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OPENSEQ.org
KPYK1 - Pyruvate kinase I
UniProt: P0AD61 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10804
Length: 470 (469)
Sequences: 2150
Seq/Len: 4.58
KPYK1
Paralog alert: 0.31 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: KPYK1 KPYK2
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
219_S 237_S 4.493 1.00
135_T 145_K 4.366 1.00
21_A 56_K 4.189 1.00
231_D 266_K 4.134 1.00
133_E 145_K 3.551 1.00
44_G 48_Q 3.466 1.00
230_F 263_M 3.381 1.00
8_C 28_M 3.263 1.00
17_E 52_N 3.204 1.00
201_I 236_A 3.143 1.00
28_M 31_M 3.097 1.00
227_L 259_A 3.028 1.00
200_V 219_S 2.97 1.00
65_L 181_G 2.97 1.00
276_T 306_T 2.959 1.00
78_E 102_N 2.897 1.00
19_M 22_K 2.83 1.00
204_R 217_I 2.823 1.00
16_S 19_M 2.668 1.00
24_L 61_A 2.662 1.00
62_A 410_V 2.617 1.00
90_T 143_I 2.607 1.00
42_E 46_R 2.537 1.00
91_F 106_V 2.476 1.00
78_E 100_I 2.47 1.00
133_E 147_L 2.408 1.00
255_E 334_R 2.379 1.00
136_A 143_I 2.332 1.00
200_V 217_I 2.307 1.00
44_G 184_Q 2.288 1.00
19_M 322_L 2.233 1.00
86_K 89_Q 2.166 1.00
90_T 145_K 2.139 1.00
47_I 184_Q 2.131 1.00
90_T 135_T 2.128 1.00
89_Q 103_S 2.09 1.00
178_L 189_V 2.036 1.00
286_K 319_K 2.02 1.00
251_I 259_A 2.013 1.00
372_P 447_D 2 1.00
76_K 155_N 1.985 1.00
10_I 20_L 1.946 1.00
197_R 232_E 1.944 1.00
254_E 330_T 1.931 1.00
93_F 132_M 1.922 1.00
198_S 202_E 1.898 1.00
235_E 270_A 1.894 1.00
309_V 332_C 1.893 1.00
400_T 406_A 1.883 1.00
67_T 71_E 1.88 1.00
125_V 152_L 1.88 1.00
112_G 115_T 1.87 1.00
91_F 144_C 1.87 1.00
21_A 25_D 1.861 1.00
51_R 54_M 1.854 1.00
17_E 49_N 1.853 1.00
218_I 239_G 1.847 1.00
240_I 263_M 1.828 1.00
41_A 44_G 1.825 1.00
261_K 304_D 1.82 1.00
375_V 449_V 1.813 1.00
85_L 146_V 1.81 1.00
15_E 46_R 1.8 1.00
230_F 234_L 1.787 1.00
53_V 57_T 1.778 1.00
29_N 59_K 1.768 1.00
216_H 408_Q 1.767 1.00
33_L 47_I 1.76 1.00
435_E 439_Q 1.753 1.00
47_I 63_I 1.737 1.00
25_D 57_T 1.722 0.99
182_C 215_I 1.713 0.99
175_K 202_E 1.712 0.99
77_L 154_E 1.708 0.99
22_K 25_D 1.705 0.99
198_S 201_I 1.702 0.99
122_T 131_G 1.702 0.99
205_E 209_A 1.694 0.99
118_S 121_N 1.694 0.99
38_G 43_H 1.678 0.99
371_A 450_V 1.673 0.99
119_V 137_I 1.658 0.99
406_A 419_L 1.637 0.99
329_A 333_E 1.626 0.99
185_G 404_K 1.602 0.99
378_T 398_A 1.591 0.99
179_I 210_H 1.586 0.99
8_C 31_M 1.586 0.99
173_K 177_D 1.571 0.99
52_N 56_K 1.568 0.99
116_D 166_A 1.55 0.99
17_E 56_K 1.549 0.99
105_M 141_K 1.534 0.98
264_I 276_T 1.528 0.98
320_Y 323_E 1.52 0.98
81_N 151_D 1.517 0.98
176_Q 179_I 1.513 0.98
172_E 176_Q 1.509 0.98
7_V 30_V 1.506 0.98
89_Q 146_V 1.501 0.98
7_V 275_I 1.488 0.98
197_R 236_A 1.484 0.98
256_V 296_G 1.474 0.98
409_L 412_S 1.473 0.98
17_E 53_V 1.47 0.98
92_T 141_K 1.466 0.98
302_I 332_C 1.456 0.98
365_T 465_A 1.452 0.98
138_E 141_K 1.449 0.98
24_L 53_V 1.445 0.98
253_V 327_I 1.441 0.97
92_T 143_I 1.434 0.97
164_S 225_E 1.423 0.97
31_M 50_L 1.418 0.97
122_T 133_E 1.409 0.97
74_T 130_I 1.404 0.97
179_I 209_A 1.389 0.97
191_A 203_I 1.388 0.97
218_I 275_I 1.38 0.97
