May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org
YFBS - Uncharacterized transporter YfbS
UniProt: P0AFU2 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG14103
Length: 610 (604)
Sequences: 880
Seq/Len: 1.46
YFBS
Paralog alert: 0.16 [within 20: 0.02] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
69_G 450_M 4.203 1.00
138_M 142_F 3.797 1.00
146_I 189_G 3.351 1.00
47_E 485_K 3.173 1.00
235_I 281_F 2.997 1.00
428_M 446_A 2.891 1.00
431_L 436_E 2.742 1.00
66_I 446_A 2.589 1.00
553_V 604_I 2.523 1.00
490_A 494_K 2.435 1.00
549_P 610_F 2.336 0.99
27_D 462_Y 2.262 0.99
517_A 593_T 2.243 0.99
605_P 610_F 2.219 0.99
605_P 609_P 2.218 0.99
489_V 493_V 2.124 0.99
353_L 384_G 2.003 0.98
342_V 355_V 1.995 0.98
199_M 202_G 1.984 0.98
264_R 285_D 1.973 0.98
197_R 202_G 1.965 0.98
116_A 573_L 1.929 0.97
72_R 460_S 1.917 0.97
9_L 488_G 1.915 0.97
7_W 42_T 1.896 0.97
197_R 201_K 1.832 0.96
66_I 428_M 1.826 0.96
327_A 382_V 1.803 0.95
183_L 187_V 1.803 0.95
545_V 550_F 1.796 0.95
246_L 259_V 1.789 0.95
329_I 382_V 1.775 0.95
586_V 590_V 1.775 0.95
59_L 110_S 1.768 0.95
424_C 449_L 1.763 0.95
447_C 451_G 1.736 0.94
603_M 607_L 1.711 0.93
147_S 154_A 1.706 0.93
242_I 283_A 1.705 0.93
103_A 186_L 1.695 0.93
52_F 58_V 1.693 0.93
161_V 529_A 1.686 0.93
549_P 609_P 1.668 0.92
188_L 599_V 1.66 0.92
44_T 485_K 1.655 0.92
450_M 454_R 1.648 0.92
437_I 445_I 1.64 0.91
521_L 593_T 1.638 0.91
61_A 470_I 1.633 0.91
546_S 549_P 1.624 0.91
63_L 428_M 1.623 0.91
165_L 551_A 1.621 0.91
177_S 548_Y 1.616 0.91
564_T 582_F 1.606 0.90
513_F 553_V 1.603 0.90
15_A 33_V 1.603 0.90
516_S 532_M 1.599 0.90
196_M 199_M 1.588 0.90
49_F 440_P 1.582 0.89
44_T 47_E 1.574 0.89
178_V 555_A 1.572 0.89
504_G 507_M 1.572 0.89
15_A 479_F 1.552 0.88
245_R 248_D 1.55 0.88
106_G 147_S 1.539 0.87
157_P 568_S 1.527 0.87
233_L 289_I 1.506 0.86
6_I 10_S 1.502 0.86
538_A 541_K 1.495 0.85
66_I 425_L 1.484 0.85
180_P 184_V 1.473 0.84
265_W 269_R 1.46 0.83
99_M 143_A 1.457 0.83
112_T 568_S 1.454 0.83
600_C 604_I 1.45 0.83
500_G 511_C 1.441 0.82
50_S 481_V 1.438 0.82
429_V 535_I 1.437 0.82
526_T 568_S 1.433 0.82
54_D 163_S 1.43 0.82
141_S 570_V 1.429 0.82
583_S 586_V 1.429 0.82
179_T 184_V 1.42 0.81
174_S 177_S 1.414 0.81
135_R 138_M 1.401 0.80
447_C 450_M 1.39 0.79
244_Q 248_D 1.376 0.78
424_C 446_A 1.367 0.78
424_C 450_M 1.361 0.77
553_V 557_A 1.35 0.76
156_P 568_S 1.349 0.76
