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OPENSEQ.org
GYRA - DNA gyrase subunit A
UniProt: P0AES4 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10423
Length: 875 (805)
Sequences: 2200
Seq/Len: 2.73
GYRA
Paralog alert: 0.64 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: GYRA PARC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
74_I 85_V 6.804 1.00
374_R 489_L 5.26 1.00
199_D 503_R 4.811 1.00
188_T 514_G 4.81 1.00
698_S 724_I 4.6 1.00
196_A 203_I 4.462 1.00
192_N 510_R 4.2 1.00
193_G 212_I 3.776 1.00
197_Y 351_I 3.56 1.00
112_I 301_M 3.423 1.00
490_R 494_S 3.363 1.00
63_Y 104_D 3.311 1.00
196_A 211_H 3.293 1.00
141_E 371_R 3.202 1.00
68_R 76_K 3.141 1.00
487_E 497_R 3.138 1.00
246_A 321_Y 3.109 1.00
197_Y 203_I 3.034 1.00
490_R 497_R 3.021 1.00
385_V 450_L 2.994 1.00
487_E 490_R 2.981 1.00
163_I 168_V 2.824 1.00
651_A 681_E 2.813 1.00
59_W 132_H 2.78 1.00
100_Y 515_D 2.777 1.00
371_R 375_D 2.755 1.00
69_V 125_I 2.737 1.00
392_P 440_E 2.678 1.00
188_T 192_N 2.672 1.00
140_K 369_E 2.583 1.00
205_I 346_N 2.551 1.00
182_I 334_M 2.536 1.00
380_L 474_L 2.535 1.00
146_V 157_D 2.518 1.00
677_V 719_T 2.516 1.00
699_A 730_D 2.515 1.00
144_D 364_R 2.508 1.00
704_V 768_T 2.501 1.00
722_R 725_R 2.491 1.00
338_H 345_M 2.491 1.00
198_I 499_M 2.485 1.00
649_A 696_L 2.457 1.00
188_T 510_R 2.454 1.00
246_A 259_I 2.454 1.00
699_A 731_K 2.451 1.00
66_S 89_I 2.424 1.00
396_L 409_A 2.407 1.00
188_T 516_K 2.395 1.00
290_S 308_K 2.394 1.00
714_M 718_T 2.391 1.00
133_E 513_F 2.374 1.00
204_S 207_G 2.37 1.00
208_L 347_L 2.367 1.00
456_Q 460_D 2.348 1.00
372_K 375_D 2.341 1.00
191_I 506_L 2.333 1.00
704_V 764_K 2.326 1.00
508_L 512_Q 2.301 1.00
147_D 151_G 2.292 1.00
761_Y 773_S 2.287 1.00
545_H 577_F 2.283 1.00
214_G 229_I 2.272 1.00
363_T 367_I 2.271 1.00
727_G 769_K 2.255 1.00
693_E 705_R 2.254 1.00
206_E 234_R 2.233 1.00
257_E 313_G 2.219 1.00
48_V 88_T 2.201 1.00
208_L 348_K 2.194 1.00
359_R 495_A 2.194 1.00
690_G 708_E 2.187 1.00
83_S 87_D 2.179 1.00
84_A 88_T 2.144 1.00
595_S 681_E 2.117 1.00
64_K 68_R 2.1 1.00
190_V 212_I 2.098 1.00
498_L 501_V 2.093 1.00
651_A 680_D 2.091 1.00
37_V 353_A 2.09 1.00
539_V 604_V 2.089 1.00
168_V 190_V 2.084 1.00
244_A 261_V 2.076 1.00
245_R 520_E 2.076 1.00
748_T 774_I 2.067 1.00
200_D 207_G 2.062 1.00
196_A 200_D 2.052 1.00
