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OPENSEQ.org
MBHM - Hydrogenase-2 large chain
UniProt: P0ACE0 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11801
Length: 567 (561)
Sequences: 620
Seq/Len: 1.11
MBHM
Paralog alert: 0.59 [within 20: 0.04] - ratio of genomes with paralogs
Cluster includes: MBHL MBHM
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
53_A 493_I 4.472 1.00
262_T 429_T 4.187 1.00
489_K 494_S 3.97 1.00
45_V 493_I 2.776 1.00
45_V 53_A 2.612 1.00
155_I 158_L 2.556 1.00
382_N 431_H 2.536 1.00
121_S 184_N 2.531 0.99
130_A 186_I 2.413 0.99
255_E 433_C 2.387 0.99
326_G 371_Y 2.375 0.99
337_K 352_A 2.354 0.99
308_D 311_S 2.353 0.99
30_K 515_V 2.345 0.99
506_S 518_Y 2.34 0.99
183_V 186_I 2.299 0.99
200_D 249_K 2.292 0.99
395_Q 399_N 2.277 0.99
248_D 440_Q 2.231 0.98
369_T 507_G 2.22 0.98
303_Y 375_V 2.167 0.98
118_D 173_G 2.121 0.98
94_I 439_L 2.114 0.98
152_Q 156_K 2.086 0.97
285_V 483_S 2.069 0.97
156_K 160_A 2.06 0.97
149_T 153_N 2.058 0.97
101_T 254_V 2.015 0.96
529_D 532_K 2.013 0.96
502_S 547_M 1.969 0.96
369_T 510_N 1.959 0.96
112_S 425_I 1.95 0.96
357_S 360_G 1.906 0.95
374_T 517_P 1.857 0.94
273_L 422_L 1.845 0.94
113_A 117_V 1.834 0.93
30_K 523_V 1.755 0.91
45_V 57_V 1.75 0.91
93_N 442_Q 1.736 0.90
117_V 187_A 1.722 0.90
113_A 187_A 1.71 0.90
118_D 184_N 1.701 0.89
510_N 514_D 1.68 0.88
387_L 399_N 1.676 0.88
470_K 487_V 1.668 0.88
367_S 507_G 1.651 0.87
448_T 452_K 1.647 0.87
119_I 173_G 1.636 0.87
342_Q 348_T 1.624 0.86
102_H 106_V 1.623 0.86
314_P 389_A 1.615 0.86
382_N 476_E 1.602 0.85
549_C 552_H 1.589 0.84
53_A 57_V 1.556 0.83
60_I 498_A 1.545 0.82
7_I 28_V 1.517 0.80
400_E 408_L 1.513 0.80
546_C 549_C 1.511 0.80
251_S 437_D 1.49 0.79
515_V 520_Q 1.487 0.79
19_I 540_I 1.482 0.78
507_G 510_N 1.463 0.77
173_G 184_N 1.451 0.76
380_L 471_G 1.447 0.76
208_L 243_I 1.445 0.76
560_E 563_S 1.44 0.75
337_K 350_I 1.436 0.75
252_D 256_Q 1.427 0.74
19_I 33_A 1.426 0.74
285_V 474_F 1.421 0.74
300_P 386_K 1.413 0.73
387_L 398_L 1.406 0.73
349_T 497_Q 1.405 0.73
180_P 183_V 1.394 0.72
421_T 539_T 1.386 0.71
202_N 212_T 1.38 0.71
150_K 153_N 1.378 0.71
208_L 443_Y 1.376 0.71
546_C 552_H 1.374 0.70
279_A 517_P 1.36 0.69
298_L 438_I 1.359 0.69
228_N 231_G 1.354 0.69
153_N 156_K 1.343 0.68
95_I 208_L 1.342 0.68
244_K 248_D 1.342 0.68
486_M 496_Y 1.34 0.68
22_E 30_K 1.334 0.67
343_A 347_G 1.333 0.67
255_E 437_D 1.32 0.66
118_D 121_S 1.314 0.65
133_M 183_V 1.313 0.65
16_H 63_V 1.313 0.65
392_E 396_N 1.313 0.65
303_Y 315_I 1.312 0.65
153_N 157_D 1.312 0.65
244_K 440_Q 1.309 0.65
100_T 435_L 1.309 0.65
365_V 547_M 1.308 0.65
243_I 247_I 1.302 0.64
86_V 445_A 1.294 0.64
468_E 489_K 1.289 0.63
414_E 417_Q 1.286 0.63
75_A 486_M 1.28 0.62
227_I 450_I 1.276 0.62
555_D 559_N 1.275 0.62
67_T 96_L 1.271 0.62
326_G 370_F 1.266 0.61
342_Q 347_G 1.255 0.60
79_A 195_L 1.254 0.60
38_W 43_E 1.254 0.60
101_T 250_L 1.254 0.60
244_K 444_S 1.253 0.60
79_A 488_I 1.25 0.60
229_L 450_I 1.247 0.59
317_S 472_V 1.242 0.59
278_G 282_Y 1.24 0.59
46_K 490_D 1.233 0.58
72_S 238_E 1.227 0.58
373_K 514_D 1.225 0.57
97_A 439_L 1.223 0.57
508_P 548_A 1.222 0.57
196_E 249_K 1.214 0.56
121_S 173_G 1.212 0.56
493_I 521_S 1.198 0.55
35_G 365_V 1.193 0.54
119_I 184_N 1.191 0.54
201_A 250_L 1.19 0.54
136_G 140_W 1.189 0.54
