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OPENSEQ.org
PCNB - Poly(A) polymerase
UniProt: P0ABF1 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10690
Length: 465 (415)
Sequences: 574
Seq/Len: 1.38
PCNB
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
401_Y 424_G 2.959 1.00
108_L 121_A 2.848 1.00
199_E 233_R 2.687 1.00
186_D 193_R 2.528 1.00
99_C 109_A 2.523 1.00
165_I 192_I 2.403 1.00
34_R 41_R 2.281 0.99
229_E 233_R 2.201 0.99
163_F 212_A 2.187 0.99
389_W 426_F 2.173 0.99
399_A 402_D 2.052 0.98
52_M 172_V 2.031 0.98
34_R 40_S 2.012 0.98
61_E 86_N 1.977 0.97
298_V 302_T 1.948 0.97
228_A 269_Y 1.867 0.96
202_Y 227_T 1.859 0.96
408_A 420_V 1.778 0.94
276_F 321_M 1.761 0.94
209_M 271_L 1.761 0.94
40_S 175_F 1.751 0.94
106_F 135_T 1.744 0.94
91_Q 94_K 1.716 0.93
109_A 121_A 1.706 0.93
101_L 109_A 1.705 0.93
405_A 420_V 1.702 0.92
182_G 185_K 1.696 0.92
74_L 187_L 1.692 0.92
372_D 396_K 1.655 0.91
429_S 437_M 1.612 0.90
322_F 356_A 1.574 0.88
267_C 280_T 1.566 0.88
60_Y 86_N 1.563 0.88
202_Y 230_P 1.543 0.87
135_T 242_P 1.538 0.86
88_T 91_Q 1.527 0.86
191_V 224_S 1.51 0.85
34_R 175_F 1.503 0.85
224_S 227_T 1.5 0.84
53_Y 174_D 1.5 0.84
199_E 234_L 1.499 0.84
437_M 441_L 1.491 0.84
265_L 269_Y 1.459 0.82
254_L 279_I 1.457 0.82
238_L 274_P 1.432 0.81
279_I 321_M 1.43 0.80
385_G 388_A 1.428 0.80
230_P 234_L 1.422 0.80
109_A 343_D 1.413 0.79
41_R 175_F 1.407 0.79
40_S 53_Y 1.396 0.78
255_Q 313_N 1.394 0.78
93_R 99_C 1.391 0.78
83_V 118_I 1.389 0.78
121_A 125_G 1.383 0.77
55_L 62_A 1.37 0.76
135_T 210_L 1.369 0.76
34_R 53_Y 1.369 0.76
88_T 223_I 1.362 0.76
193_R 224_S 1.355 0.75
53_Y 175_F 1.345 0.74
110_H 344_A 1.341 0.74
193_R 226_E 1.34 0.74
192_I 375_Q 1.322 0.73
92_V 111_V 1.321 0.72
197_N 200_T 1.319 0.72
374_W 377_Q 1.307 0.71
238_L 275_L 1.306 0.71
255_Q 314_P 1.304 0.71
202_Y 242_P 1.302 0.71
410_V 416_L 1.292 0.70
217_A 256_A 1.277 0.69
64_L 182_G 1.273 0.68
191_V 400_A 1.271 0.68
425_E 433_D 1.268 0.68
257_G 303_D 1.267 0.68
105_R 110_H 1.261 0.67
109_A 194_L 1.26 0.67
430_A 433_D 1.256 0.67
99_C 121_A 1.255 0.67
385_G 419_L 1.254 0.67
168_L 395_P 1.246 0.66
52_M 174_D 1.238 0.65
356_A 380_M 1.237 0.65
44_I 49_L 1.235 0.65
52_M 176_T 1.234 0.65
260_Y 296_E 1.229 0.64
56_N 171_S 1.226 0.64
85_T 96_F 1.208 0.63
187_L 190_G 1.207 0.62
437_M 440_E 1.205 0.62
79_K 168_L 1.203 0.62
40_S 43_D 1.2 0.62
222_R 265_L 1.193 0.61
190_G 227_T 1.188 0.61
305_R 398_R 1.184 0.60
227_T 287_G 1.184 0.60
52_M 154_I 1.179 0.60
59_G 235_A 1.175 0.59
419_L 422_W 1.173 0.59
185_K 189_D 1.167 0.59
79_K 96_F 1.161 0.58
142_N 240_D 1.158 0.58
92_V 422_W 1.157 0.58
