OPENSEQ.org - RL36 - ECOLI GREMLIN

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RL36 - 50S ribosomal protein L36

UniProt:	P0A7Q6	Sequence MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG11232
Length:	38 (38)
Sequences:	509
Seq/Len:	13.39

RL36
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
Cluster includes: RL362 RL36

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
13_N	28_S	3.869	1.00
12_R	30_E	2.651	1.00
11_C	33_H	2.336	1.00
11_C	27_C	2.053	1.00
27_C	33_H	1.821	1.00
1_M	26_I	1.46	1.00
4_R	10_L	1.273	0.99
17_V	24_R	1.252	0.99
5_A	15_K	1.205	0.98
3_V	16_I	1.199	0.98
21_G	36_R	1.137	0.97
16_I	25_V	1.107	0.96
13_N	29_A	1.074	0.95
3_V	7_V	1.065	0.95
14_C	27_C	1.045	0.94
21_G	28_S	1.012	0.93

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
3bbo6	1	1	99.7	-0.008	Contact Map	0.392
4kix4	1	1	99.7	0.039	Contact Map	0.612
2zjr4	1	0.9737	99.6	0.061	Contact Map	0.599
2napA	1	0.8684	2.7	0.889	Contact Map	0.038
3py7A	1	0.6316	2.3	0.893	Contact Map	0
3w1yA	2	1	2.3	0.893	Contact Map	0.085
1jc9A	1	0.3421	2.2	0.894	Contact Map	0
3ml1A	1	0.8947	2.1	0.896	Contact Map	0.056
2vpzA	2	0.8947	1.9	0.898	Contact Map	0
3h47A	1	0.7105	1.8	0.9	Contact Map	0

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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