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OPENSEQ.org
NTRB - Nitrogen regulation protein NR(II)
UniProt: P0AFB5 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10387
Length: 349 (339)
Sequences: 17580
Seq/Len: 51.86
NTRB
Paralog alert: 0.83 [within 20: 0.21] - ratio of genomes with paralogs
Cluster includes: ATOS BAES BASS CPXA CREC CUSS DCUS DPIB ENVZ GLRK NTRB PHOQ PHOR QSEC RSTB YEDV ZRAS
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
152_A 171_I 3.272 1.00
18_N 109_A 3.196 1.00
286_E 340_E 2.811 1.00
331_E 342_S 2.71 1.00
197_E 233_H 2.653 1.00
335_W 338_H 2.606 1.00
253_L 288_N 2.579 1.00
320_R 324_D 2.568 1.00
293_P 296_L 2.5 1.00
297_Q 332_F 2.351 1.00
221_I 259_E 2.236 1.00
223_D 263_R 2.236 1.00
282_R 342_S 2.225 1.00
263_R 284_D 2.21 1.00
319_A 330_I 2.209 1.00
24_D 28_A 2.201 1.00
19_S 39_L 2.16 1.00
35_A 38_Q 2.157 1.00
282_R 344_Y 2.15 1.00
284_D 340_E 2.128 1.00
13_L 31_Y 2.123 1.00
284_D 342_S 2.123 1.00
21_L 106_L 2.112 1.00
287_D 291_G 2.112 1.00
149_R 175_A 2.097 1.00
241_V 323_I 2.024 1.00
195_V 235_P 2.021 1.00
156_S 172_I 2.005 1.00
280_A 344_Y 2.004 1.00
233_H 326_H 1.995 1.00
332_F 341_F 1.976 1.00
196_T 232_A 1.946 1.00
156_S 169_K 1.936 1.00
205_R 239_E 1.934 1.00
221_I 261_I 1.914 1.00
292_I 296_L 1.9 1.00
261_I 286_E 1.898 1.00
198_S 201_K 1.882 1.00
115_R 118_S 1.877 1.00
263_R 286_E 1.857 1.00
93_S 109_A 1.853 1.00
200_H 264_T 1.842 1.00
80_E 115_R 1.831 1.00
19_S 108_M 1.829 1.00
241_V 319_A 1.807 1.00
323_I 343_V 1.804 1.00
267_A 282_R 1.802 1.00
34_P 38_Q 1.792 1.00
283_I 343_V 1.783 1.00
334_S 339_T 1.762 1.00
323_I 328_G 1.761 1.00
294_P 297_Q 1.759 1.00
42_Q 85_I 1.755 1.00
50_T 54_E 1.751 1.00
292_I 300_L 1.74 1.00
6_Q 10_G 1.734 1.00
37_Q 44_S 1.704 1.00
301_F 330_I 1.703 1.00
265_R 282_R 1.696 1.00
219_R 259_E 1.691 1.00
259_E 288_N 1.689 1.00
149_R 176_D 1.66 1.00
223_D 261_I 1.636 1.00
241_V 322_L 1.634 1.00
21_L 29_I 1.626 1.00
16_L 20_I 1.606 1.00
253_L 260_I 1.594 1.00
333_T 340_E 1.593 1.00
21_L 36_A 1.592 1.00
266_T 347_I 1.555 1.00
114_Q 118_S 1.549 1.00
14_N 34_P 1.549 1.00
265_R 284_D 1.543 1.00
161_D 164_L 1.542 1.00
333_T 342_S 1.523 1.00
10_G 14_N 1.516 1.00
322_L 326_H 1.515 1.00
297_Q 334_S 1.51 1.00
32_A 36_A 1.503 1.00
151_A 167_Y 1.5 1.00
242_L 283_I 1.5 1.00
93_S 114_Q 1.496 1.00
94_V 108_M 1.489 1.00
258_G 288_N 1.474 1.00
233_H 238_I 1.46 1.00
57_S 60_S 1.446 1.00
210_V 243_L 1.424 1.00
293_P 336_P 1.422 1.00
253_L 258_G 1.419 1.00
156_S 165_L 1.416 1.00
