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OPENSEQ.org
TGT - Queuine tRNA-ribosyltransferase
UniProt: P0A847 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10996
Length: 375 (366)
Sequences: 1727
Seq/Len: 4.72
TGT
Paralog alert: 0.04 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
14_R 350_E 3.882 1.00
47_E 83_K 3.514 1.00
72_L 293_S 3.346 1.00
152_W 193_D 3.314 1.00
167_K 171_E 3.308 1.00
8_T 237_A 3.295 1.00
303_D 336_R 3 1.00
277_L 330_N 2.887 1.00
67_Q 133_D 2.848 1.00
156_K 160_E 2.829 1.00
302_C 307_C 2.792 1.00
106_E 164_R 2.634 1.00
16_R 25_E 2.571 1.00
45_E 328_R 2.538 1.00
347_K 351_E 2.509 1.00
296_G 300_P 2.498 1.00
71_K 133_D 2.498 1.00
345_L 354_L 2.488 1.00
256_V 345_L 2.48 1.00
196_D 200_K 2.379 1.00
4_E 16_R 2.351 1.00
257_R 358_V 2.335 1.00
166_A 207_F 2.3 1.00
303_D 340_R 2.297 1.00
16_R 23_V 2.271 1.00
201_G 204_D 2.22 1.00
123_D 126_K 2.184 1.00
73_H 295_T 2.183 1.00
186_I 235_I 2.133 1.00
192_E 226_R 2.117 1.00
125_E 168_R 2.099 1.00
223_D 226_R 2.082 1.00
281_D 364_R 2.064 1.00
304_C 307_C 2.056 1.00
47_E 84_G 2.054 1.00
70_M 134_L 2.052 1.00
152_W 156_K 2.048 1.00
163_L 198_S 2.019 1.00
18_V 23_V 2.009 1.00
257_R 354_L 1.999 1.00
6_D 16_R 1.983 1.00
160_E 197_I 1.921 1.00
296_G 312_R 1.891 1.00
136_S 139_V 1.89 1.00
229_E 258_R 1.876 1.00
155_A 189_S 1.866 1.00
212_V 260_I 1.851 1.00
62_W 120_I 1.843 1.00
163_L 201_G 1.839 1.00
9_D 352_G 1.827 1.00
199_V 231_V 1.825 1.00
341_L 357_F 1.816 1.00
25_E 52_Q 1.814 1.00
146_T 155_A 1.809 1.00
40_G 324_I 1.8 1.00
197_I 201_G 1.793 1.00
343_A 347_K 1.777 1.00
291_Y 294_D 1.759 1.00
156_K 197_I 1.756 1.00
129_E 172_R 1.736 1.00
36_G 80_M 1.733 1.00
44_E 310_Y 1.715 0.99
289_A 292_K 1.704 0.99
256_V 342_M 1.704 0.99
306_T 332_I 1.673 0.99
166_A 202_L 1.669 0.99
336_R 340_R 1.658 0.99
22_G 85_P 1.637 0.99
4_E 18_V 1.633 0.99
360_D 364_R 1.631 0.99
301_E 364_R 1.62 0.99
151_D 154_Y 1.616 0.99
126_K 129_E 1.59 0.99
76_L 86_I 1.579 0.99
344_G 357_F 1.575 0.99
112_R 119_P 1.542 0.99
220_P 223_D 1.534 0.99
196_D 234_Q 1.512 0.98
340_R 344_G 1.508 0.98
113_N 116_N 1.504 0.98
49_T 335_L 1.498 0.98
167_K 205_I 1.496 0.98
357_F 361_F 1.484 0.98
340_R 343_A 1.483 0.98
144_E 154_Y 1.438 0.98
305_Y 309_N 1.432 0.98
196_D 230_H 1.43 0.97
172_R 175_S 1.43 0.97
141_I 169_S 1.424 0.97
6_D 14_R 1.421 0.97
68_E 133_D 1.404 0.97
254_E 258_R 1.382 0.97
81_Q 310_Y 1.376 0.97
153_D 156_K 1.365 0.96
1_M 181_A 1.364 0.96
200_K 204_D 1.357 0.96
250_E 365_Q 1.343 0.96
192_E 196_D 1.34 0.96
199_V 234_Q 1.323 0.96
