_P 73_D 1.257 0.82
34_H 45_Y 1.253 0.81
338_H 341_Y 1.252 0.81
212_M 313_P 1.25 0.81
216_I 318_V 1.246 0.81
561_K 616_E 1.245 0.81
66_L 69_A 1.235 0.80
39_R 76_E 1.231 0.80
204_A 316_V 1.231 0.80
194_R 197_V 1.228 0.79
43_E 48_H 1.227 0.79
72_H 76_E 1.227 0.79
206_R 237_E 1.226 0.79
581_P 597_E 1.226 0.79
39_R 142_H 1.226 0.79
277_L 322_I 1.225 0.79
26_A 30_A 1.225 0.79
640_L 657_L 1.221 0.79
150_L 158_I 1.22 0.79
558_L 582_I 1.219 0.79
34_H 72_H 1.213 0.78
304_Q 330_M 1.213 0.78
241_Y 245_C 1.208 0.78
34_H 39_R 1.208 0.78
573_C 595_H 1.202 0.77
180_T 239_H 1.197 0.77
73_D 78_T 1.197 0.77
39_R 49_P 1.195 0.77
48_H 143_N 1.193 0.77
320_V 461_V 1.193 0.77
102_K 139_D 1.191 0.76
268_N 272_T 1.189 0.76
455_A 474_L 1.188 0.76
84_D 88_L 1.186 0.76
86_E 94_A 1.185 0.76
39_R 72_H 1.184 0.76
48_H 73_D 1.183 0.76
276_V 279_Q 1.182 0.76
639_A 642_N 1.181 0.76
101_S 143_N 1.18 0.76
83_Q 86_E 1.178 0.75
478_K 481_D 1.174 0.75
274_Y 281_H 1.174 0.75
73_D 146_T 1.173 0.75
83_Q 87_Q 1.17 0.75
10_L 54_C 1.169 0.75
272_T 275_R 1.166 0.74
34_H 101_S 1.166 0.74
78_T 143_N 1.165 0.74
48_H 77_D 1.164 0.74
219_E 284_Y 1.164 0.74
52_V 139_D 1.163 0.74
647_I 671_I 1.163 0.74
77_D 142_H 1.161 0.74
403_G 436_P 1.155 0.73
39_R 139_D 1.155 0.73
34_H 146_T 1.152 0.73
49_P 102_K 1.151 0.73
587_S 601_N 1.15 0.73
415_T 469_K 1.149 0.73
638_G 653_N 1.146 0.73
635_N 667_Y 1.146 0.73
53_A 69_A 1.146 0.73
49_P 142_H 1.142 0.72
421_C 433_L 1.142 0.72
72_H 139_D 1.141 0.72
21_K 25_Q 1.141 0.72
328_D 332_E 1.14 0.72
73_D 142_H 1.14 0.72
518_A 522_D 1.139 0.72
49_P 77_D 1.139 0.72
39_R 73_D 1.136 0.72
238_K 338_H 1.134 0.71
72_H 143_N 1.133 0.71
75_I 101_S 1.133 0.71
116_A 383_L 1.133 0.71
91_K 95_E 1.132 0.71
412_A 465_K 1.131 0.71
214_Q 218_S 1.13 0.71
576_P 595_H 1.128 0.71
76_E 139_D 1.128 0.71
37_Q 76_E 1.128 0.71
144_M 162_T 1.127 0.71
73_D 101_S 1.127 0.71
42_G 48_H 1.126 0.71
55_I 181_E 1.124 0.70
159_A 186_G 1.123 0.70
34_H 49_P 1.121 0.70
72_H 142_H 1.12 0.70
526_E 532_A 1.117 0.70
42_G 143_N 1.117 0.70
64_E 93_V 1.114 0.69
126_M 130_I 1.111 0.69
631_V 671_I 1.108 0.69
72_H 77_D 1.108 0.69
11_I 16_P 1.107 0.69
224_L 276_V 1.104 0.68
246_K 338_H 1.104 0.68
585_H 611_K 1.103 0.68
546_A 549_P 1.102 0.68
39_R 44_P 1.101 0.68
435_Q 441_Q 1.1 0.68
425_R 430_P 1.099 0.68
73_D 162_T 1.097 0.68
49_P 76_E 1.096 0.68
76_E 142_H 1.094 0.67
246_K 259_D 1.092 0.67
180_T 206_R 1.092 0.67
78_T 146_T 1.092 0.67
404_A 498_R 1.089 0.67