285_I 313_G 1.378 0.96
84_S 151_D 1.371 0.96
282_D 286_K 1.365 0.96
4_T 302_I 1.355 0.96
125_V 132_M 1.352 0.96
223_N 226_G 1.349 0.96
132_M 144_C 1.346 0.96
114_T 142_V 1.342 0.96
262_M 266_K 1.333 0.96
83_V 103_S 1.326 0.95
14_T 23_M 1.303 0.95
68_K 71_E 1.302 0.95
96_D 105_M 1.299 0.95
44_G 183_E 1.295 0.94
63_I 186_V 1.295 0.94
234_L 266_K 1.291 0.94
22_K 322_L 1.286 0.94
21_A 57_T 1.283 0.94
121_N 163_V 1.281 0.94
33_L 50_L 1.281 0.94
137_I 142_V 1.278 0.94
26_A 326_S 1.263 0.93
117_L 142_V 1.261 0.93
175_K 179_I 1.26 0.93
259_A 263_M 1.26 0.93
323_E 326_S 1.256 0.93
295_A 331_I 1.255 0.93
80_G 154_E 1.248 0.93
48_Q 52_N 1.247 0.93
87_A 149_N 1.246 0.93
23_M 325_V 1.235 0.92
19_M 23_M 1.232 0.92
326_S 330_T 1.218 0.92
97_K 111_E 1.215 0.92
436_L 439_Q 1.203 0.91
45_Q 49_N 1.202 0.91
47_I 180_F 1.196 0.91
231_D 234_L 1.19 0.91
182_C 207_L 1.188 0.90
10_I 33_L 1.186 0.90
280_M 298_V 1.185 0.90
398_A 409_L 1.183 0.90
448_V 469_V 1.177 0.90
40_Y 180_F 1.176 0.90
33_L 186_V 1.175 0.90
181_G 186_V 1.172 0.90
111_E 114_T 1.17 0.90
30_V 64_L 1.169 0.89
39_D 42_E 1.169 0.89
15_E 49_N 1.162 0.89
95_T 114_T 1.162 0.89
224_Q 228_N 1.159 0.89
86_K 149_N 1.151 0.89
372_P 444_H 1.151 0.89
2_K 329_A 1.141 0.88
45_Q 48_Q 1.14 0.88
4_T 332_C 1.139 0.88
16_S 23_M 1.137 0.88
398_A 415_V 1.134 0.88
71_E 223_N 1.132 0.87
77_L 91_F 1.129 0.87
10_I 46_R 1.128 0.87
376_V 387_V 1.125 0.87
64_L 190_A 1.125 0.87
438_L 445_K 1.117 0.86
365_T 467_V 1.111 0.86
126_D 129_L 1.111 0.86
96_D 140_N 1.11 0.86
160_L 163_V 1.109 0.86
76_K 100_I 1.109 0.86
16_S 49_N 1.099 0.85
160_L 165_I 1.097 0.85
14_T 19_M 1.094 0.85
18_E 21_A 1.082 0.84
43_H 46_R 1.08 0.84
40_Y 177_D 1.074 0.83
30_V 308_A 1.072 0.83
357_A 365_T 1.068 0.83
175_K 206_H 1.065 0.83
197_R 201_I 1.064 0.83
443_A 470_L 1.063 0.82
212_G 215_I 1.059 0.82
322_L 326_S 1.056 0.82
388_R 412_S 1.055 0.82
113_F 142_V 1.053 0.82
207_L 215_I 1.052 0.82
436_L 440_S 1.052 0.82
266_K 270_A 1.048 0.81
90_T 136_A 1.046 0.81
65_L 71_E 1.044 0.81
74_T 153_G 1.043 0.81
75_M 107_A 1.042 0.81
282_D 313_G 1.041 0.81
83_V 152_L 1.039 0.80
79_G 83_V 1.037 0.80
183_E 210_H 1.035 0.80
20_L 23_M 1.034 0.80
101_G 106_V 1.034 0.80
258_F 303_L 1.032 0.80
405_T 409_L 1.024 0.79
68_K 173_K 1.024 0.79
65_L 186_V 1.021 0.79
360_R 364_E 1.021 0.79
20_L 53_V 1.017 0.78
373_L 397_L 1.014 0.78
376_V 383_S 1.009 0.78
371_A 467_V 1.009 0.78
77_L 153_G 1.009 0.78
55_S 403_E 1.009 0.78
10_I 14_T 1.008 0.78
201_I 205_E 1.005 0.77
453_S 456_L 1 0.77
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2e28A10.99571000.13Contact Map0.708
3t05A40.99361000.13Contact Map0.798
1e0tA40.99571000.133Contact Map0.786
3gr4A40.99791000.133Contact Map0.787
1a3wA20.99791000.134Contact Map0.745
3gg8A40.99571000.137Contact Map0.84
3hqnD20.99571000.138Contact Map0.772
4drsA20.99791000.14Contact Map0.764
3khdA40.99361000.14Contact Map0.774
3qtgA20.94471000.178Contact Map0.708
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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