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OPENSEQ.org
PROB - Glutamate 5-kinase
UniProt: P0A7B5 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10768
Length: 367 (365)
Sequences: 1217
Seq/Len: 3.33
PROB
Paralog alert: 0.03 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
6_T 44_R 3.894 1.00
280_V 315_I 3.82 1.00
315_I 359_V 3.799 1.00
53_I 144_I 3.62 1.00
282_E 318_L 3.514 1.00
9_V 35_C 3.063 1.00
341_H 349_I 2.992 1.00
77_A 99_G 2.77 1.00
300_I 358_A 2.738 1.00
278_I 313_I 2.713 1.00
13_T 54_A 2.713 1.00
177_A 182_N 2.703 1.00
199_R 222_D 2.691 1.00
176_T 182_N 2.683 1.00
335_L 358_A 2.671 1.00
322_D 325_H 2.611 1.00
39_H 128_N 2.482 1.00
314_R 322_D 2.456 1.00
46_V 160_A 2.418 1.00
280_V 285_T 2.369 1.00
173_G 187_L 2.341 1.00
121_L 133_I 2.337 1.00
336_R 339_A 2.319 1.00
190_D 256_H 2.296 1.00
285_T 317_N 2.232 1.00
279_T 302_S 2.222 1.00
341_H 345_E 2.22 1.00
244_D 249_I 2.211 1.00
15_V 236_G 2.202 1.00
238_K 244_D 2.191 1.00
277_E 316_C 2.189 1.00
166_L 242_I 2.179 1.00
36_A 96_I 2.131 1.00
174_L 191_V 2.115 1.00
178_D 181_S 2.108 1.00
191_V 255_F 2.081 1.00
230_D 257_A 2.053 1.00
317_N 321_R 1.993 1.00
117_A 159_L 1.991 1.00
277_E 304_T 1.991 1.00
285_T 323_I 1.967 1.00
32_V 90_L 1.956 1.00
313_I 328_S 1.949 1.00
334_A 352_Y 1.838 0.99
107_D 113_R 1.831 0.99
329_R 363_D 1.83 0.99
176_T 186_E 1.797 0.99
191_V 253_T 1.784 0.99
279_T 304_T 1.766 0.99
35_C 47_I 1.741 0.99
286_A 290_E 1.617 0.98
283_G 299_G 1.613 0.98
68_T 71_S 1.608 0.98
13_T 209_L 1.569 0.97
101_M 138_A 1.542 0.97
301_K 318_L 1.541 0.97
47_I 87_W 1.54 0.97
11_L 47_I 1.54 0.97
32_V 91_F 1.504 0.97
283_G 287_A 1.5 0.96
312_V 327_V 1.494 0.96
111_R 115_L 1.489 0.96
91_F 98_V 1.477 0.96
39_H 96_I 1.476 0.96
306_N 336_R 1.469 0.96
280_V 288_I 1.444 0.95
13_T 153_S 1.438 0.95
283_G 296_L 1.41 0.94
33_R 93_I 1.398 0.94
13_T 22_R 1.393 0.94
179_P 185_A 1.38 0.94
60_L 78_V 1.377 0.94
172_K 235_A 1.371 0.93
230_D 254_L 1.371 0.93
277_E 314_R 1.368 0.93
286_A 291_R 1.341 0.92
28_I 86_L 1.338 0.92
96_I 130_V 1.337 0.92
85_Q 89_Q 1.334 0.92
275_A 311_E 1.333 0.92
41_A 247_E 1.326 0.92
177_A 184_Q 1.314 0.91
225_C 231_T 1.301 0.91
77_A 138_A 1.3 0.91
37_Q 40_A 1.288 0.90
284_A 295_L 1.27 0.89
279_T 318_L 1.267 0.89
29_V 90_L 1.266 0.89
326_G 359_V 1.265 0.89
338_I 346_I 1.264 0.89
266_K 292_G 1.256 0.89
192_Y 256_H 1.25 0.88
166_L 245_V 1.246 0.88
37_Q 243_G 1.229 0.87
31_L 87_W 1.224 0.87
120_T 138_A 1.212 0.86
71_S 123_A 1.21 0.86
133_I 138_A 1.198 0.85
281_D 299_G 1.193 0.85
297_P 346_I 1.192 0.85
296_L 299_G 1.191 0.85
65_L 72_K 1.186 0.85
196_D 203_G 1.182 0.84
151_N 219_Q 1.18 0.84
23_L 55_A 1.173 0.84
29_V 33_R 1.169 0.83
204_D 211_T 1.167 0.83
7_L 45_I 1.166 0.83
360_H 364_M 1.162 0.83
29_V 93_I 1.157 0.82
353_E 356_P 1.155 0.82
280_V 323_I 1.148 0.82
35_C 45_I 1.144 0.81
234_A 241_V 1.143 0.81
284_A 299_G 1.137 0.81
153_S 165_L 1.136 0.81
17_T 24_N 1.133 0.81
341_H 344_Q 1.13 0.80
14_S 210_G 1.126 0.80
106_A 110_D 1.124 0.80
94_Y 98_V 1.123 0.80
175_Y 179_P 1.123 0.80
101_M 121_L 1.113 0.79
7_L 38_L 1.109 0.79
168_L 234_A 1.105 0.78
188_I 201_I 1.094 0.77
120_T 124_L 1.093 0.77
170_D 185_A 1.092 0.77
340_G 343_S 1.086 0.77
294_S 360_H 1.082 0.76
46_V 133_I 1.077 0.76
151_N 223_V 1.068 0.75
243_G 247_E 1.061 0.74
57_R 66_P 1.059 0.74
72_K 211_T 1.047 0.73
303_V 335_L 1.046 0.73
294_S 299_G 1.046 0.73
266_K 362_D 1.042 0.72
57_R 65_L 1.04 0.72
91_F 130_V 1.039 0.72
177_A 183_P 1.038 0.72
359_V 364_M 1.037 0.72
8_V 162_A 1.036 0.72
339_A 366_T 1.035 0.72
242_I 245_V 1.035 0.72
334_A 337_R 1.034 0.71
235_A 238_K 1.033 0.71
81_S 84_I 1.032 0.71
34_Q 241_V 1.027 0.71
281_D 296_L 1.023 0.70
279_T 316_C 1.02 0.70
73_Q 116_N 1.016 0.70
33_R 94_Y 1.015 0.69
141_T 145_K 1.008 0.69
165_L 231_T 1.006 0.68
11_L 31_L 1.006 0.68
329_R 355_G 1.005 0.68
292_G 361_R 1.001 0.68
278_I 315_I 1.001 0.68
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2j5vA211000.253Contact Map0.792
2akoA40.68121000.608Contact Map0.692
3d40A10.70571000.661Contact Map0.53
3ll9A20.68121000.662Contact Map0.6
2e9yA20.6731000.673Contact Map0.426
1z9dA30.66211000.674Contact Map0.652
1e19A20.66491000.677Contact Map0.553
3ll5A40.64581000.681Contact Map0.625
3k4oA20.6731000.684Contact Map0.545
3nwyA60.62941000.687Contact Map0.682
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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