22_L 103_M 1.404 0.99
113_N 116_R 1.403 0.99
329_K 344_Y 1.4 0.99
334_S 338_H 1.395 0.99
153_Q 172_I 1.391 0.99
296_L 299_T 1.376 0.99
32_A 37_Q 1.375 0.99
252_A 306_S 1.37 0.99
148_L 175_A 1.362 0.99
234_D 237_Q 1.359 0.99
34_P 37_Q 1.35 0.99
299_T 305_V 1.341 0.99
152_A 172_I 1.329 0.99
179_R 183_D 1.321 0.99
43_S 46_K 1.321 0.99
324_D 329_K 1.313 0.99
36_A 40_L 1.309 0.99
196_T 230_E 1.305 0.99
148_L 171_I 1.286 0.99
32_A 47_L 1.278 0.99
28_A 51_P 1.276 0.99
229_P 266_T 1.27 0.99
180_N 236_D 1.25 0.99
300_L 332_F 1.25 0.99
205_R 235_P 1.244 0.98
9_A 13_L 1.235 0.98
46_K 85_I 1.234 0.98
91_I 115_R 1.214 0.98
23_I 106_L 1.213 0.98
247_R 251_Q 1.207 0.98
160_P 164_L 1.2 0.98
224_Y 264_T 1.195 0.98
331_E 344_Y 1.187 0.98
128_V 132_D 1.179 0.98
118_S 122_L 1.177 0.98
194_R 232_A 1.173 0.98
42_Q 46_K 1.17 0.97
147_G 174_Q 1.169 0.97
299_T 302_Y 1.169 0.97
237_Q 240_Q 1.163 0.97
151_A 170_V 1.159 0.97
155_L 168_T 1.155 0.97
116_R 120_E 1.155 0.97
200_H 224_Y 1.141 0.97
91_I 112_D 1.139 0.97
296_L 305_V 1.138 0.97
251_Q 308_R 1.134 0.97
22_L 105_L 1.127 0.97
22_L 31_Y 1.126 0.97
237_Q 325_Q 1.123 0.97
37_Q 43_S 1.118 0.96
116_R 119_Q 1.112 0.96
302_Y 305_V 1.104 0.96
82_T 91_I 1.099 0.96
241_V 343_V 1.097 0.96
67_Q 71_E 1.095 0.96
30_H 103_M 1.091 0.96
155_L 164_L 1.088 0.96
170_V 174_Q 1.086 0.96
82_T 89_S 1.083 0.96
210_V 246_V 1.081 0.95
235_P 239_E 1.078 0.95
172_I 176_D 1.074 0.95
51_P 54_E 1.074 0.95
90_H 110_P 1.074 0.95
246_V 260_I 1.073 0.95
78_D 93_S 1.07 0.95
174_Q 177_R 1.068 0.95
292_I 332_F 1.066 0.95
141_I 178_L 1.063 0.95
292_I 297_Q 1.06 0.95
152_A 175_A 1.057 0.95
23_I 29_I 1.057 0.95
96_A 104_I 1.054 0.95
281_A 347_I 1.054 0.95
198_S 230_E 1.047 0.94
242_L 246_V 1.043 0.94
147_G 151_A 1.033 0.94
290_P 309_E 1.031 0.94
254_G 257_G 1.029 0.94
206_V 239_E 1.026 0.94
24_D 30_H 1.025 0.93
158_A 164_L 1.022 0.93
214_L 250_L 1.02 0.93
200_H 204_E 1.015 0.93
204_E 222_R 1.01 0.93
197_E 202_V 1.01 0.93
9_A 12_I 1.008 0.93
199_I 283_I 1.006 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4gczA20.97421000.18Contact Map0.81
3a0rA10.95421000.192Contact Map0.619
4i5sA20.92551000.247Contact Map0.84
4ew8A20.69631000.456Contact Map0.831
3d36A20.63041000.461Contact Map0.855
2c2aA10.65331000.462Contact Map0.784
3jz3A20.59031000.47Contact Map0.71
4e01A10.9371000.475Contact Map0.664
2btzA10.83951000.484Contact Map0.6
2q8gA10.85671000.492Contact Map0.609
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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