62_A 450_M 1.346 0.76
556_M 604_I 1.345 0.76
196_M 201_K 1.34 0.76
508_M 543_M 1.337 0.75
566_V 610_F 1.334 0.75
503_Y 507_M 1.33 0.75
57_V 113_G 1.326 0.74
19_F 476_M 1.322 0.74
5_L 488_G 1.319 0.74
460_S 486_T 1.317 0.74
99_M 190_I 1.315 0.74
30_A 472_L 1.314 0.74
545_V 610_F 1.313 0.73
63_L 66_I 1.307 0.73
546_S 610_F 1.307 0.73
514_V 544_G 1.304 0.73
54_D 159_L 1.3 0.72
563_M 590_V 1.3 0.72
141_S 574_V 1.298 0.72
184_V 603_M 1.292 0.72
62_A 443_A 1.291 0.72
513_F 557_A 1.285 0.71
63_L 443_A 1.283 0.71
239_S 242_I 1.281 0.71
599_V 603_M 1.279 0.71
150_M 179_T 1.276 0.70
13_A 17_V 1.274 0.70
65_I 453_F 1.269 0.70
341_S 344_E 1.268 0.70
509_L 550_F 1.267 0.70
69_G 446_A 1.263 0.69
144_G 149_M 1.257 0.69
11_L 36_A 1.256 0.69
114_V 568_S 1.256 0.69
188_L 595_I 1.255 0.69
57_V 474_V 1.247 0.68
69_G 83_L 1.242 0.67
98_L 102_V 1.23 0.66
56_N 59_L 1.226 0.66
43_L 478_P 1.223 0.66
563_M 593_T 1.223 0.66
111_S 145_L 1.219 0.65
150_M 186_L 1.212 0.65
514_V 597_M 1.212 0.65
457_D 461_A 1.21 0.64
113_G 478_P 1.209 0.64
112_T 157_P 1.209 0.64
484_Q 490_A 1.207 0.64
31_L 48_V 1.206 0.64
17_V 23_R 1.206 0.64
98_L 122_V 1.203 0.64
605_P 608_F 1.203 0.64
289_I 298_L 1.197 0.63
161_V 533_A 1.196 0.63
113_G 156_P 1.194 0.63
192_Y 592_F 1.193 0.63
25_R 29_V 1.19 0.63
259_V 289_I 1.186 0.62
525_N 559_S 1.183 0.62
7_W 40_S 1.182 0.62
195_V 198_F 1.179 0.61
483_L 488_G 1.177 0.61
173_F 572_T 1.177 0.61
51_G 474_V 1.175 0.61
70_L 80_G 1.175 0.61
536_A 554_V 1.166 0.60
113_G 157_P 1.163 0.60
240_P 248_D 1.159 0.59
521_L 570_V 1.152 0.59
149_M 357_G 1.147 0.58
113_G 568_S 1.146 0.58
20_A 187_V 1.14 0.58
443_A 568_S 1.137 0.57
379_I 402_L 1.136 0.57
179_T 183_L 1.135 0.57
30_A 478_P 1.135 0.57
115_V 570_V 1.129 0.56
110_S 568_S 1.129 0.56
90_S 93_K 1.128 0.56
341_S 512_L 1.125 0.56
181_I 556_M 1.12 0.56
157_P 526_T 1.118 0.55
262_V 279_S 1.117 0.55
38_A 48_V 1.117 0.55
428_M 443_A 1.115 0.55
587_K 598_A 1.113 0.55
184_V 187_V 1.113 0.55
187_V 191_L 1.107 0.54
429_V 433_L 1.106 0.54
56_N 160_V 1.105 0.54
43_L 486_T 1.105 0.54
11_L 33_V 1.104 0.54
462_Y 467_W 1.103 0.54
111_S 520_G 1.101 0.54
104_G 108_F 1.1 0.54
292_S 486_T 1.099 0.53
56_N 110_S 1.098 0.53
19_F 30_A 1.098 0.53
156_P 570_V 1.097 0.53
57_V 165_L 1.096 0.53
325_G 386_W 1.096 0.53
27_D 474_V 1.096 0.53
63_L 112_T 1.095 0.53
110_S 140_L 1.095 0.53
115_V 568_S 1.093 0.53
59_L 568_S 1.093 0.53
237_P 283_A 1.093 0.53