734_S 786_A 2.05 1.00
395_E 399_H 2.041 1.00
500_E 504_E 2.039 1.00
724_I 732_V 2.038 1.00
376_R 477_E 2.03 1.00
484_Q 488_L 2.022 1.00
197_Y 355_V 2.02 1.00
147_D 154_K 2.02 1.00
369_E 488_L 2.018 1.00
104_D 126_R 2.013 1.00
203_I 211_H 2.008 1.00
487_E 491_I 2.002 1.00
205_I 348_K 1.987 0.99
407_K 456_Q 1.975 0.99
246_A 317_L 1.969 0.99
261_V 305_I 1.966 0.99
688_T 708_E 1.942 0.99
655_V 723_G 1.937 0.99
203_I 207_G 1.937 0.99
362_V 502_I 1.932 0.99
89_I 125_I 1.92 0.99
489_L 493_G 1.913 0.99
696_L 723_G 1.907 0.99
584_A 588_D 1.905 0.99
366_T 488_L 1.893 0.99
163_I 509_V 1.888 0.99
647_F 655_V 1.887 0.99
195_L 507_E 1.877 0.99
247_E 262_H 1.864 0.99
596_R 628_D 1.855 0.99
823_I 826_R 1.842 0.99
764_K 768_T 1.842 0.99
241_Y 327_Q 1.837 0.99
800_D 831_E 1.816 0.99
129_K 512_Q 1.811 0.99
230_E 234_R 1.811 0.99
205_I 234_R 1.808 0.99
749_Q 832_N 1.794 0.99
689_S 692_D 1.794 0.99
272_R 275_E 1.792 0.99
68_R 75_G 1.781 0.99
375_D 453_Q 1.774 0.99
808_R 811_E 1.774 0.99
497_R 500_E 1.77 0.99
472_E 476_D 1.759 0.98
762_P 771_V 1.743 0.98
494_S 497_R 1.726 0.98
250_V 257_E 1.723 0.98
68_R 72_D 1.723 0.98
256_R 290_S 1.721 0.98
480_E 484_Q 1.706 0.98
385_V 389_N 1.706 0.98
194_C 355_V 1.705 0.98
470_E 473_K 1.704 0.98
348_K 352_A 1.7 0.98
407_K 452_E 1.693 0.98
697_F 786_A 1.678 0.98
773_S 783_V 1.667 0.98
182_I 350_I 1.666 0.98
338_H 353_A 1.659 0.98
654_T 722_R 1.654 0.97
536_Q 585_N 1.65 0.97
359_R 499_M 1.649 0.97
195_L 503_R 1.633 0.97
37_V 350_I 1.632 0.97
399_H 409_A 1.632 0.97
594_S 631_I 1.62 0.97
133_E 136_A 1.619 0.97
68_R 153_E 1.617 0.97
90_V 117_A 1.611 0.97
710_S 765_S 1.606 0.97
752_Y 823_I 1.595 0.97
764_K 771_V 1.594 0.97
68_R 120_M 1.594 0.97
491_I 501_V 1.591 0.97
403_P 464_Q 1.578 0.96
343_K 349_D 1.576 0.96
264_I 303_I 1.557 0.96
346_N 349_D 1.546 0.96
251_D 258_T 1.545 0.96
354_F 358_R 1.541 0.96
611_T 614_A 1.54 0.96
273_L 303_I 1.539 0.96
51_A 74_I 1.525 0.95
585_N 588_D 1.525 0.95
363_T 492_L 1.499 0.95
595_S 629_E 1.488 0.95
200_D 203_I 1.478 0.94
51_A 72_D 1.478 0.94
393_I 410_L 1.471 0.94
649_A 735_L 1.467 0.94
377_A 478_Y 1.467 0.94
334_M 350_I 1.46 0.94
402_T 405_E 1.456 0.94
537_E 586_T 1.455 0.94
746_T 773_S 1.446 0.93
221_A 244_A 1.444 0.93
647_F 694_V 1.443 0.93
72_D 149_Y 1.436 0.93
476_D 480_E 1.435 0.93