14_R 25_E 1.317 0.95
128_M 139_V 1.306 0.95
225_H 254_E 1.299 0.95
253_V 358_V 1.294 0.95
348_A 353_K 1.293 0.95
69_I 293_S 1.29 0.95
280_T 344_G 1.289 0.95
44_E 48_A 1.288 0.95
153_D 157_R 1.283 0.94
324_I 327_A 1.276 0.94
150_A 189_S 1.275 0.94
192_E 230_H 1.275 0.94
42_T 45_E 1.271 0.94
296_G 311_S 1.271 0.94
110_H 119_P 1.256 0.94
2_K 18_V 1.252 0.93
253_V 345_L 1.248 0.93
252_L 255_G 1.244 0.93
345_L 348_A 1.243 0.93
28_C 339_Q 1.24 0.93
197_I 200_K 1.24 0.93
77_H 86_I 1.236 0.93
269_T 331_T 1.229 0.93
52_Q 85_P 1.229 0.93
141_I 162_S 1.227 0.93
221_K 251_D 1.22 0.92
184_G 207_F 1.214 0.92
127_S 131_Q 1.213 0.92
353_K 356_S 1.211 0.92
69_I 72_L 1.196 0.91
167_K 204_D 1.195 0.91
319_D 326_G 1.183 0.91
128_M 142_F 1.181 0.91
359_T 363_Q 1.177 0.90
43_P 310_Y 1.174 0.90
277_L 286_I 1.161 0.90
25_E 350_E 1.16 0.90
280_T 340_R 1.157 0.89
78_D 83_K 1.154 0.89
9_D 349_I 1.149 0.89
227_I 230_H 1.146 0.89
110_H 121_F 1.146 0.89
252_L 345_L 1.142 0.89
73_H 313_A 1.141 0.88
141_I 166_A 1.132 0.88
36_G 56_G 1.128 0.88
353_K 360_D 1.128 0.88
53_I 138_I 1.123 0.87
129_E 133_D 1.114 0.87
81_Q 309_N 1.112 0.87
361_F 365_Q 1.111 0.87
277_L 298_L 1.11 0.87
190_V 216_A 1.108 0.86
17_L 24_V 1.101 0.86
128_M 169_S 1.101 0.86
287_R 319_D 1.1 0.86
243_L 246_V 1.096 0.86
62_W 122_L 1.092 0.85
272_A 332_I 1.084 0.85
276_H 283_V 1.084 0.85
302_C 333_H 1.079 0.84
337_Y 341_L 1.078 0.84
319_D 330_N 1.077 0.84
316_H 320_R 1.072 0.84
144_E 158_S 1.068 0.84
46_V 49_T 1.067 0.83
155_A 191_Y 1.066 0.83
286_I 319_D 1.061 0.83
280_T 364_R 1.058 0.83
307_C 333_H 1.054 0.82
284_V 291_Y 1.053 0.82
347_K 350_E 1.053 0.82
257_R 355_E 1.051 0.82
105_T 121_F 1.051 0.82
42_T 328_R 1.036 0.81
243_L 260_I 1.033 0.81
183_F 262_M 1.033 0.81
159_M 163_L 1.033 0.81
280_T 299_D 1.027 0.80
305_Y 332_I 1.02 0.80
170_R 205_I 1.018 0.79
148_Y 190_V 1.016 0.79
243_L 255_G 1.014 0.79
271_N 274_N 1.012 0.79
41_M 332_I 1.011 0.79
50_G 84_G 1.005 0.78
130_I 134_L 1.004 0.78
280_T 301_E 1.004 0.78
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4e2vA10.98671000.025Contact Map0.712
2ashA40.9841000.037Contact Map0.781
1iq8A20.9041000.162Contact Map0.719
1vcfA20.7284.60.962Contact Map0.385
3ewbX10.6875.30.965Contact Map0.55
1nvmA40.725363.40.968Contact Map0.559
4jn6A20.730754.80.969Contact Map0.418
2nzlA10.7387530.97Contact Map0.448
2nuwA20.50452.20.97Contact Map0.474
2nx9A20.738750.50.97Contact Map0.522
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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