480_A 530_V 1.092 0.53
157_P 555_A 1.089 0.52
119_I 573_L 1.085 0.52
51_G 478_P 1.085 0.52
157_P 570_V 1.084 0.52
149_M 561_A 1.083 0.52
196_M 202_G 1.082 0.52
155_T 553_V 1.081 0.52
513_F 597_M 1.081 0.52
496_L 522_F 1.078 0.51
422_I 426_V 1.076 0.51
56_N 522_F 1.074 0.51
510_G 601_V 1.074 0.51
198_F 201_K 1.074 0.51
427_L 449_L 1.073 0.51
70_L 121_V 1.072 0.51
552_M 608_F 1.072 0.51
162_N 166_L 1.071 0.51
195_V 199_M 1.069 0.50
54_D 110_S 1.067 0.50
483_L 489_V 1.067 0.50
110_S 158_N 1.067 0.50
26_M 524_S 1.065 0.50
513_F 593_T 1.064 0.50
59_L 569_P 1.064 0.50
235_I 283_A 1.063 0.50
527_A 574_V 1.063 0.50
61_A 471_I 1.062 0.50
342_V 358_L 1.062 0.50
521_L 526_T 1.062 0.50
520_G 547_P 1.062 0.50
506_H 605_P 1.061 0.50
63_L 161_V 1.06 0.49
52_F 465_I 1.06 0.49
565_P 584_D 1.059 0.49
494_K 598_A 1.056 0.49
68_D 464_S 1.055 0.49
60_I 526_T 1.055 0.49
69_G 421_A 1.054 0.49
106_G 154_A 1.054 0.49
181_I 475_G 1.051 0.49
110_S 155_T 1.05 0.48
110_S 526_T 1.049 0.48
63_L 568_S 1.049 0.48
520_G 561_A 1.048 0.48
338_I 376_L 1.047 0.48
177_S 604_I 1.045 0.48
327_A 346_G 1.045 0.48
185_V 596_V 1.044 0.48
243_G 257_A 1.043 0.48
11_L 494_K 1.042 0.48
115_V 428_M 1.042 0.48
114_V 141_S 1.041 0.48
581_S 594_I 1.041 0.48
423_F 494_K 1.034 0.47
93_K 153_V 1.033 0.47
111_S 154_A 1.032 0.47
343_R 358_L 1.031 0.46
529_A 574_V 1.029 0.46
140_L 559_S 1.028 0.46
236_R 283_A 1.026 0.46
336_E 357_G 1.025 0.46
513_F 560_A 1.025 0.46
113_G 570_V 1.023 0.46
15_A 29_V 1.021 0.45
118_F 327_A 1.021 0.45
237_P 256_G 1.02 0.45
110_S 569_P 1.02 0.45
141_S 568_S 1.019 0.45
37_F 482_A 1.019 0.45
10_S 187_V 1.018 0.45
11_L 42_T 1.014 0.45
526_T 569_P 1.013 0.45
110_S 529_A 1.011 0.44
111_S 144_G 1.011 0.44
10_S 514_V 1.009 0.44
39_L 480_A 1.006 0.44
237_P 284_R 1.003 0.44
238_G 283_A 1.003 0.44
345_I 572_T 1.002 0.44
251_L 259_V 1 0.43
204_T 544_G 1 0.43
110_S 113_G 1 0.43
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4f35D40.68361000.712Contact Map0.47
4gx0A40.609899.50.91Contact Map0.697
4g65A20.491899.50.911Contact Map0.648
1vctA10.313198.70.937Contact Map0.387
3jxoA20.14198.50.941Contact Map0.734
1lnqA80.460798.50.942Contact Map0.373
2aefA20.285298.10.947Contact Map0.427
3l4bC40.324697.90.95Contact Map0.296
4j91A40.332896.50.96Contact Map0.76
2qi9A20.303330.20.981Contact Map0.215
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
Page generated in 1.4303 seconds.