593_F 685_V 1.435 0.93
601_S 666_L 1.43 0.93
241_Y 329_S 1.429 0.93
531_E 603_K 1.425 0.93
807_T 824_L 1.421 0.93
353_A 356_R 1.42 0.93
206_E 230_E 1.415 0.92
649_A 732_V 1.414 0.92
21_Y 31_G 1.411 0.92
700_E 782_L 1.407 0.92
205_I 208_L 1.401 0.92
362_V 498_L 1.396 0.92
144_D 157_D 1.387 0.91
479_K 483_D 1.387 0.91
491_I 497_R 1.378 0.91
646_V 685_V 1.377 0.91
277_I 305_I 1.374 0.91
73_V 85_V 1.374 0.91
99_R 515_D 1.368 0.91
76_K 153_E 1.363 0.90
381_E 482_L 1.362 0.90
239_K 329_S 1.356 0.90
696_L 732_V 1.353 0.90
30_V 337_L 1.351 0.90
785_G 836_L 1.349 0.90
651_A 731_K 1.348 0.90
51_A 85_V 1.342 0.89
351_I 355_V 1.336 0.89
698_S 730_D 1.323 0.89
66_S 125_I 1.309 0.88
45_H 97_S 1.306 0.88
459_L 464_Q 1.306 0.88
363_T 498_L 1.304 0.88
390_I 398_R 1.303 0.88
172_S 181_N 1.3 0.87
244_A 321_Y 1.296 0.87
183_P 334_M 1.294 0.87
277_I 292_L 1.294 0.87
484_Q 487_E 1.289 0.87
64_K 76_K 1.289 0.87
191_I 194_C 1.288 0.87
365_R 505_E 1.287 0.87
475_L 479_K 1.284 0.87
74_I 149_Y 1.28 0.86
194_C 351_I 1.276 0.86
503_R 507_E 1.268 0.86
325_Q 328_V 1.268 0.86
384_A 478_Y 1.266 0.85
545_H 579_D 1.265 0.85
389_N 450_L 1.259 0.85
242_I 519_T 1.253 0.85
244_A 326_L 1.253 0.85
800_D 832_N 1.252 0.85
200_D 204_S 1.251 0.85
222_I 242_I 1.246 0.84
352_A 356_R 1.241 0.84
495_A 499_M 1.239 0.84
408_T 412_A 1.236 0.84
400_A 409_A 1.235 0.83
648_M 685_V 1.232 0.83
774_I 780_N 1.23 0.83
536_Q 586_T 1.229 0.83
362_V 505_E 1.227 0.83
359_R 498_L 1.226 0.83
135_M 159_M 1.224 0.83
297_D 301_M 1.217 0.82
367_I 371_R 1.216 0.82
197_Y 352_A 1.216 0.82
772_I 775_K 1.21 0.82
192_N 514_G 1.209 0.82
756_T 760_E 1.207 0.82
756_T 761_Y 1.205 0.81
62_A 126_R 1.203 0.81
248_V 317_L 1.202 0.81
396_L 440_E 1.202 0.81
226_R 523_A 1.199 0.81
776_V 780_N 1.198 0.81
55_L 68_R 1.197 0.81
366_T 491_I 1.196 0.81
593_F 599_V 1.196 0.81
205_I 209_M 1.195 0.81
647_F 711_V 1.194 0.81
55_L 72_D 1.188 0.80
541_V 604_V 1.188 0.80
642_E 661_T 1.188 0.80
777_T 820_Q 1.187 0.80
401_P 405_E 1.186 0.80
404_A 408_T 1.185 0.80
595_S 630_R 1.184 0.80
181_N 331_G 1.182 0.80
725_R 769_K 1.175 0.79
50_Y 53_N 1.173 0.79
64_K 152_T 1.171 0.79
478_Y 482_L 1.168 0.78
276_K 279_E 1.167 0.78
295_E 302_R 1.164 0.78
486_A 490_R 1.163 0.78
799_T 825_I 1.163 0.78
14_E 18_K 1.159 0.78
295_E 304_V 1.157 0.78
379_I 457_A 1.156 0.77
748_T 783_V 1.151 0.77
