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OPENSEQ.org
RNG - Ribonuclease G
UniProt: P0A9J0 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11299
Length: 489 (486)
Sequences: 1160
Seq/Len: 2.39
RNG
Paralog alert: 0.51 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
282_R 295_D 3.981 1.00
329_Q 363_S 3.955 1.00
230_R 258_E 3.689 1.00
132_F 166_G 3.098 1.00
317_D 356_H 2.895 1.00
66_A 119_L 2.828 1.00
4_E 230_R 2.685 1.00
321_F 356_H 2.682 1.00
449_A 473_V 2.646 1.00
405_C 408_C 2.606 1.00
43_K 102_D 2.577 1.00
288_S 326_E 2.515 1.00
364_L 386_V 2.492 1.00
8_N 234_D 2.463 1.00
6_L 266_I 2.331 1.00
150_E 153_R 2.28 1.00
368_L 377_V 2.276 1.00
296_Q 302_T 2.158 1.00
150_E 176_G 2.113 1.00
262_G 269_L 2.099 1.00
48_R 65_A 2.092 1.00
236_R 259_H 2.09 1.00
279_A 397_I 2.009 0.99
214_A 241_A 1.996 0.99
307_T 320_I 1.994 0.99
111_P 117_A 1.982 0.99
361_L 386_V 1.968 0.99
405_C 412_G 1.894 0.99
104_M 209_L 1.878 0.99
203_P 207_Y 1.826 0.98
160_E 179_E 1.818 0.98
183_A 187_A 1.818 0.98
303_V 331_I 1.815 0.98
73_I 97_V 1.804 0.98
245_F 250_I 1.769 0.98
265_P 273_E 1.743 0.98
20_D 26_E 1.736 0.98
233_V 242_L 1.732 0.98
408_C 412_G 1.727 0.98
291_Y 308_G 1.688 0.97
247_S 254_T 1.673 0.97
243_L 254_T 1.663 0.97
260_Y 265_P 1.656 0.97
363_S 366_Q 1.654 0.97
405_C 410_G 1.65 0.97
27_I 276_I 1.648 0.97
239_Y 243_L 1.631 0.96
356_H 359_R 1.626 0.96
321_F 325_I 1.623 0.96
415_K 423_E 1.608 0.96
246_T 257_L 1.599 0.96
47_S 59_D 1.595 0.96
239_Y 257_L 1.538 0.94
339_N 372_R 1.524 0.94
195_K 199_R 1.512 0.94
329_Q 367_A 1.502 0.93
300_M 385_L 1.5 0.93
108_V 120_T 1.499 0.93
332_A 367_A 1.49 0.93
345_I 385_L 1.485 0.93
240_E 244_E 1.472 0.93
443_Y 476_E 1.463 0.92
379_G 387_E 1.447 0.92
428_I 440_F 1.446 0.92
177_V 181_E 1.445 0.92
158_V 168_F 1.442 0.92
46_V 49_V 1.423 0.91
325_I 363_S 1.421 0.91
43_K 104_M 1.414 0.90
49_V 97_V 1.409 0.90
406_P 414_V 1.402 0.90
141_V 303_V 1.392 0.90
224_A 231_I 1.369 0.88
112_L 392_R 1.366 0.88
346_I 388_M 1.361 0.88
290_G 326_E 1.361 0.88
458_H 465_I 1.357 0.88
166_G 190_K 1.353 0.88
161_Y 190_K 1.35 0.88
188_Y 245_F 1.345 0.87
132_F 163_D 1.337 0.87
234_D 266_I 1.336 0.87
111_P 118_R 1.334 0.87
268_D 273_E 1.324 0.86
23_I 303_V 1.323 0.86
20_D 25_Q 1.317 0.86
427_E 484_F 1.305 0.85
5_L 18_Y 1.304 0.85
132_F 190_K 1.298 0.85
240_E 243_L 1.291 0.84
476_E 479_Y 1.287 0.84
446_P 475_I 1.282 0.84
8_N 15_R 1.277 0.83
439_R 470_Q 1.268 0.83
81_A 84_E 1.265 0.83
446_P 450_E 1.263 0.82
53_M 307_T 1.261 0.82
444_A 473_V 1.261 0.82
361_L 379_G 1.259 0.82
13_E 426_R 1.259 0.82
50_L 53_M 1.254 0.82
130_L 182_L 1.25 0.81
44_G 119_L 1.247 0.81
331_I 346_I 1.244 0.81
366_Q 369_S 1.237 0.81
82_G 91_R 1.225 0.80
285_E 291_Y 1.223 0.79
156_K 160_E 1.221 0.79
130_L 186_A 1.211 0.79
236_R 240_E 1.209 0.78
21_G 231_I 1.209 0.78
42_Y 405_C 1.208 0.78
139_V 167_G 1.205 0.78
305_I 331_I 1.204 0.78
301_T 340_L 1.201 0.78
97_V 101_Q 1.195 0.77
163_D 190_K 1.193 0.77
377_V 388_M 1.191 0.77
198_E 202_R 1.19 0.77
163_D 166_G 1.184 0.76
