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OPENSEQ.org
PSPA - Phage shock protein A
UniProt: P0AFM6 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10776
Length: 222 (219)
Sequences: 633
Seq/Len: 2.89
PSPA
Paralog alert: 0.17 [within 20: 0.02] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
79_L 84_E 4.465 1.00
76_E 216_K 3.373 1.00
150_R 181_E 2.993 1.00
72_Q 76_E 2.538 1.00
105_E 109_T 2.509 1.00
142_R 179_E 2.394 1.00
68_E 101_I 2.241 1.00
14_N 27_K 2.013 1.00
31_L 35_E 1.992 1.00
2_G 5_S 1.985 1.00
146_A 182_A 1.944 0.99
71_W 74_K 1.822 0.99
8_A 12_N 1.784 0.99
213_L 217_M 1.779 0.99
76_E 213_L 1.779 0.99
61_I 105_E 1.778 0.99
131_E 135_R 1.768 0.99
58_T 62_E 1.702 0.98
147_N 151_D 1.685 0.98
212_Q 216_K 1.675 0.98
135_R 172_E 1.67 0.98
44_R 126_E 1.661 0.98
208_E 211_A 1.658 0.98
102_K 106_H 1.657 0.98
69_V 73_E 1.643 0.98
98_T 102_K 1.627 0.97
70_E 74_K 1.606 0.97
211_A 215_A 1.554 0.96
47_S 118_M 1.492 0.95
30_R 136_Q 1.474 0.95
207_S 217_M 1.463 0.94
184_S 187_F 1.44 0.94
65_S 105_E 1.429 0.94
20_E 34_Q 1.421 0.93
18_L 22_A 1.393 0.92
22_A 27_K 1.349 0.91
95_Q 99_D 1.338 0.90
210_L 214_K 1.331 0.90
76_E 217_M 1.329 0.90
140_M 144_Q 1.316 0.89
88_R 206_I 1.314 0.89
212_Q 215_A 1.314 0.89
15_I 22_A 1.29 0.88
72_Q 94_K 1.286 0.88
151_D 154_R 1.281 0.87
79_L 217_M 1.275 0.87
109_T 113_D 1.257 0.86
50_A 138_A 1.255 0.86
76_E 80_L 1.251 0.86
88_R 207_S 1.248 0.86
79_L 213_L 1.245 0.85
180_A 197_F 1.197 0.82
102_K 199_E 1.194 0.82
86_L 90_A 1.181 0.81
62_E 66_A 1.176 0.81
89_A 92_I 1.174 0.80
209_Q 212_Q 1.167 0.80
66_A 106_H 1.165 0.80
102_K 105_E 1.158 0.79
15_I 116_A 1.155 0.79
35_E 39_T 1.144 0.78
44_R 79_L 1.133 0.77
65_S 69_V 1.131 0.77
64_A 100_L 1.113 0.75
13_A 17_A 1.111 0.75
195_D 208_E 1.107 0.75
29_V 168_F 1.105 0.74
143_H 171_F 1.099 0.74
149_S 182_A 1.096 0.74
56_Q 60_R 1.093 0.73
72_Q 212_Q 1.075 0.72
29_V 136_Q 1.073 0.71
170_S 173_R 1.072 0.71
11_V 15_I 1.066 0.71
75_A 213_L 1.064 0.70
27_K 52_A 1.063 0.70
10_I 29_V 1.061 0.70
160_K 175_I 1.06 0.70
33_I 129_L 1.053 0.69
192_S 195_D 1.051 0.69
116_A 120_K 1.05 0.69
30_R 140_M 1.048 0.69
207_S 211_A 1.044 0.68
40_L 125_L 1.035 0.67
3_I 15_I 1.029 0.67
28_L 170_S 1.026 0.66
40_L 129_L 1.023 0.66
77_L 81_K 1.022 0.66
129_L 167_R 1.019 0.66
68_E 72_Q 1.017 0.65
40_L 44_R 1.013 0.65
198_A 201_K 1.011 0.65
65_S 101_I 1.011 0.65
90_A 197_F 1.01 0.65
72_Q 216_K 1.008 0.64
83_R 196_Q 1.005 0.64
7_F 19_L 1.005 0.64
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2gd5A40.765890.90.907Contact Map0.472
1i84S20.995590.30.908Contact Map0.053
3frtA20.765889.20.91Contact Map0.402
4abmA40.355980.40.92Contact Map0.515
3ojaB10.729777.40.922Contact Map0.263
3ghgA40.770374.20.924Contact Map0.451
3vkgA20.945966.90.928Contact Map0.253
1c1gA40.941456.40.933Contact Map0.306
1deqA40.666753.80.934Contact Map0.061
2dfsA20.689248.80.936Contact Map0.208
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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