583_I 612_F 1.088 0.67
33_A 84_D 1.088 0.67
246_K 264_R 1.084 0.66
246_K 262_A 1.084 0.66
77_D 139_D 1.083 0.66
39_R 48_H 1.083 0.66
280_M 310_M 1.081 0.66
629_I 671_I 1.081 0.66
71_L 93_V 1.08 0.66
45_Y 72_H 1.079 0.66
76_E 102_K 1.078 0.66
34_H 142_H 1.077 0.66
72_H 146_T 1.077 0.66
647_I 652_S 1.076 0.66
659_T 669_A 1.074 0.65
82_Y 94_A 1.074 0.65
73_D 102_K 1.074 0.65
182_L 186_G 1.073 0.65
216_I 263_F 1.072 0.65
647_I 673_L 1.071 0.65
19_Q 63_Y 1.069 0.65
45_Y 73_D 1.069 0.65
68_A 96_L 1.067 0.65
630_K 670_F 1.067 0.65
10_L 58_E 1.067 0.65
48_H 76_E 1.066 0.64
34_H 102_K 1.065 0.64
573_C 576_P 1.064 0.64
154_K 158_I 1.063 0.64
49_P 72_H 1.062 0.64
359_M 388_I 1.058 0.64
41_S 77_D 1.057 0.64
6_S 10_L 1.057 0.64
264_R 321_Q 1.055 0.63
48_H 146_T 1.052 0.63
274_Y 277_L 1.052 0.63
473_L 477_L 1.052 0.63
274_Y 278_G 1.045 0.62
629_I 673_L 1.045 0.62
45_Y 143_N 1.045 0.62
479_R 483_V 1.041 0.62
647_I 684_I 1.041 0.62
45_Y 142_H 1.041 0.62
75_I 99_G 1.039 0.62
238_K 336_A 1.037 0.61
264_R 342_K 1.037 0.61
101_S 146_T 1.036 0.61
64_E 67_M 1.034 0.61
220_I 320_V 1.034 0.61
8_N 20_I 1.034 0.61
238_K 321_Q 1.033 0.61
11_I 57_A 1.033 0.61
278_G 281_H 1.033 0.61
277_L 281_H 1.03 0.61
180_T 237_E 1.029 0.60
246_K 336_A 1.028 0.60
34_H 78_T 1.027 0.60
56_L 100_V 1.027 0.60
321_Q 338_H 1.026 0.60
165_I 364_E 1.024 0.60
45_Y 48_H 1.021 0.60
139_D 146_T 1.021 0.60
148_G 188_E 1.02 0.59
641_A 644_T 1.02 0.59
102_K 146_T 1.019 0.59
524_L 643_L 1.016 0.59
101_S 139_D 1.016 0.59
563_A 566_V 1.015 0.59
569_T 590_K 1.015 0.59
175_I 179_K 1.015 0.59
196_R 199_K 1.015 0.59
264_R 319_E 1.014 0.59
31_R 35_E 1.013 0.59
319_E 338_H 1.013 0.59
244_Y 248_V 1.013 0.59
16_P 19_Q 1.012 0.59
512_L 520_L 1.011 0.58
637_Q 653_N 1.011 0.58
188_E 199_K 1.008 0.58
14_Y 60_K 1.007 0.58
336_A 341_Y 1.006 0.58
122_M 286_P 1.004 0.58
48_H 52_V 1.004 0.58
49_P 52_V 1.004 0.58
55_I 140_R 1.003 0.58
636_H 667_Y 1 0.57
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1vj7A20.54841000.499Contact Map0.6
3l9dA20.34471000.759Contact Map0.424
3nr1A20.24791000.828Contact Map0.503
2be3A20.30481000.83Contact Map0.501
3nqwA20.2451000.834Contact Map0.464
2ekiA10.119799.80.921Contact Map0.581
3hvzA60.111199.70.934Contact Map1
2kmmA10.10499.30.947Contact Map0.805
1wwtA10.122599.30.948Contact Map0.511
1wxqA10.336299.10.952Contact Map0.718
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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