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OPENSEQ.org
SYN - Asparagine--tRNA ligase
UniProt: P0A8M0 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10094
Length: 466 (447)
Sequences: 4797
Seq/Len: 10.73
SYN
Paralog alert: 0.91 [within 20: 0.02] - ratio of genomes with paralogs
Cluster includes: EPMA SYD SYK1 SYK2 SYN
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
155_Q 268_A 5.166 1.00
77_T 96_K 4.977 1.00
151_F 268_A 4.448 1.00
52_A 94_A 4.206 1.00
21_T 77_T 3.92 1.00
153_N 227_Y 3.908 1.00
19_E 77_T 3.822 1.00
150_R 154_E 3.736 1.00
146_Q 150_R 3.563 1.00
75_I 98_E 3.52 1.00
216_G 226_I 3.39 1.00
147_A 272_Y 2.979 1.00
257_A 261_D 2.945 1.00
147_A 276_A 2.911 1.00
19_E 95_S 2.84 1.00
148_L 250_L 2.825 1.00
146_Q 153_N 2.823 1.00
164_L 233_F 2.783 1.00
140_V 446_T 2.757 1.00
395_Q 432_S 2.727 1.00
52_A 76_V 2.48 1.00
384_A 390_I 2.42 1.00
219_Y 431_H 2.41 1.00
145_A 438_F 2.399 1.00
105_D 425_R 2.382 1.00
164_L 230_G 2.373 1.00
20_V 92_I 2.273 1.00
275_K 281_R 2.268 1.00
264_G 310_Q 2.253 1.00
149_H 159_W 2.226 1.00
262_I 432_S 2.174 1.00
144_L 442_I 2.166 1.00
413_N 416_D 2.166 1.00
151_F 155_Q 2.131 1.00
23_R 75_I 2.13 1.00
19_E 79_K 2.109 1.00
150_R 153_N 2.101 1.00
404_D 408_L 2.097 1.00
254_V 257_A 2.091 1.00
79_K 95_S 2.091 1.00
50_V 92_I 2.088 1.00
68_L 74_V 2.056 1.00
151_F 269_M 2.052 1.00
150_R 276_A 2.023 1.00
264_G 268_A 2.021 1.00
259_L 378_A 2.01 1.00
142_H 153_N 1.998 1.00
408_L 414_K 1.993 1.00
178_R 205_E 1.989 1.00
147_A 273_V 1.988 1.00
267_E 310_Q 1.988 1.00
258_N 261_D 1.964 1.00
137_V 448_V 1.925 1.00
400_L 404_D 1.917 1.00
151_F 272_Y 1.915 1.00
405_E 409_E 1.912 1.00
355_V 382_V 1.903 1.00
272_Y 276_A 1.9 1.00
318_T 322_N 1.892 1.00
252_P 434_F 1.884 1.00
270_L 382_V 1.882 1.00
260_N 264_G 1.873 1.00
148_L 269_M 1.849 1.00
81_V 93_Q 1.847 1.00
298_S 301_E 1.808 1.00
22_V 50_V 1.797 1.00
405_E 408_L 1.787 1.00
136_A 140_V 1.781 1.00
311_V 356_V 1.775 1.00
156_G 225_K 1.753 1.00
315_D 318_T 1.749 1.00
404_D 414_K 1.738 1.00
76_V 94_A 1.726 1.00
139_R 158_F 1.723 1.00
157_F 254_V 1.691 1.00
154_E 272_Y 1.686 1.00
17_D 79_K 1.685 1.00
369_R 399_R 1.648 1.00
152_F 250_L 1.623 1.00
143_T 146_Q 1.613 1.00
404_D 418_W 1.588 1.00
26_V 74_V 1.587 1.00
94_A 97_V 1.572 1.00
316_A 356_V 1.566 1.00
274_F 445_V 1.561 1.00
79_K 93_Q 1.548 1.00
21_T 75_I 1.548 1.00
232_T 249_M 1.545 1.00
400_L 425_R 1.538 1.00
338_S 341_H 1.537 1.00
404_D 421_R 1.522 1.00
135_G 139_R 1.52 1.00
369_R 398_E 1.519 1.00
370_L 378_A 1.508 1.00
37_S 61_Y 1.5 1.00
212_G 232_T 1.481 1.00
16_V 82_A 1.479 1.00
318_T 321_E 1.472 1.00
139_R 143_T 1.46 1.00
26_V 39_L 1.449 1.00
132_N 136_A 1.449 1.00
271_K 306_A 1.445 1.00
138_A 456_P 1.442 1.00
160_V 223_L 1.419 1.00
402_V 405_E 1.415 1.00
41_V 74_V 1.413 1.00
395_Q 398_E 1.407 0.99
136_A 139_R 1.405 0.99
22_V 41_V 1.403 0.99
124_V 422_D 1.402 0.99
165_I 206_S 1.394 0.99