117_A 316_L 1.183 0.76
134_P 193_W 1.178 0.76
60_I 66_A 1.177 0.76
368_L 375_T 1.177 0.76
24_L 275_E 1.174 0.75
307_T 312_G 1.173 0.75
381_S 387_E 1.171 0.75
407_T 480_N 1.167 0.75
37_I 40_N 1.16 0.74
232_R 266_I 1.158 0.74
335_L 340_L 1.156 0.74
301_T 387_E 1.156 0.74
221_R 350_D 1.156 0.74
428_I 467_V 1.146 0.73
243_L 247_S 1.144 0.73
367_A 370_K 1.139 0.72
439_R 472_K 1.137 0.72
173_A 346_I 1.136 0.72
137_S 374_K 1.136 0.72
231_I 340_L 1.132 0.72
403_N 417_V 1.132 0.72
46_V 381_S 1.129 0.71
152_E 365_E 1.127 0.71
66_A 343_I 1.125 0.71
15_R 426_R 1.124 0.71
45_R 388_M 1.117 0.70
449_A 475_I 1.116 0.70
92_D 96_L 1.113 0.70
380_F 386_V 1.11 0.70
383_L 391_K 1.109 0.69
454_G 457_S 1.107 0.69
421_C 448_V 1.107 0.69
428_I 471_V 1.105 0.69
194_T 241_A 1.105 0.69
424_I 484_F 1.104 0.69
3_A 20_D 1.103 0.69
361_L 381_S 1.102 0.69
470_Q 478_L 1.1 0.69
33_A 210_Y 1.099 0.68
157_V 179_E 1.097 0.68
61_G 64_K 1.095 0.68
373_V 381_S 1.094 0.68
333_R 336_R 1.094 0.68
302_T 343_I 1.091 0.68
212_E 221_R 1.086 0.67
120_T 271_D 1.086 0.67
44_G 59_D 1.086 0.67
15_R 273_E 1.086 0.67
44_G 103_L 1.086 0.67
354_E 357_R 1.085 0.67
113_G 282_R 1.084 0.67
99_Q 397_I 1.082 0.67
17_A 24_L 1.08 0.66
46_V 97_V 1.08 0.66
113_G 295_D 1.079 0.66
124_T 132_F 1.078 0.66
50_L 118_R 1.078 0.66
286_L 290_G 1.075 0.66
203_P 276_I 1.075 0.66
255_S 261_T 1.073 0.66
162_C 300_M 1.069 0.65
44_G 60_I 1.068 0.65
280_L 397_I 1.068 0.65
123_I 196_V 1.066 0.65
264_Q 269_L 1.065 0.65
107_V 177_V 1.06 0.64
69_H 118_R 1.058 0.64
270_F 276_I 1.058 0.64
67_F 72_D 1.057 0.64
83_E 86_K 1.057 0.64
93_I 96_L 1.055 0.64
442_V 452_L 1.052 0.63
321_F 359_R 1.052 0.63
36_G 110_D 1.052 0.63
444_A 449_A 1.051 0.63
24_L 459_S 1.051 0.63
276_I 397_I 1.051 0.63
16_V 343_I 1.05 0.63
6_L 272_V 1.05 0.63
60_I 315_N 1.049 0.63
479_Y 485_D 1.049 0.63
188_Y 210_Y 1.048 0.63
44_G 58_V 1.047 0.63
8_N 426_R 1.042 0.62
107_V 119_L 1.042 0.62
232_R 258_E 1.038 0.62
432_H 469_K 1.036 0.62
43_K 205_T 1.035 0.62
49_V 52_G 1.034 0.62
187_A 244_E 1.034 0.62
320_I 391_K 1.031 0.61
328_T 348_F 1.031 0.61
53_M 316_L 1.029 0.61
330_A 334_Q 1.029 0.61
128_R 250_I 1.025 0.61
271_D 274_N 1.024 0.60
361_L 383_L 1.024 0.60
178_G 181_E 1.019 0.60
141_V 296_Q 1.018 0.60
50_L 307_T 1.015 0.59
340_L 418_E 1.014 0.59
421_C 450_E 1.013 0.59
153_R 157_V 1.009 0.59
142_S 298_E 1.007 0.58
168_F 186_A 1.006 0.58
111_P 212_E 1.005 0.58
29_I 123_I 1.004 0.58
195_K 198_E 1.003 0.58
451_A 478_L 1.002 0.58
179_E 183_A 1 0.58
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2bx2L10.99181000.112Contact Map0.573
3cw2C40.470399.90.85Contact Map0.438
3aevA10.50199.20.92Contact Map0.544
1kl9A10.337497.90.947Contact Map0.542
2ja9A10.335497.80.948Contact Map0.544
2k52A10.163697.60.951Contact Map0.714
1wi5A10.243497.50.951Contact Map0.441
2k4kA10.261897.40.952Contact Map0.639
2cqoA10.239397.30.953Contact Map0.608
2eqsA10.204597.20.954Contact Map0.544
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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