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OPENSEQ.org

PHOU - Phosphate-specific transport system accessory protein PhoU
UniProt: P0A9K7 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10735
Length: 241 (217)
Sequences: 2132
Seq/Len: 9.82

PHOU
Paralog alert: 0.54 [within 20: 0.02] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
29_G 143_L 3.58 1.00
141_D 160_E 3.191 1.00
41_T 45_N 2.698 1.00
100_E 104_D 2.667 1.00
164_V 203_G 2.341 1.00
62_N 104_D 2.248 1.00
32_V 61_V 2.245 1.00
58_D 104_D 2.203 1.00
38_D 53_R 2.199 1.00
33_E 140_H 2.179 1.00
32_V 99_L 2.156 1.00
165_D 204_D 2.131 1.00
161_D 165_D 2.103 1.00
35_Q 61_V 2.079 1.00
165_D 200_E 2.07 1.00
157_I 210_C 2.058 1.00
40_I 132_G 2.031 1.00
62_N 100_E 1.925 1.00
58_D 100_E 1.925 1.00
98_E 208_N 1.901 1.00
61_V 103_G 1.892 1.00
144_D 148_R 1.874 1.00
52_K 114_L 1.824 1.00
151_I 217_V 1.77 1.00
135_T 199_I 1.751 1.00
63_M 67_A 1.741 1.00
24_Q 71_A 1.733 1.00
161_D 200_E 1.713 1.00
141_D 156_R 1.679 1.00
208_N 211_E 1.646 1.00
179_M 186_I 1.642 1.00
98_E 205_R 1.623 1.00
52_K 56_E 1.612 1.00
39_A 106_A 1.581 1.00
58_D 62_N 1.577 1.00
135_T 164_V 1.519 1.00
154_A 214_F 1.495 1.00
37_S 136_I 1.482 1.00
139_L 206_C 1.461 1.00
91_V 216_Y 1.446 1.00
102_I 202_I 1.42 1.00
200_E 204_D 1.413 0.99
134_H 163_K 1.403 0.99
92_I 146_F 1.374 0.99
95_T 209_I 1.362 0.99
38_D 50_L 1.358 0.99
162_K 165_D 1.353 0.99
127_S 174_Q 1.343 0.99
160_E 163_K 1.335 0.99
44_H 129_E 1.326 0.99
38_D 57_G 1.32 0.99
142_V 206_C 1.31 0.99
55_I 58_D 1.31 0.99
65_E 94_K 1.307 0.99
41_T 50_L 1.29 0.99
30_G 33_E 1.288 0.99
161_D 204_D 1.283 0.99
144_D 153_E 1.277 0.99
137_Q 141_D 1.259 0.99
171_I 196_A 1.258 0.99
25_V 95_T 1.253 0.99
177_T 181_E 1.251 0.98
24_Q 27_T 1.245 0.98
108_K 198_S 1.242 0.98
150_D 153_E 1.24 0.98
153_E 156_R 1.238 0.98
42_A 54_V 1.233 0.98
168_Y 196_A 1.203 0.98
138_M 203_G 1.197 0.98
21_I 72_C 1.193 0.98
102_I 139_L 1.191 0.98
145_A 157_I 1.18 0.98
74_R 78_K 1.18 0.98
101_R 201_R 1.178 0.98
141_D 153_E 1.166 0.97
35_Q 57_G 1.164 0.97
48_S 117_F 1.161 0.97
180_M 184_R 1.156 0.97
200_E 203_G 1.154 0.97
27_T 64_M 1.154 0.97
96_I 212_F 1.148 0.97
33_E 37_S 1.131 0.97
72_C 93_S 1.13 0.97
25_V 92_I 1.129 0.97
34_Q 38_D 1.128 0.97
21_I 75_I 1.122 0.96
31_M 60_N 1.121 0.96
51_A 114_L 1.116 0.96
104_D 208_N 1.107 0.96
163_K 166_Q 1.099 0.96
142_V 213_I 1.097 0.96
35_Q 54_V 1.096 0.96
152_D 155_V 1.091 0.96
39_A 113_A 1.085 0.95
14_F 75_I 1.079 0.95
47_D 50_L 1.072 0.95
59_K 62_N 1.067 0.95
30_G 144_D 1.039 0.94
26_M 143_L 1.038 0.94
72_C 96_I 1.023 0.93
166_Q 170_G 1.022 0.93
14_F 79_R 1.02 0.93
53_R 57_G 1.02 0.93
112_T 195_C 1.018 0.93
101_R 204_D 1.014 0.93
101_R 208_N 1.012 0.93
81_P 87_R 1.009 0.93
17_E 71_A 1.008 0.93
144_D 147_A 1.008 0.93
32_V 103_G 1.008 0.93
173_R 181_E 1.002 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1sumB10.95021000.199Contact Map0.748
2i0mA10.89631000.208Contact Map0.749
1xwmA10.90041000.21Contact Map0.688
1t72A60.94191000.265Contact Map0.836
2oltA30.850699.90.521Contact Map0.409
3l39A10.867299.60.675Contact Map0.548
1vctA10.813397.20.851Contact Map0.633
3ci9A20.199212.60.946Contact Map0.333
3bvoA20.29885.80.954Contact Map0.477
1wy1A30.48555.70.954Contact Map0.169

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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