282_A 291_R 1.388 0.99
42_Y 47_F 1.374 0.99
282_A 286_K 1.369 0.99
312_D 315_D 1.367 0.99
26_V 68_L 1.367 0.99
312_D 358_N 1.366 0.99
254_V 265_L 1.36 0.99
275_K 279_E 1.359 0.99
250_L 438_F 1.357 0.99
382_V 391_I 1.35 0.99
225_K 254_V 1.349 0.99
294_K 298_S 1.345 0.99
143_T 276_A 1.344 0.99
257_A 432_S 1.341 0.99
80_V 90_F 1.34 0.99
311_V 316_A 1.324 0.99
176_M 182_L 1.317 0.99
159_W 227_Y 1.314 0.99
399_R 402_V 1.312 0.99
152_F 252_P 1.301 0.99
140_V 277_V 1.3 0.99
11_Q 89_Q 1.295 0.99
148_L 438_F 1.29 0.99
138_A 223_L 1.282 0.99
367_Y 394_S 1.269 0.99
39_L 74_V 1.266 0.99
293_D 305_E 1.265 0.99
80_V 92_I 1.264 0.99
422_D 425_R 1.264 0.99
147_A 150_R 1.255 0.99
212_G 249_M 1.253 0.99
148_L 273_V 1.229 0.98
45_S 224_S 1.221 0.98
133_L 448_V 1.218 0.98
371_N 378_A 1.216 0.98
36_I 53_V 1.212 0.98
271_K 281_R 1.21 0.98
285_M 332_Y 1.204 0.98
176_M 463_N 1.202 0.98
214_L 419_W 1.201 0.98
358_N 378_A 1.2 0.98
268_A 271_K 1.191 0.98
144_L 273_V 1.19 0.98
286_K 289_A 1.189 0.98
144_L 445_V 1.188 0.98
319_I 322_N 1.185 0.98
147_A 277_V 1.183 0.98
309_A 319_I 1.181 0.98
358_N 370_L 1.181 0.98
143_T 153_N 1.18 0.98
282_A 293_D 1.179 0.98
315_D 319_I 1.169 0.97
140_V 143_T 1.166 0.97
15_A 18_S 1.166 0.97
369_R 402_V 1.162 0.97
409_E 412_L 1.146 0.97
142_H 146_Q 1.138 0.97
281_R 305_E 1.136 0.97
373_D 376_T 1.133 0.97
35_G 56_N 1.132 0.97
183_D 205_E 1.13 0.97
152_F 269_M 1.129 0.97
63_E 66_L 1.128 0.97
212_G 215_N 1.126 0.97
160_V 219_Y 1.125 0.97
54_I 94_A 1.125 0.97
257_A 262_I 1.124 0.97
22_V 92_I 1.123 0.97
321_E 332_Y 1.12 0.96
297_V 301_E 1.12 0.96
43_D 46_C 1.116 0.96
129_P 138_A 1.108 0.96
266_A 436_L 1.107 0.96
387_I 390_I 1.105 0.96
212_G 230_G 1.104 0.96
271_K 305_E 1.101 0.96
69_T 72_C 1.1 0.96
75_I 100_A 1.099 0.96
261_D 264_G 1.097 0.96
267_E 271_K 1.094 0.96
269_M 273_V 1.066 0.95
39_L 94_A 1.061 0.95
161_S 231_P 1.057 0.95
67_R 103_V 1.055 0.95
428_T 431_H 1.055 0.95
314_T 318_T 1.054 0.95
307_D 310_Q 1.052 0.95
268_A 272_Y 1.05 0.94
406_R 409_E 1.043 0.94
404_D 425_R 1.042 0.94
289_A 447_G 1.04 0.94
270_L 441_L 1.037 0.94
406_R 410_M 1.036 0.94
157_F 225_K 1.035 0.94
68_L 97_V 1.029 0.94
273_V 277_V 1.028 0.94
142_H 227_Y 1.027 0.94
299_R 302_R 1.027 0.94
27_R 42_Y 1.026 0.94
140_V 144_L 1.026 0.94
178_R 207_F 1.025 0.93
361_K 369_R 1.022 0.93
101_G 428_T 1.016 0.93
263_A 310_Q 1.009 0.93
155_Q 265_L 1.007 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3i7fA20.91851000.169Contact Map0.685
4j15A20.92061000.17Contact Map0.706
2xgtA20.9121000.177Contact Map0.748
1eovA10.92921000.177Contact Map0.66
3m4pA40.91851000.186Contact Map0.783
1x54A10.91631000.188Contact Map0.717
3nemA20.90991000.196Contact Map0.803
4ex5A20.90561000.198Contact Map0.713
1c0aA10.93781000.205Contact Map0.721
1l0wA20.88841000.206Contact Map0.8
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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