248_V 259_I 1.15 0.77
487_E 494_S 1.145 0.77
57_N 69_V 1.144 0.76
649_A 723_G 1.142 0.76
491_I 498_L 1.142 0.76
647_F 688_T 1.141 0.76
19_S 23_D 1.139 0.76
26_M 30_V 1.139 0.76
293_R 306_E 1.137 0.76
610_A 614_A 1.137 0.76
546_Q 573_K 1.132 0.75
289_I 305_I 1.132 0.75
274_I 303_I 1.132 0.75
441_F 448_Y 1.127 0.75
384_A 475_L 1.12 0.74
601_S 664_N 1.118 0.74
747_A 833_V 1.114 0.74
226_R 229_I 1.114 0.74
406_A 467_T 1.108 0.73
746_T 761_Y 1.107 0.73
256_R 308_K 1.105 0.73
657_K 713_A 1.104 0.73
380_L 481_L 1.103 0.73
106_Q 617_R 1.103 0.73
490_R 493_G 1.097 0.72
163_I 191_I 1.097 0.72
274_I 292_L 1.094 0.72
229_I 240_V 1.093 0.72
134_L 161_T 1.092 0.72
333_N 344_I 1.087 0.71
648_M 682_L 1.083 0.71
483_D 487_E 1.083 0.71
136_A 358_R 1.082 0.71
233_Y 347_L 1.081 0.71
658_T 663_F 1.076 0.70
335_V 344_I 1.075 0.70
792_C 810_S 1.075 0.70
258_T 306_E 1.072 0.70
197_Y 348_K 1.069 0.69
675_K 718_T 1.067 0.69
584_A 604_V 1.067 0.69
168_V 191_I 1.066 0.69
349_D 352_A 1.065 0.69
487_E 501_V 1.063 0.69
220_A 245_R 1.059 0.68
396_L 455_A 1.058 0.68
92_M 98_L 1.057 0.68
474_L 498_L 1.056 0.68
162_K 358_R 1.053 0.68
245_R 263_E 1.053 0.68
37_V 345_M 1.05 0.67
695_M 744_I 1.05 0.67
383_L 474_L 1.047 0.67
652_N 679_G 1.046 0.67
22_L 149_Y 1.043 0.67
693_E 763_T 1.042 0.66
339_H 356_R 1.04 0.66
249_E 260_I 1.036 0.66
772_I 818_N 1.033 0.65
405_E 409_A 1.033 0.65
411_V 455_A 1.033 0.65
378_H 451_T 1.032 0.65
700_E 730_D 1.032 0.65
598_R 626_E 1.031 0.65
698_S 732_V 1.028 0.65
743_A 755_R 1.027 0.65
507_E 511_E 1.024 0.64
663_F 673_A 1.023 0.64
16_E 20_S 1.022 0.64
231_E 234_R 1.021 0.64
246_A 261_V 1.021 0.64
31_G 171_S 1.016 0.64
130_I 163_I 1.016 0.64
113_D 270_K 1.009 0.63
392_P 395_E 1.007 0.63
497_R 501_V 1.006 0.62
207_G 210_E 1.004 0.62
25_A 69_V 1 0.62
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1zvuA10.79771000.3Contact Map0.68
4i3hA20.57141000.42Contact Map0.709
2inrA10.56461000.422Contact Map0.681
2novA40.56691000.426Contact Map0.746
2xcsB20.55891000.43Contact Map0.79
2xkjE10.56571000.436Contact Map0.707
3lpxA20.57141000.436Contact Map0.811
3ilwA20.53141000.44Contact Map0.756
4gfhA20.55891000.568Contact Map0.505
3l4jA10.5521000.57Contact Map0.496
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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