433_D 436_G 1.151 0.57
237_L 240_D 1.148 0.57
213_V 248_E 1.146 0.56
402_D 405_A 1.143 0.56
54_R 98_N 1.141 0.56
45_S 48_A 1.138 0.56
101_L 111_V 1.136 0.55
69_V 177_V 1.133 0.55
172_V 175_F 1.132 0.55
125_G 145_L 1.131 0.55
366_L 369_L 1.129 0.55
278_T 282_Y 1.127 0.55
410_V 413_N 1.126 0.54
429_S 433_D 1.125 0.54
432_P 436_G 1.118 0.54
56_N 173_A 1.117 0.54
355_E 390_K 1.117 0.54
83_V 322_F 1.111 0.53
270_H 273_Q 1.104 0.52
192_I 260_Y 1.103 0.52
53_Y 57_K 1.103 0.52
267_C 283_F 1.099 0.52
229_E 232_P 1.099 0.52
39_I 177_V 1.098 0.52
213_V 265_L 1.097 0.52
85_T 92_V 1.097 0.52
213_V 223_I 1.093 0.51
401_Y 405_A 1.088 0.51
398_R 402_D 1.088 0.51
253_L 266_L 1.082 0.50
254_L 295_I 1.08 0.50
394_H 398_R 1.075 0.49
304_T 308_N 1.074 0.49
381_S 409_E 1.074 0.49
134_R 139_R 1.07 0.49
171_S 174_D 1.069 0.49
432_P 435_K 1.067 0.49
153_S 261_E 1.065 0.48
53_Y 172_V 1.063 0.48
60_Y 188_K 1.063 0.48
110_H 397_F 1.06 0.48
168_L 232_P 1.06 0.48
140_G 146_L 1.059 0.48
433_D 439_N 1.059 0.48
65_V 106_F 1.058 0.48
363_P 427_Q 1.056 0.47
85_T 402_D 1.056 0.47
411_E 414_A 1.054 0.47
29_V 411_E 1.054 0.47
103_G 110_H 1.053 0.47
148_D 377_Q 1.051 0.47
434_Q 437_M 1.05 0.47
45_S 210_L 1.05 0.47
96_F 111_V 1.047 0.47
292_E 353_L 1.046 0.46
60_Y 170_Y 1.046 0.46
193_R 217_A 1.044 0.46
190_G 222_R 1.043 0.46
431_P 436_G 1.042 0.46
436_G 440_E 1.042 0.46
121_A 315_A 1.04 0.46
109_A 175_F 1.035 0.45
159_Q 181_V 1.035 0.45
52_M 175_F 1.035 0.45
52_M 64_L 1.035 0.45
254_L 263_Y 1.034 0.45
94_K 390_K 1.03 0.45
167_S 434_Q 1.03 0.45
61_E 233_R 1.029 0.45
440_E 443_E 1.028 0.45
102_V 109_A 1.028 0.45
428_V 435_K 1.027 0.45
41_R 53_Y 1.025 0.44
111_V 116_E 1.024 0.44
190_G 389_W 1.022 0.44
185_K 188_K 1.022 0.44
54_R 95_L 1.022 0.44
85_T 247_F 1.019 0.44
79_K 254_L 1.017 0.44
65_V 83_V 1.017 0.44
238_L 399_A 1.017 0.44
232_P 268_E 1.016 0.43
278_T 422_W 1.013 0.43
60_Y 85_T 1.013 0.43
295_I 321_M 1.013 0.43
229_E 261_E 1.012 0.43
220_G 261_E 1.012 0.43
40_S 172_V 1.011 0.43
362_I 394_H 1.007 0.43
422_W 426_F 1.002 0.42
221_M 324_Y 1.001 0.42
186_D 217_A 1 0.42
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3aqlA20.89251000.323Contact Map0.49
1miwA20.81000.6Contact Map0.509
3h38A10.83661000.604Contact Map0.468
1ou5A20.84951000.606Contact Map0.442
1vfgA20.79351000.622Contact Map0.501
2fclA10.31440.70.98Contact Map0.327
4e8jA20.298936.80.98Contact Map0.478
2dulA10.172160.983Contact Map0.629
2l60A10.07749.90.985Contact Map0.505
1eboA60.17859.40.985Contact Map0.698
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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