7_I 536_V 1.188 0.54
506_S 550_A 1.187 0.54
77_E 92_R 1.186 0.54
500_V 503_T 1.184 0.54
72_S 473_G 1.183 0.53
134_L 137_V 1.182 0.53
57_V 97_A 1.181 0.53
210_G 476_E 1.174 0.53
438_I 441_N 1.174 0.53
399_N 403_A 1.172 0.52
228_N 232_L 1.171 0.52
46_K 489_K 1.167 0.52
413_L 417_Q 1.164 0.52
391_R 394_T 1.16 0.51
409_T 417_Q 1.154 0.51
65_T 106_V 1.153 0.51
306_N 373_K 1.151 0.50
487_V 494_S 1.151 0.50
101_T 432_C 1.15 0.50
10_V 334_S 1.142 0.50
95_I 209_G 1.141 0.49
57_V 547_M 1.138 0.49
75_A 183_V 1.137 0.49
119_I 134_L 1.13 0.48
424_R 504_W 1.13 0.48
94_I 556_A 1.125 0.48
21_C 166_I 1.116 0.47
386_K 394_T 1.112 0.47
16_H 111_L 1.111 0.47
120_T 160_A 1.11 0.47
128_T 145_P 1.103 0.46
153_N 160_A 1.103 0.46
5_I 295_G 1.103 0.46
179_L 260_V 1.101 0.46
194_A 253_F 1.101 0.46
139_T 256_Q 1.1 0.46
251_S 255_E 1.099 0.45
90_Y 442_Q 1.098 0.45
82_I 315_I 1.095 0.45
121_S 534_L 1.094 0.45
133_M 186_I 1.094 0.45
134_L 140_W 1.094 0.45
202_N 205_V 1.091 0.45
43_E 46_K 1.091 0.45
193_Q 422_L 1.09 0.45
8_D 16_H 1.088 0.44
47_N 493_I 1.087 0.44
131_S 145_P 1.084 0.44
287_E 299_F 1.083 0.44
27_V 524_G 1.08 0.44
4_R 358_D 1.079 0.44
283_L 481_M 1.079 0.44
63_V 548_A 1.079 0.44
105_I 250_L 1.078 0.44
369_T 374_T 1.076 0.43
8_D 18_R 1.076 0.43
483_S 504_W 1.07 0.43
57_V 475_L 1.069 0.43
88_A 91_I 1.062 0.42
97_A 442_Q 1.06 0.42
94_I 139_T 1.059 0.42
433_C 437_D 1.059 0.42
78_S 527_V 1.058 0.42
494_S 553_V 1.056 0.41
200_D 203_R 1.056 0.41
254_V 432_C 1.054 0.41
251_S 436_Q 1.053 0.41
16_H 500_V 1.053 0.41
453_G 539_T 1.052 0.41
16_H 478_P 1.052 0.41
127_P 152_Q 1.047 0.41
561_V 565_K 1.047 0.41
558_G 561_V 1.046 0.41
94_I 204_V 1.046 0.41
496_Y 516_G 1.045 0.40
88_A 167_F 1.04 0.40
195_L 421_T 1.04 0.40
229_L 455_H 1.038 0.40
213_P 424_R 1.038 0.40
228_N 242_Y 1.036 0.40
205_V 212_T 1.036 0.40
440_Q 444_S 1.034 0.39
132_E 415_V 1.034 0.39
245_S 452_K 1.033 0.39
398_L 402_V 1.032 0.39
376_E 525_T 1.031 0.39
376_E 543_F 1.031 0.39
107_H 500_V 1.03 0.39
146_E 150_K 1.029 0.39
424_R 519_E 1.026 0.39
298_L 457_T 1.026 0.39
120_T 124_Q 1.024 0.39
380_L 426_I 1.024 0.39
183_V 264_V 1.024 0.39
109_Y 253_F 1.021 0.38
26_G 237_L 1.021 0.38
141_H 558_G 1.02 0.38
244_K 443_Y 1.018 0.38
91_I 247_I 1.017 0.38
103_D 503_T 1.017 0.38
101_T 439_L 1.016 0.38
16_H 113_A 1.016 0.38
280_V 307_A 1.015 0.38
321_E 371_Y 1.015 0.38
5_I 328_Q 1.015 0.38
266_A 392_E 1.015 0.38
279_A 520_Q 1.014 0.38
333_H 380_L 1.014 0.38
204_V 250_L 1.011 0.37
116_W 425_I 1.011 0.37
128_T 312_Y 1.01 0.37
71_S 96_L 1.008 0.37
113_A 258_Y 1.006 0.37
553_V 561_V 1.005 0.37
151_V 494_S 1.004 0.37
67_T 99_H 1.003 0.37
60_I 491_G 1.003 0.37
23_I 385_V 1.002 0.37
444_S 448_T 1.002 0.37
77_E 82_I 1.001 0.36
10_V 19_I 1.001 0.36
103_D 500_V 1.001 0.36
96_L 476_E 1 0.36
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3ayxA20.95241000.195Contact Map0.62
3myrB40.95061000.196Contact Map0.595
1yqwQ30.9331000.208Contact Map0.619
3uqyL20.95061000.228Contact Map0.623
1e3dB20.92951000.238Contact Map0.569
1wuiL10.9331000.244Contact Map0.603
1yq9H20.9331000.269Contact Map0.643
3ze9B10.83071000.375Contact Map0.613
4kn9L20.83771000.378Contact Map0.636
3zfsA10.70021000.537Contact Map0.047
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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