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OPENSEQ.org
USPE - Universal stress protein E
UniProt: P0AAC0 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11246
Length: 316 (292)
Sequences: 3137
Seq/Len: 10.74
USPE
Paralog alert: 0.38 [within 20: 0.09] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
155_K 191_E 3.201 1.00
114_K 134_L 3.165 1.00
157_L 263_A 3.114 1.00
143_W 297_L 2.996 1.00
94_N 99_A 2.701 1.00
8_L 109_H 2.677 1.00
6_N 34_K 2.668 1.00
268_L 288_V 2.46 1.00
191_E 242_M 2.449 1.00
69_T 73_H 2.391 1.00
193_H 244_H 2.382 1.00
258_A 266_V 2.325 1.00
248_G 253_V 2.291 1.00
112_V 138_C 2.129 1.00
155_K 193_H 2.105 1.00
158_V 177_V 2.089 1.00
36_K 89_K 2.083 1.00
181_I 238_I 2.05 1.00
244_H 257_L 2.03 1.00
6_N 36_K 2.024 1.00
155_K 264_G 1.997 1.00
36_K 87_E 1.967 1.00
76_A 88_I 1.939 1.00
195_V 254_I 1.935 1.00
163_A 272_G 1.935 1.00
292_L 296_L 1.92 1.00
179_E 297_L 1.906 1.00
104_V 112_V 1.878 1.00
34_K 87_E 1.869 1.00
157_L 195_V 1.86 1.00
10_V 100_I 1.831 1.00
76_A 80_L 1.822 1.00
159_A 254_I 1.812 1.00
24_A 28_H 1.795 1.00
7_M 111_L 1.793 1.00
266_V 294_C 1.777 1.00
146_K 295_D 1.756 1.00
258_A 263_A 1.748 1.00
10_V 114_K 1.71 1.00
34_K 85_P 1.667 1.00
33_G 110_D 1.635 1.00
13_P 162_L 1.607 1.00
89_K 103_E 1.598 1.00
157_L 266_V 1.596 1.00
173_N 176_L 1.579 1.00
9_V 21_L 1.559 1.00
194_L 229_M 1.543 1.00
23_R 143_W 1.542 1.00
226_L 230_K 1.541 1.00
266_V 292_L 1.538 1.00
269_G 282_G 1.535 1.00
265_I 297_L 1.51 1.00
12_D 17_D 1.501 1.00
22_R 78_Y 1.489 1.00
136_R 286_E 1.478 1.00
257_L 261_L 1.474 1.00
138_C 142_V 1.465 1.00
37_A 76_A 1.459 1.00
91_V 99_A 1.456 1.00
258_A 294_C 1.451 1.00
40_P 99_A 1.447 1.00
23_R 145_V 1.427 1.00
251_E 287_Q 1.419 1.00
254_I 288_V 1.406 0.99
38_F 103_E 1.389 0.99
250_P 253_V 1.389 0.99
135_L 298_V 1.387 0.99
156_A 184_A 1.367 0.99
14_N 128_T 1.365 0.99
257_L 263_A 1.364 0.99
9_V 24_A 1.364 0.99
259_E 293_R 1.361 0.99
9_V 35_I 1.358 0.99
270_T 275_G 1.352 0.99
270_T 298_V 1.35 0.99
197_A 250_P 1.343 0.99
104_V 109_H 1.329 0.99
111_L 143_W 1.321 0.99
14_N 118_Q 1.313 0.99
27_L 183_L 1.31 0.99
68_R 90_V 1.309 0.99
163_A 173_N 1.296 0.99
36_K 109_H 1.29 0.99
197_A 253_V 1.282 0.99
97_F 133_H 1.281 0.99
181_I 236_F 1.275 0.99
255_P 292_L 1.256 0.99
246_E 253_V 1.232 0.98
158_V 176_L 1.232 0.98
14_N 17_D 1.229 0.98
161_N 272_G 1.222 0.98
159_A 268_L 1.222 0.98
131_D 144_M 1.216 0.98
70_A 74_E 1.215 0.98
134_L 142_V 1.212 0.98
193_H 257_L 1.208 0.98
272_G 275_G 1.205 0.98
26_Y 30_R 1.201 0.98
222_R 226_L 1.191 0.98
8_L 38_F 1.187 0.98
20_A 113_L 1.186 0.98
23_R 295_D 1.186 0.98
112_V 140_S 1.182 0.98
218_N 222_R 1.181 0.98
21_L 37_A 1.175 0.98
38_F 100_I 1.166 0.97
258_A 293_R 1.163 0.97
158_V 180_T 1.161 0.97
255_P 288_V 1.158 0.97
157_L 258_A 1.157 0.97
195_V 257_L 1.154 0.97
73_H 77_K 1.152 0.97
38_F 91_V 1.152 0.97
288_V 296_L 1.15 0.97
101_I 137_K 1.149 0.97
65_I 69_T 1.144 0.97
134_L 138_C 1.139 0.97
28_H 33_G 1.132 0.97
223_G 227_L 1.127 0.97
114_K 131_D 1.126 0.97
255_P 258_A 1.126 0.97
11_I 37_A 1.103 0.96
154_G 190_T 1.1 0.96
118_Q 128_T 1.089 0.96
232_L 236_F 1.085 0.96
77_K 80_L 1.08 0.95
67_Q 70_A 1.078 0.95
157_L 257_L 1.069 0.95
162_L 196_G 1.069 0.95
21_L 25_V 1.068 0.95
39_L 68_R 1.063 0.95
179_E 299_I 1.062 0.95
141_P 300_K 1.055 0.95
104_V 140_S 1.054 0.95
37_A 79_Y 1.05 0.94
275_G 278_A 1.05 0.94
104_V 139_P 1.045 0.94
66_S 70_A 1.043 0.94
182_E 186_Q 1.042 0.94
12_D 128_T 1.032 0.94
36_K 103_E 1.03 0.94
135_L 142_V 1.021 0.93
71_W 75_Q 1.019 0.93
253_V 256_D 1.018 0.93
104_V 138_C 1.018 0.93
216_V 219_D 1.014 0.93
75_Q 78_Y 1.012 0.93
117_H 131_D 1.008 0.93
17_D 118_Q 1.002 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3olqA10.99371000.178Contact Map0.734
3mt0A10.90511000.265Contact Map0.736
3loqA20.85761000.309Contact Map0.711
3cisA80.88611000.329Contact Map0.813
3ab8A20.81961000.361Contact Map0.723
2dumA40.599.90.592Contact Map0.725
1mjhA20.471599.90.615Contact Map0.819
3s3tA80.449499.90.621Contact Map0.713
3tnjA10.455799.90.622Contact Map0.767
3hgmA40.449499.90.626Contact Map0.845
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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